Genomic DNA (gDNA) was extracted using a Qiagen Genomic-tip 100 G−1 kit (cat. no. 10243; Qiagen) according to the manufacturer’s instructions for yeast, with minor modifications. Zymolyase 20T (MP Biomedicals; 1000 U ml−1 in distilled water) was increased to 500 µL and added to Kazachstania cells (~ 2×109). The yield and concentration of the eluted DNA was assessed via a NanoDrop One spectrophotometer (Thermo Fisher Scientific). DNA purity and integrity were based on calculation of the A260/A280 and A260/A230 ratios, as well as visually after electrophoresis (0.75 % agarose in tris acetate EDTA (TAE) buffer). gDNA samples with an A260/A280 ratio of 1.8–2.0 [7.5 μg (413.9 ng µl−1; PF_8_W29 and 56.2 ng µl−1; PF_9_W20) for each sample] were submitted to the South Australian Genomics Centre (SAGC) (Adelaide, Australia) for PacBio sequencing.
Genomic tip 100 g 1 kit
Manufactured by Qiagen
The Genomic-tip 100 G−1 kit is a laboratory equipment product designed for the purification of high-molecular-weight genomic DNA from various sample sources. The kit utilizes anion-exchange chromatography to efficiently capture and purify genomic DNA.
Automatically generated - may contain errors
Lab products found in correlation
Qubit fluorometer, by Thermo Fisher Scientific (2 mentions)
Nanodrop one spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Zymolyase 20t, by MP Biomedicals (1 mentions)
Promethion flow cell, by Oxford Nanopore (1 mentions)
Agilent genomic dna screentape assay, by Agilent Technologies (1 mentions)
Qubit dsdna br assay kit, by Thermo Fisher Scientific (1 mentions)
Tapestation 4200, by Agilent Technologies (1 mentions)
2 protocols using genomic tip 100 g 1 kit
1
Genomic DNA Extraction from Yeast
2
Nanopore Sequencing of Genomic DNA
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DNA extraction for nanopore sequencing
Genomic DNA was extracted from frozen liver/spleen tissue that had been ground into a fine powder under liquid nitrogen. The Qiagen Genomic Tip (100 G -1 ) kit (Qiagen; 10243) was used to extract DNA from 100 mg of tissue powder. DNA was quantified using the Qubit Fluorometer (Thermo Fisher Scientific) and the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific; Q32853) and the molecular weight was assessed using the Agilent TapeStation 4200 and the Agilent Genomic DNA ScreenTape Assay (Agilent; 5067-5365 and 5067-5366). A sequencing library was prepared using the Genomic DNA by Ligation Kit (Oxford Nanopore Technologies; SQK-LSK109) and run on a PromethION flow cell (Oxford Nanopore Technologies; FLO-PRO002) for 72 h on a PromethION beta sequencer (Oxford Nanopore Technologies).
Genomic DNA was extracted from frozen liver/spleen tissue that had been ground into a fine powder under liquid nitrogen. The Qiagen Genomic Tip (100 G -1 ) kit (Qiagen; 10243) was used to extract DNA from 100 mg of tissue powder. DNA was quantified using the Qubit Fluorometer (Thermo Fisher Scientific) and the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific; Q32853) and the molecular weight was assessed using the Agilent TapeStation 4200 and the Agilent Genomic DNA ScreenTape Assay (Agilent; 5067-5365 and 5067-5366). A sequencing library was prepared using the Genomic DNA by Ligation Kit (Oxford Nanopore Technologies; SQK-LSK109) and run on a PromethION flow cell (Oxford Nanopore Technologies; FLO-PRO002) for 72 h on a PromethION beta sequencer (Oxford Nanopore Technologies).
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