96-well plates (Corning Clear Polystyrene 96-Well Microplates) were coated overnight with HDM extracts at 200 μg/mL in 0.05 M Na2CO3 pH 9.6. Coated plates were then blocked for 1 h with 1% BSA in PBS. Serum from mice was collected and serially diluted (threefold) in PBS with 10 mg/mL BSA and 0.1% Tween 20 before incubation on coated plates. After washing, bound antibody was detected with HRP-conjugated goat Anti-Mouse IgE (1:2,000, 1110-05; Southern Biotech) and quantified by spectrophotometry at 405 nm (OD).
Hrp conjugated goat anti mouse ige
Manufactured by Southern Biotech
Sourced in United States
HRP-conjugated goat Anti-Mouse IgE is a laboratory reagent used for the detection and quantification of mouse immunoglobulin E (IgE) in various immunoassays. It consists of goat-derived antibodies specific to mouse IgE that are conjugated to horseradish peroxidase (HRP), an enzyme that serves as a reporter molecule.
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Lab products found in correlation
3 protocols using hrp conjugated goat anti mouse ige
1
Mouse IgE Antibody ELISA Protocol
2
Quantifying Allergen-Specific IgE in Mice
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Ninety-six-well plates (Corning Clear Polystyrene 96-Well Microplates) were coated overnight with HDM extracts at 200 μg/ml in 0.05 M Na2CO3 pH 9.6. The coated plates were then blocked for 1 h with 1% BSA in PBS. Serum from mice was collected and serially diluted (threefold) in PBS with 10 mg/ml BSA and 0.1% Tween 20 before incubation in the coated plates. After washing, bound antibody was detected with HRP-conjugated goat anti-mouse IgE (1:2,000, 1110-05; Southern Biotech, Birmingham, AL, U.S.) and quantified by spectrophotometry at 405 nm (OD).
3
Mouse IgE Antibody ELISA Protocol
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