Human mesenchymal stem cells (MSC) and osteoblasts were obtained from Lonza Group Ltd (hereafter referred to as “Lonza”). Mesenchyme cells were grown and maintained in Mesenchymal Cell Growth Supplement (MCGS, PT-3001) with L-glutamine and containing 30 mg/ml gentamicin, 15 ug/ml amphotericin and 10% fetal bovine serum (FBS). Cells were cultured at 37 °C in the presence of 5% CO2 until ∼75% confluent. Mesenchyme cells were induced to differentiate to osteoblasts by culturing the cells in the presence of osteogenic medium from Lonza according to the manufacturer's protocol. Human primary osteoblasts were also obtained from Lonza. The primary osteoblasts were cultured in Clonetics Osteoblast Growth Medium especially formulated for the growth and expansion of human osteoblasts, and contained 10% FBS, ascorbic acid and gentamicin-amphotericin.
Osteoblast growth medium
Manufactured by Cambrex
Osteoblast Growth Medium is a cell culture medium specifically formulated to support the growth and proliferation of osteoblast cells. It provides the necessary nutrients and growth factors required for the maintenance and expansion of these cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using osteoblast growth medium
1
Differentiation of Mesenchymal Stem Cells to Osteoblasts
2
Differentiation of Mesenchymal Stem Cells to Osteoblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human mesenchymal stem cells (MSC) and osteoblasts were obtained from Lonza Group Ltd (hereafter referred to as “Lonza”). Mesenchyme cells were grown and maintained in Mesenchymal Cell Growth Supplement (MCGS, PT-3001) with L-glutamine and containing 30 mg/ml gentamicin, 15 ug/ml amphotericin and 10% fetal bovine serum (FBS). Cells were cultured at 37 °C in the presence of 5% CO2 until ∼75% confluent. Mesenchyme cells were induced to differentiate to osteoblasts by culturing the cells in the presence of osteogenic medium from Lonza according to the manufacturer's protocol. Human primary osteoblasts were also obtained from Lonza. The primary osteoblasts were cultured in Clonetics Osteoblast Growth Medium especially formulated for the growth and expansion of human osteoblasts, and contained 10% FBS, ascorbic acid and gentamicin-amphotericin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!