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The SupT1 is a cell line product from Thermo Fisher Scientific. It is a human T cell line derived from a patient with acute lymphoblastic leukemia. The SupT1 cell line can be used for various cell biology and immunology research applications.

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Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Sup t1, by American Type Culture Collection (2 mentions) 293t cell, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Imr 90, by American Type Culture Collection (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hek293a, by Thermo Fisher Scientific (1 mentions) Human aortic endothelial cells, by Lonza (1 mentions) Rpmi 1640 glutamax culture medium, by Thermo Fisher Scientific (1 mentions) Z 138, by American Type Culture Collection (1 mentions) Jvm 2, by American Type Culture Collection (1 mentions) Tib 152, by American Type Culture Collection (1 mentions) Su dhl2, by Thermo Fisher Scientific (1 mentions) Su dhl 2, by American Type Culture Collection (1 mentions) Su dhl 6, by American Type Culture Collection (1 mentions) Jeko 1, by American Type Culture Collection (1 mentions) Ccl 119, by American Type Culture Collection (1 mentions) Rec 1, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

4 protocols using supt1

1

Cell Culture Protocols for Diverse Immune Cell Lines

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293T, IMR90, and SupT1 cells were purchased from the American Type Culture Collection. K562-CD19 and K562-mesothelin cells were provided by J. Fraietta. 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), supplemented with 10% fetal bovine serum (FBS; Gibco) and 5% penicillin-streptomycin (Gibco) in a humidified incubator at 37°C. SupT1 and K562 cells were cultured in RPMI 1640 (Gibco) supplemented with 10% FBS and 5% penicillin-streptomycin, incubated as above. IMR90 cells were cultured in DMEM supplemented with 10% FBS and 5% penicillin-streptomycin, cultured under physiological oxygen (3%).
Collins S.M., Alexander K.A., Lundh S., Dimitri A.J., Zhang Z., Good C.R., Fraietta J.A, & Berger S.L. (2023). TOX2 coordinates with TET2 to positively regulate central memory differentiation in human CAR T cells. Science Advances, 9(29), eadh2605.
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2

Cell Culture Protocols for Cancer Research

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Human astrocytoma cell line U251 and T cell CD4+ lymphoblastic lymphoma cell line SUPT1 were obtained from Sigma-Aldrich (Schnelldorf, Germany). Human Embryonic Kidney 293A (HEK293A) and adenocarcinoma cervix epithelial HeLa were obtained from ATCC (ATCC-LGC standards GmbH, Wesel, Germany). Human Aortic Endothelial Cells (HAEC) were obtained from Lonza and grew in EGM medium (Lonza, Basel Switzerland). HeLa and HEK293A were cultivated in DMEM medium supplemented with Streptomycin/penicillin antibiotic solution (Gibco BRL-Life Technologies, Grand Island, NY) and with 10% Foetal Bovine serum (FBS) (Gibco BRL). U251 was cultivated in RPMI-1640-GlutaMax culture medium with 1% FBS (Gibco BRL) and SUPT1 in RPMI-1640-GlutaMax culture medium with 10% FBS.
Satta N., Weppe R., Pagano S., Frias M., Juillard C, & Vuilleumier N. (2020). Auto-antibodies against apolipoprotein A-1 block cancer cells proliferation and induce apoptosis. Oncotarget, 11(46), 4266-4280.
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3

Culturing SupT1 T Lymphoblasts

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The human T lymphoblastic cell line SupT1 (ATCC CRL-1942) was grown at 37 °C in 5% CO2 incubator in RPMI medium (RPMI 1640, Life Technologies), completed with 10% heat-inactivated fetal bovine serum (FBS), 1% l-Glutamine (200 nm final concentration) and 1% penicillin–streptomycin (100 UmL). Cells were seeded at a density of 0.5 × 106 cells/mL and sub-cultured when density reached ~ 2–3 × 106 cells/mL.
Zhang Y., Liu W., Li Z., Kumar V., Alvarez-Cabrera A.L., Leibovitch E.C., Cui Y., Mei Y., Bi G.Q., Jacobson S, & Zhou Z.H. (2019). Atomic structure of the human herpesvirus 6B capsid and capsid-associated tegument complexes. Nature Communications, 10, 5346.
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4

TCL and BCL Cell Line Characterization

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H9, HH, C5MJ, J. Cam 1.6, SUP-T1, Tib152, and CCL119 are TCL cell lines purchased from the ATCC. SU-DHL6, SU-DHL2, Jeko-1, JVM-2, Z-138, Rec-1 are characterized BCL lines purchased from the ATCC. DND41 is a T-cell line purchased from Deutsche Sammlung von Mikroorganism und Zellkulturen GmbH (DSMZ; Braunschweig, Germany). OCI-LY10 and OCI-LY7 are BCL cell lines from DSMZ. All cell lines were grown in RPMI-1640 (H9, HH, J. Cam 1.6, SUP-T1, Tib-152, CCL119, DND41, Jeko-1, JVM-2, Z-138, Rec-1, SU-DHL6, and SU-DHL2) or IMDM media (C5MJ, OCI-LY10, and OCI-LY7) with 10% FBS (Life Technology) and maintained at a concentration of 0.3 × 106 cells/mL. All cell lines were authenticated from a hematopathologist, including verification of morphology and immunophenotype (11 (link)–13 (link)).
Zullo K.M., Guo Y., Cooke L., Jirau-Serrano X., Mangone M., Scotto L., Amengual J.E., Mao Y., Nandakumar R., Cremers S., Duong J., Mahadevan D, & O’Connor O.A. (2015). Aurora A Kinase Inhibition Selectively Synergizes with Histone Deacetylase Inhibitor through Cytokinesis Failure in T-cell Lymphoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(18), 4097-4109.
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