Protein was isolated by SDS-PAGE gel and transferred to PVDF membrane. After 1 h of blocking in 5% skim milk, the membrane was incubated with various primary antibodies and incubated overnight at 4 °C with Anti-Alix (sc-53540, Santa Cruz), Anti-α-SMA (ab7817, Abcam), Anti-CD63 (ab134045, Abcam), Anti-FAP (ab207178, Abcam), Anti-ICAM1 (ab282575, Abcam), Anti-MMP-9 (ab76003, Abcam), Anti-MMP-3 (ab52915, Abcam), Anti-β-catenin (ab32572, Abcam), Anti-Ki67 (ab15580, Abcam), Anti-PCNA (ab92552, Abcam), Anti-MMP-2 (ab92536, Abcam), Anti-PDGFR beta (ab69506, Abcam), Anti‐β‐actin (A5441, Sigma), Anti-E-cadherin (SAB4503751, Sigma), Anti-N-cadherin (C3865, Sigma), Anti-Vimentin (V6389, Sigma), Anti-snail (SAB1306281, Sigma), Anti-Zeb1 (SAB2500097, Sigma), Anti-Zeb2 (SAB3500515, Sigma), Anti-Slug (PRS3959, Sigma), Anti‐HSP70 (4873, Cell Signaling Technology), Anti‐GM130 (G7295, Sigma), Anti-FUS (DF8391, Affinity), Anti-ACO1 (AF4711, Affinity), Anti-YTHDC1 (77422, Cell Signaling Technology), Anti-TCEAL7 (DF9969, Affinity). And after three times of elution with TBST, the secondary antibody linked with horseradish peroxidase was incubated for 1 h and the electrochemical luminescence was observed by chemiluminescence apparatus. Results quantization was performed by ImageJ software.
Anti slug
Manufactured by Merck Group
Anti-Slug is a laboratory equipment product developed by Merck Group. It is designed to remove slugs and other small pests from laboratory environments. The core function of Anti-Slug is to effectively capture and contain these unwanted organisms, maintaining a clean and organized work space.
Automatically generated - may contain errors
Lab products found in correlation
Anti e cadherin, by Merck Group (2 mentions)
Anti hsp70, by Cell Signaling Technology (2 mentions)
Anti gm130, by Merck Group (2 mentions)
Anti n cadherin, by Merck Group (2 mentions)
Anti alix, by Santa Cruz Biotechnology (2 mentions)
Anti snail, by Merck Group (2 mentions)
Anti zeb2, by Merck Group (2 mentions)
Anti vimentin, by Merck Group (2 mentions)
Anti β actin, by Merck Group (2 mentions)
Ab76003, by Abcam (1 mentions)
Ab52915, by Abcam (1 mentions)
Ab282575, by Abcam (1 mentions)
Anti ythdc1, by Cell Signaling Technology (1 mentions)
Anti icam 1, by Abcam (1 mentions)
Anti fap, by Abcam (1 mentions)
Anti zeb1, by Merck Group (1 mentions)
Anti α sma, by Abcam (1 mentions)
Anti cd63, by Abcam (1 mentions)
Anti mmp9, by Abcam (1 mentions)
2 protocols using anti slug
1
Comprehensive Protein Expression Analysis
2
Comprehensive Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein was isolated by SDS-PAGE gel and transferred to PVDF membrane. After 1 h of blocking in 5% skim milk, the membrane was incubated with various primary antibodies and incubated overnight at 4℃ with Anti-Alix (1: 1000, Santa Cruz), Anti-α-SMA (1: 1000, Abcam), Anti-CD63 (1:2000, Abcam), Anti-FAP (1: 1000, Abcam), Anti-ICAM1 (1: 1000, Abcam), Anti-MMP9 (1: 1000, Abcam), Anti-PDGFR beta(1: 1000, Abcam), Anti-β-actin(1: 1000, Sigma), Anti-E-cadherin (1: 1000, Sigma), Anti-N-cadherin(1: 1000, Sigma), Anti-Vimentin(1: 1000, Sigma), Anti-snail (1: 1000, Sigma), Anti-Zeb1(1: 1000, Sigma), Anti-Zeb2(1: 1000, Sigma), Anti-Slug(1: 1000, Sigma), Anti-HSP70 (1: 1000,Cell Signaling Technology), Anti-GM130(1: 1000, Sigma), Anti-ZRANB2(1: 1000, A nity), Anti-ACO1(1: 1000, A nity), Anti-MBNL1(1: 1000, A nity), Anti-TCEAL7(1: 1000, A nity). And after three times of elution with TBST the secondary antibody linked with horseradish peroxidase was incubated for 1 hour and the electrochemical luminescence was observed by chemiluminescence apparatus. Results WB quantization was performed by ImageJ software.
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