Thai-53 strain of M. leprae [20] (link), maintained at the Leprosy Research Center, National Institute of Infectious Diseases (Tokyo, Japan), was used for the preparation of M. leprae DNA. Escherichia coli strain TOP-10 (Thermos Fisher Scientific, Waltham, MA) was used for cloning. While E. coli strains Rosetta-gami TM 2(DE3)pLysS and BL21(DE3)pLysS (Merck KGaA, Darmstadt, Germany) were used for protein expression. Further, plasmid vector pET20b(+) (Merck KGaA) was used to construct expression plasmids, and relaxed pBR322 plasmid DNA (Inspiralis Ltd., Norwich, UK) was used for the assessment of supercoiling activity.
Rosetta gami tm 2 de3 plyss
Manufactured by Merck Group
Sourced in Germany
Rosetta-gami TM 2(DE3)pLysS is a bacterial expression strain developed for the production of recombinant proteins. It is derived from the BL21(DE3)pLysS strain and is designed to enhance the expression of proteins that contain rare codons found in E. coli.
Automatically generated - may contain errors
2 protocols using rosetta gami tm 2 de3 plyss
1
Preparation and Expression of M. leprae DNA
2
M. leprae DNA Preparation and Expression
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The Thai-53 strain of M. leprae [20] (link), maintained at the Leprosy Research Center, National Institute of Infectious Diseases (Tokyo, Japan), was used to prepare M. leprae DNA. Escherichia coli strain TOP-10 (Thermos Fisher Scientific Inc.; Waltham, MA) was used for cloning. E. coli strains Rosetta-gami TM 2(DE3)pLysS and BL21(DE3)pLysS (Merck KGaA, Darmstadt, Germany) were used for protein expression. The plasmid vector pET20b(+) (Merck KGaA) was used to construct expression plasmids. Relaxed pBR322 DNA (Inspiralis Ltd.; Norwich, UK) was used for the DNA supercoiling assay.
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