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Anti runx2

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Anti-RUNX2 is a laboratory research antibody that binds to the RUNX2 protein. RUNX2 is a transcription factor that plays a key role in osteoblast differentiation and bone formation. The Anti-RUNX2 antibody can be used to detect and quantify RUNX2 expression in various biological samples.

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Lab products found in correlation

Anti abl, by BD (3 mentions) Anti actin, by Santa Cruz Biotechnology (3 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Anti taz, by BD (2 mentions) Cetylpyridium chloride, by Merck Group (1 mentions) Alkaline phosphatase alp assay kit, by Takara Bio (1 mentions) Phospho specific antibodies against, by Cell Signaling Technology (1 mentions) Recombinant mouse s100a4, by ProSpec (1 mentions) Anti β actin ac 74, by Merck Group (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Ascorbic acid, by Merck Group (1 mentions) β glycerophosphate, by Merck Group (1 mentions) Anti p tyr 4g10, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions)

4 protocols using anti runx2

1

Regulation of Osteoblast Differentiation

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Recombinant mouse S100A4 was purchased from Prospec (East Brunswick, NJ, USA). Phosphospecific antibodies against IκB kinase (IKK)α/β (Ser176/180), IκB (Ser32), p65 (Ser536), IKKα/β, IκB, and p65 were obtained from Cell Signaling Technology (Beverly, MA, USA). Antibodies against osterix, laminB, and p65 were acquired from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-β-actin (AC-74) was purchased from Sigma-Aldrich (St. Louis, MO, USA), anti-Runx2 from MBL International (Woburn, MA, USA), the alkaline phosphatase (ALP) assay kit from Takara Bio Inc. (Fukui, Japan). β-Glycerophosphate, cetylpyridium chloride, and ascorbic acid from Sigma-Aldrich, Bay11-7082 from Alexis Biochemicals (Grunberg, Germany), and the Cell Counting Kit-8 (CCK-8) from Dojindo (Kumamoto, Japan).
Kim H., Lee Y.D., Kim M.K., Kwon J.O., Song M.K., Lee Z.H, & Kim H.H. (2017). Extracellular S100A4 negatively regulates osteoblast function by activating the NF-κB pathway. BMB Reports, 50(2), 97-102.
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2

Antibody-based Protein Signaling Analysis

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Unless stated otherwise, all chemicals were purchased from Sigma. Antibodies were obtained from the following sources: anti-pABL (Y245) (Cell Signaling Technology), anti-ABL (BD Pharmingen), anti-Flag M2 (Sigma), anti-Actin (Santa Cruz Biotechnologies), anti-RUNX2 (MBL International) and anti-pTyr (4G10) (EMD Millipore) antibodies. Halt™ Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scientific.
He F., Matsumoto Y., Asano Y., Yamamura Y., Katsuyama T., La Rose J., Tomonobu N., Komalasari N.L., Sakaguchi M., Rottapel R, & Wada J. (2021). RUNX2 Phosphorylation by Tyrosine Kinase ABL Promotes Breast Cancer Invasion. Frontiers in Oncology, 11, 665273.
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3

Antibody Reagents for Cell Analysis

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Unless stated otherwise, all chemicals were purchased from Sigma. Antibodies were obtained from the following sources: anti-ABL, anti-TAZ (BD Pharmingen), anti-RUNX2 (MBL International) and anti-Actin (Santa Cruz Biotechnologies). Halt™ Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scientific.
Asano Y., Matsumoto Y., La Rose J., He F., Katsuyama T., Ziyi W., Tsuji S., Kamioka H., Rottapel R, & Wada J. (2021). Endonuclease increases efficiency of osteoblast isolation from murine calvariae. Scientific Reports, 11, 8502.
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4

Investigating Cellular Signaling Pathways

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Unless stated otherwise, all chemicals were purchased from Sigma. Antibodies were obtained from the following sources: anti-ABL, anti-TAZ (BD Pharmingen), anti-RUNX2 (MBL International) and anti-Actin (Santa Cruz Biotechnologies). Halt™ Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scienti c.
Asano Y., Matsumoto Y., Rose J.L., He F., Katsuyama T., Wang Z., Tsuji S., Kamioka H., Rottapel R., & Wada J. (2020). Endonuclease Increases Efficiency of Osteoblast Isolation from Murine Calvariae.
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