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2 protocols using ab15858

1

Antibody Labeling for Organelle Visualization

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Affinity-purified rabbit anti-AQP5 IgG antibody targeted toward amino acid residues 249 to 265 of rat AQP5 (AB15858) and normal goat IgG antibody (NI02-100UG) were obtained from MilliporeSigma. Normal rabbit IgG antibodies (2729) were obtained from Cell Signaling Technology (Danvers, MA, USA). Goat anti-calnexin IgG (LS-B4403) was obtained from LifeSpan BioSciences (Dallas, TX, USA). Rabbit anti-LC3B IgG (MBS9435173) was obtained from MyBioSource (San Diego, CA, USA). Rabbit anti-LIMP-2 IgG (NB400-129) was obtained from Novus Biologicals (Centennial, CO, USA). Mouse anti-TOMM20 IgG (ab56783) was obtained from Abcam (Waltham, MA, USA). Goat anti-connexin 50 IgG (sc-20746) and normal mouse IgG (sc-2025) antibody were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-rabbit Alexa Fluor 647, donkey anti-rabbit Alexa Fluor 488, and donkey anti-goat Alexa Fluor 647) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Secondary antibody used for western blotting (goat anti-rabbit DyLight 680, goat anti-mouse DyLight 800, and donkey anti-goat DyLight 800) was obtained from Thermo Fisher Scientific.
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2

Antibody Reagents for Cell Biology

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Affinity-purified rabbit anti-AQP5 IgG antibody targeted towards amino acid residues 249-265 of rat AQP5 (AB15858) and normal goat IgG antibody (NI02-100UG) were obtained from Millipore Sigma. Normal rabbit IgG antibodies were obtained from Cell Signaling Technology (2729; Danvers, MA). Goat anti-calnexin IgG was obtained from LifeSpan Biosciences (LS-B4403; Dallas, TX). Rabbit anti-LC3B IgG was obtained from MyBioSource, Inc. (MBS9435173; San Diego, CA). Rabbit anti-LIMP-2 IgG was obtained from Novus Biologicals, LLC (NB400-129; Centennial, CO). Mouse anti-TOMM20 IgG was obtained from Abcam (ab56783; Waltham, MA). Goat anti-connexin 50 IgG (sc-20746) and normal mouse IgG antibody were obtained from Santa Cruz Biotechnology (sc-2025; Dallas, TX). Secondary antibodies (goat anti-rabbit Alexa Fluor 488, goat anti-rabbit Alexa Fluor 647, donkey anti-rabbit Alexa Fluor 488, and donkey antigoat Alexa Fluor 647) were obtained from Fisher Scientific. Secondary antibody used for Western blotting was obtained from Fisher Scientific (goat anti-rabbit DyLight 680, goat antimouse DyLight 800, and donkey anti-goat DyLight 800).
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