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K 4500 automated hematology analyzer

Manufactured by Sysmex
Sourced in Japan

The Sysmex K-4500 Automated Hematology Analyzer is a laboratory instrument designed to perform complete blood count (CBC) analysis. It automates the process of detecting and enumerating various blood cell types, including red blood cells, white blood cells, and platelets. The K-4500 provides accurate and reliable hematological data to support clinical decision-making.

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2 protocols using k 4500 automated hematology analyzer

1

Comprehensive Hematological Analysis

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Totally 24 hematological parameters were tested using the Sysmex K-4500 Automated Hematology Analyzer (Sysmex Corporation, Kobe, Japan). Specific hematological parameters are as follows: White blood cell (WBC), Red blood cell (RBC), Haemoglobin (HGB), Red blood cell specific volume (HCT), Mean Corpuscular Volume (MCV), Mean corpuscular hemoglobin (MCH), Mean corpuscular hemoglobin concentration (MCHC), Platelet count (PLT), Neutrophils ratio (NETU%), Neutrophil counts (NETU#), Lymphocyte ratio (LYMPH%), Lymphocyte counts (LYMPH#), Monocyte ratio (MONO%), Monocyte counts (MONO#), Eosinophil ratio (EO%), Eosinophil counts (EO#), Basophile ratio (BASO%), Basophile counts (BASO#), Platelet distribution width (PDW), Mean platelet volume (MPV), Red cell distribution width-stand error (RDW-SD), Red cell distribution width-coefficient of variation (RDW-CV), Platelet-large cell ratio (P-LCR), Platelet cubic measure distributing width (PCT).
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2

Comprehensive Blood Analysis Protocol

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The analysis of the level of cadmium in blood was conducted by graphite furnace atomic absorption spectrometry technique using the iCE 3400 AA Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA).
The assessment of blood morphology parameters was conducted using a Sysmex K-4500 Automated Hematology Analyzer (Sysmex Corporation, Kobe, Japan). The following measurements were obtained: white blood cells (WBC) count, red blood cells (RBC) count, hemoglobin (HGB) level, hematocrit (HCT), and platelet (PLT) count.
Serum vitamin D3 concentration was determined using the Euroimmun kit 25-OH Vitamin D, ELISA nr EQ 6411-9601. The 25-OH vitamin D compounds contained in the tested sample are bound by anti-25-OH antibodies on the microplate. Free antibody binding sites are occupied by labelled 25-OH vitamin D molecules. The intensity of the colour formed after addition of the chromogen/substrate solution is measured photometrically, as colour intensity is inversely proportional to the 25-OH vitamin D concentration in the serum or plasma.
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