After 2×105 5637/NC, 5637/TGFBI, T24/NC, and T24/TGFBI cells were seeded into 6-well plates for 12 hrs, cells were cultured in serum-free RPMI 1640 for 36 hrs. Then, 1 mL cultured serum-free medium was precipitated by 0.15% sodium deoxycholate (Sigma-Aldrich, St. Louis, MO, USA) and 7.2% trichloroacetic acid (Guangzhou Chemical Reagent Factory, Guangzhou, China). After washing with 1 mL acetone (Guangzhou Chemical Reagent Factory, Guangzhou, China) for 3 times, the precipitates were dissolved in 40 µL SDS-PAGE buffer and were then detected by Western blotting.
Acetone
Manufactured by Guangzhou Chemical
Sourced in China
Acetone is a colorless, volatile, and flammable liquid commonly used as a solvent in various industrial and laboratory applications. It has a characteristic sweet, pungent odor. Acetone's primary function is to dissolve and disperse a wide range of organic compounds, making it a versatile and essential tool in many chemical and manufacturing processes.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Guangzhou Chemical (27 mentions)
Methanol, by Guangzhou Chemical (19 mentions)
Hydrochloric acid (hcl), by Guangzhou Chemical (19 mentions)
Urea, by Guangzhou Chemical (18 mentions)
Ethanol, by Guangzhou Chemical (10 mentions)
Acetic acid, by Guangzhou Chemical (9 mentions)
Ethyl acetate, by Guangzhou Chemical (9 mentions)
Concentrated sulfuric acid, by Guangzhou Chemical (9 mentions)
Phosphoric acid, by Guangzhou Chemical (9 mentions)
Acetonitrile, by Guangzhou Chemical (9 mentions)
Ammonium acetate, by Guangzhou Chemical (9 mentions)
Ascorbic acid, by Guangzhou Chemical (9 mentions)
Formic acid, by Guangzhou Chemical (9 mentions)
Diethyl ether, by Guangzhou Chemical (9 mentions)
Pyridine, by Guangzhou Chemical (9 mentions)
Toluene, by Guangzhou Chemical (9 mentions)
Anhydrous ethanol, by Guangzhou Chemical (9 mentions)
Sodium deoxycholate, by Merck Group (3 mentions)
Trichloroacetic acid, by Guangzhou Chemical (3 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (3 mentions)
14 protocols using acetone
1
Secretome Profiling of TGFBI-Modulated Cells
2
Phytochemical Profiling and Antioxidant Evaluation
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Acetone, n-hexane, ethyl acetate, concentrated sulfuric acid, sodium hydroxide, phosphoric acid, acetonitrile, ascorbic acid, methanol, formic acid, sodium acetate, acetic acid, hydrochloric acid, iron(III) chloride hexahydrate solution, ammonium acetate, were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). phenolic compound standards [rutin (Rut), quercetin (Que), quercitrin (Quer), isoquercitrin (Iso), chlorogenic acid (ChA), syringic acid (SyA), ferulic acid (FeA), caffeic acid (CaA), resveratrol (Res), epicatechin (Epi), astragalin (Ast), scopoletin (Sco), galangal (Gal), catechuic acid (CatA), vanillic acid (VaA), benzoic acid (BeA), gallic acid (GaA), and protocatechuic acid (PrA)] were purchased from the National Institutes for Food and Drug Control (Beijing, China). 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2ʹ-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Polymer-based Photocrosslinkable Hydrogel Synthesis
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Acrylamide (Am, 99.9%), acryloyl chloride (96%), choline chloride (ChCl, 99%), β-cyclodextrin (β-CD, 98%), dichloromethane (DCM, 99.9%, with molecular sieves, water ≤ 50 ppm), 4-dimethylaminopyridine (DMAP, 99%), N,N-dimethylformamide (DMF, 99.8%, with molecular sieves, water ≤ 50 ppm), 4-(4,6-dimethoxy−1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%), ethylene glycol (EG, 98%), poly(ethylene glycol) (PEG, average Mn = 2000), rhodamine B, and tartrazine (yellow dye) were purchased from Macklin. 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (I2959, ≥98.0%), lithium phenyl(2,4,6-trimethylbenzoyl)phosphinate (LAP, ≥98.0%), lithocholic acid (LCA, >97.0%), N,N’-methylenebisAcrylamide (MBA), sodium bicarbonate (NaHCO3, ≥99.8%), and triethylamine (TEA, ≥99.5%) were purchased from Aladdin. Acetone, diethyl ether and hydrochloric acid (HCl) were purchased from Guangzhou Chemical Reagent Factory (Guangdong, China).
4
Synthesis of Microcapsule-Based Flame Retardant
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Polyamide 6 (PA6, TP4208) was purchased from Zig Sheng Industrial Co., Taiwan, China. Red phosphorus (RP, 70 wt% content of phosphorus) was supplied by Guangzhou YS Flame Retardant Materials Co., Ltd., Guangzhou, China. Tween−80 and β−cyclodextrin (β−CD) were purchased from Beijing HWRK Chem Co., Ltd., Beijing, China. Toluene diisocyanate (TDI) was purchased from Wanhua Chemical Group Co. Ltd., Yantai, China. Pyridine and acetone were purchased from Guangzhou Chemical Reagent Factory, Guangzhou, China. Microcapsules based on melamine borate coated red phosphorus (Mic−MBP) were prepared by our laboratory [23 (link)].
5
HPLC Analysis of TCM Bioactive Compounds
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EMW was purchased from Guangdong Traditional Chinese Medicine Co., Ltd. (Guangzhou, China). Reference standards, including berberine, palmatine, jateorhizine, phellodendrine, magnoflorine, atractylodin, and chlorogenic acid were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Acetonitrile was high-performance liquid chromatography (HPLC) grade, purchased from Merck (Darmstadt, Germany). Other reagents were analytical grade. Acetone was purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). 2,4-Dinitrochlorobenzene (DNCB) was purchased from Shanghai Acmec Biochemical Co., Ltd. (Shanghai, China). Dexamethasone (DEXA) was purchased from Guangzhou Yuanye Biological Co., Ltd. ELISA kit of EGFR, AKT and MAPK were purchased from Jiangsu Meimian industrial Co., Ltd (Yancheng, China).
6
Fabrication of PVDF-PUL Membranes
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PVDF (average Mv~300,000) was imported from Arkema, Paris, France. PUL was bought from Shanghai Aladdin Biochemical Technology, Shanghai, China. N,N-dimethylformamide (DMF, analytical pure) was bought from Shanghai Aladdin Biochemical Technology, Shanghai, China. Glacial acetic acid (ACS, ≥99.7%) was bought from Shanghai Aladdin Biochemical Technology, Shanghai, China. Acetone (analytical pure AR, ≥99.5%) was bought from Guangzhou Chemical Reagent Factory, Guangzhou, China.
7
Cellulose Fabric Modification Protocol
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Sodium hydroxide, urea, ethanol, acetone, and triethylamine (TEA), were acquired from Guangzhou Chemical Reagent Factory. Copper bromide (CB), pentamethyldiethylenetriamine (PMDETA), α-bromoisobutyryl bromide (BiB), dimethylaminopyridine (DMAP), ascorbic acid (Vc), dichloromethane (DCM), and 1-octadecene (1-Oct) were purchased from Aladdin Reagent (Shanghai) Co., Ltd. The activating agent (AA) used to activate the cellulosic fabric was a mixture of sodium hydroxide and urea, combined at a ratio of m(NaOH) : m((NH2)2CO) = 3 : 2. We made this AA ourselves in the laboratory. The cotton fabric, made of unbleached 100% cotton fiber, commonly used as a filter material for solid–liquid separation, was purchased from a commercial supplier.
8
Synthesis of Silane-Functionalized Benzaldehyde
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Benzaldehyde, malononitile, (3-chloropropyl)trimethoxysilane, and ethylenediamine were obtained from the J & K Chemical Company; THF, acetone, MeOH, EtOH, and other agents were all purchased from Guangzhou Chemical Reagent Factory. The purity of these chemicals is more than 98% and used without purification.
SCB was obtained from local Farm (Guigang, Guangxi Province, China). The primary ingredients of these SCB were analyzed according to TAPPI test method and listed inTable S1 . Prior to use, SCB were dried under vacuum at 80 °C for 24 h. After being milled and sieved, the powder with particle sizes between 40–60 mesh was selected.
SCB was obtained from local Farm (Guigang, Guangxi Province, China). The primary ingredients of these SCB were analyzed according to TAPPI test method and listed in
9
Perovskite Solar Cell Fabrication
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Anhydrous ethanol (99%),
acetone (99%), toluene (99%), and isopropyl alcohol (99%) were purchased
from Guangzhou Chemical Reagent Factory. Ethyl acetate (99.5%), N,N-dimethylformamide (99.9%), and dimethyl
sulfoxide (99.9%) were purchased from Aladdin. Tin(II) chloride dihydrate
(98%) was purchased from Macklin. Lead iodide (99.9%) and methylammonium
iodide (99.5%) were purchased from Xi’an Baolite. High-temperature
carbon slurry with a surface resistivity of 20–30 Ω·sq–1 was purchased from Shanghai Maitewen. FTO conductive
glass was purchased from Enbu, with a surface resistivity of 8 Ω·sq–1. All reagents were used as received from Maitewen
without further purification. Ultrapure water was used for all experimental
steps.
acetone (99%), toluene (99%), and isopropyl alcohol (99%) were purchased
from Guangzhou Chemical Reagent Factory. Ethyl acetate (99.5%), N,N-dimethylformamide (99.9%), and dimethyl
sulfoxide (99.9%) were purchased from Aladdin. Tin(II) chloride dihydrate
(98%) was purchased from Macklin. Lead iodide (99.9%) and methylammonium
iodide (99.5%) were purchased from Xi’an Baolite. High-temperature
carbon slurry with a surface resistivity of 20–30 Ω·sq–1 was purchased from Shanghai Maitewen. FTO conductive
glass was purchased from Enbu, with a surface resistivity of 8 Ω·sq–1. All reagents were used as received from Maitewen
without further purification. Ultrapure water was used for all experimental
steps.
10
Synthesis of Cellulose-Based Hydrogels
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Cellulose (C104842), allyl glycidyl ether (A106314), and ammonium persulfate (A112447) were provided by Aladdin Reagent Co., Ltd. (Shanghai, China). Acrylic acid was a chemically pure reagent and was provided by Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Sodium hydroxide, ether, acetone, and urea were analytical reagents and were provided by Guangzhou Chemical Reagent Factory (Guangdong, China).
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