Nhs mpeg

All materials and solvents were used as received without further purification. Dulbecco’s modified Eagle’s medium (DMEM) (high glucose with _L-glutamine sterile-filtered) and fetal bovine serum (FBS) were purchased from Sigma-Aldrich. Hexadecyltrimethylammonium bromide (CTAB), _L-ascorbic acid (AA) (99.9%), silver nitrate (99%), gold(III) chloride trihydrate (99.9%), sodium oleate (NaOL, >95%), TiCl₃ (12%), poly(ethylene glycol) (PEG)-SH (2000), 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), phenazine methosulfate (PMS), N-hydroxysuccinimidyl–dibenzocyclooctyne (NHS–DBCO), dopamine, and 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA) were purchased from Sigma-Aldrich. Sodium bicarbonate (NaHCO₃, 99%), sodium borohydride (NaBH₄, 99%), and hydrochloric acid (HCl) (36.5–38% in water) were purchased from Fisher Scientific. The azide-ICG was bought from Intrace-medical. All of the chemicals were used as received without further purification. The DNA was purchased from Integrated DNA Technologies Inc. DOX hydrochloride was bought from Tokyo Chemical Industry Co. Ltd. NHS–mPEG was bought from Laysan Bio, Inc. Deionized water was used throughout the experiments.