4 4 6 dimethoxy 1 3 5 triazin 2 yl 4 methyl morpholinium chloride dmtmm

Dextran (40 kDa, pharmaceutical grade) was purchased from Pharmacosmos, Holbæk, Denmark. Sodium hyaluronate (27 kDa, pharmaceutical grade) was purchased from Contipro Pharma, Dolní Dobrouč, Czech Republic. Tyramine (99%), DMF (anhydrous, 99.8%), LiCl (99.0%), p-nitrophenyl chloroformate (PNC, 96%), pyridine (anhydrous, 99.8%), DMSO-d₆ (99.9%), NaCl (≥99.0%), D₂O (99.9 atom % D), horseradish peroxidase (HRP, 325 U/mg solid), and hydrogen peroxide (30%) were purchased from Sigma-Aldrich, Schnelldorf, Germany. Tyramine∙HCl salt (99%) was obtained from Acros Organics, Fair Lawn, NJ, USA. 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM, 97%) was purchased from Fluorochem Ltd., Hadfield, UK. Ethanol (≥99.9%) and diethyl ether (≥99.7%) were purchased from Merck, Kenilworth, NJ, USA. Milli-Q water was used from the Milli-Q Advantage A10 system (Merck KGaA, Darmstadt, Germany) equipped with a 0.22 μm Millipak^® 40 Express filter.

Dextran (40 kDa, pharmaceutical grade) was purchased from Pharmacosmos, Holbæk. Denmark. Sodium hyaluronate (27 kDa, pharmaceutical grade) was purchased from Contipro Pharma, Dolní Dobrouč, Czech Republic. Tyramine (99%), DMF (anhydrous, 99.8%), LiCl (99.0%), p-nitrophenyl chloroformate (PNC, 96%), pyridine (anhydrous, 99.8%), DMSO-d6 (99.9%), NaCl (≥99.0%), D₂O (99.9 atom % D), horseradish peroxidase (HRP, 325 units/mg solid) and hydrogen peroxide (30%) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Tyramine HCl salt (99%) was obtained from Acros Organics, Fair Lawn, NJ, USA. 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%) was purchased from Fluorochem Ltd., Hadfield, UK. Ethanol (≥99.9%) and diethyl ether (≥99.7%) were purchased from Merck, Kenilworth, NJ, USA. Milli-Q water was used from the Milli-Q Advantage A10 system equipped with a 0.22 μm Millipak^®-40 Express filter.

Poly(D,L-lactide-co-glycolide) RG502 Resomer (PLGA, acid terminated PLGA-COOH), Pluronic F127, biotin, cysteamine hydrochloride, 2-mercaptoethanol, sodium azide, d6-dimethylsulfoxide (d6-DMSO), and Spectra/Por^® dialysis membranes (MWCO 3.5 kDa) were purchased from SigmaAldrich (Merk Life Science, Milan, Italy). HEPES sodium salt and 2 M hydrochloric acid solution were supplied from Alfa Aesar (Thermo Fisher, Kandel, Germany). The 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was from Fluorochem (Hadfield, UK).