Mouse renal MC lines (SV40-MES13) were purchased from Shanghai Cell Bank of Chinese Academy of Science. SKF38393 (DR1 agonist), sodium hydrogen sulfide (NaHS), PPG (a CSE inhibitor), PD98059 (an ERK1/2 inhibitor), streptozocin (STZ) and 7-Azido-4-Methylcoumarin (H2S probe) were purchased from Sigma-Aldrich (Merck KGaA). The primary antibodies for anti-CSE, cyclin D1, PCNA, P21, COL1, α-smooth muscle actin (α-SMA), and MMP9 were from ProteinTech Group, Inc. DR1 antibody was purchased from GeneTex, Inc. The total (t)-ERK1/2 and phosphorylated (p)-ERK1/2 antibodies were obtained from Cell Signaling Technology, Inc. The Cell Counting Kit-8 (CCK-8) kit and the primary antibody for β-actin were obtained from Wuhan Boster Biological Technology, Ltd. The EdU kit was obtained from Guangzhou RiboBio Co., Ltd. The BCA Protein Assay kit and Enhanced Chemiluminescence (ECL) reagent were purchased from Beyotime Institute of Biotechnology.
Sv40 mes13
Manufactured by Shanghai Cell Bank
Sourced in United States
The SV40-MES13 is a cell line derived from mouse embryonic mesenchymal stem cells, which have been immortalized using the SV40 large T antigen. This cell line is commonly used in research applications to study mesenchymal stem cell biology and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence ecl reagent, by Beyotime (1 mentions)
Pd98059, by Merck Group (1 mentions)
Proliferating cell nuclear antigen (pcna), by Proteintech (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Streptozocin stz, by Merck Group (1 mentions)
α smooth muscle actin α sma, by Proteintech (1 mentions)
Anti cse, by Proteintech (1 mentions)
Cell counting kit 8 cck 8 kit, by Wuhan Boster Biological Technology (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using sv40 mes13
1
SV40-Immortalized Mouse Renal Mesangial Cells
2
Renal Mesangial Cell Glucose Regulation
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Mouse renal mesangial cells (SV40-MES13) were purchased from Shanghai Cell Bank of Chinese Academy of Science, and cultured in DMEM/F12 medium (Gibco, U.S.A.) containing 5% fetal bovine serum (FBS, Gibco, U.S.A.). For the low-glucose group, cells were stimulated with 5.5 mM D-glucose, together with 24.5 mM mannitol; and for the high-glucose group, cells were stimulated with 30 mM glucose. Cells were incubated in humidified 95% air with 5% carbon dioxide at 37°C.
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