Three different Candida strains were used including C. tropicalis T26, C. tropicalis U71 and C. tropicalis V89. All strains were clinical isolates and previously characterized as highly biofilm forming (Sahal and Bilkay, 2018) . Identification was done using the 18S Ribosomal RNA Gene Sequence Analysis (Ref Gen Biotechnology Co. Ltd., 2017; Ankara, Turkey).
All Candida strains were first grown on Brain Heart Infusion (BHI) agar (Lab M Ltd, Lancashire, UK) for 24 h at 37 °C. For pre-culture preparations, single colonies were inoculated into 10 mL BHI broth (Lab M Ltd, Lancashire, UK) and incubated overnight at 37 °C. This preculture was used to inoculate a second culture of 200 mL BHI broth, which was subsequently grown for 24 h at 37 °C. Cells were harvested by centrifugation at 3220g for 10 min at 5 °C (Eppendorf 5810R, with an Eppendorf Swing-bucket rotor A-4-62, Hamburg, Germany) and washed 3 times with 10 mM potassium phosphate buffer (pH 7). Cells were counted in a thoma counting chamber and diluted appropriately to obtain the cell concentrations required for the experiments.
All Candida strains were first grown on Brain Heart Infusion (BHI) agar (Lab M Ltd, Lancashire, UK) for 24 h at 37 °C. For pre-culture preparations, single colonies were inoculated into 10 mL BHI broth (Lab M Ltd, Lancashire, UK) and incubated overnight at 37 °C. This preculture was used to inoculate a second culture of 200 mL BHI broth, which was subsequently grown for 24 h at 37 °C. Cells were harvested by centrifugation at 3220g for 10 min at 5 °C (Eppendorf 5810R, with an Eppendorf Swing-bucket rotor A-4-62, Hamburg, Germany) and washed 3 times with 10 mM potassium phosphate buffer (pH 7). Cells were counted in a thoma counting chamber and diluted appropriately to obtain the cell concentrations required for the experiments.