FN-null MEFs were seeded at a density of 2 × 104 cells/cm2 onto collagen gels (2 mg/mL) polymerized under either US or sham exposure conditions. In some experiments, cells were preincubated with 25 μg/mL of the following antibodies before seeding: anti-β1 integrin (clone Ha2/5), anti-β3 integrin (clone 2C9.G2), or IgG and IgM isotype controls (BD Biosciences). Cell-seeded collagen gels were incubated for 20 min at 37 °C and 8% CO2 and then washed with PBS to remove non-adherent cells. Collagen gel surfaces were subsequently imaged using phase-contrast microscopy. Two regions of interest (2.2 mm × 1.7 mm) were selected from each gel, one located at the center of the US-exposed area and one located outside the beam area (4 mm off-center). The number of adherent cells within each region of interest was quantified using FIJI software (NIH).
Anti β1 integrin clone ha2 5
Manufactured by BD
Anti-β1 integrin (clone Ha2/5) is a monoclonal antibody that binds to the β1 subunit of the integrin receptor. Integrins are cell surface receptors that mediate cell-cell and cell-extracellular matrix interactions.
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2 protocols using anti β1 integrin clone ha2 5
1
Integrin-Mediated Cell Adhesion on Ultrasound-Exposed Collagen Gels
2
Integrin-Mediated Cell Adhesion on Ultrasound-Exposed Collagen Gels
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FN-null MEFs were seeded at a density of 2 × 104 cells/cm2 onto collagen gels (2 mg/mL) polymerized under either US or sham exposure conditions. In some experiments, cells were preincubated with 25 μg/mL of the following antibodies before seeding: anti-β1 integrin (clone Ha2/5), anti-β3 integrin (clone 2C9.G2), or IgG and IgM isotype controls (BD Biosciences). Cell-seeded collagen gels were incubated for 20 min at 37 °C and 8% CO2 and then washed with PBS to remove non-adherent cells. Collagen gel surfaces were subsequently imaged using phase-contrast microscopy. Two regions of interest (2.2 mm × 1.7 mm) were selected from each gel, one located at the center of the US-exposed area and one located outside the beam area (4 mm off-center). The number of adherent cells within each region of interest was quantified using FIJI software (NIH).
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