Chemicals were obtained from the following sources: manganese standard for Atomic Absorption Spectrometry (AAS) from Fluka; arsenic standard solution for AAS (H3AsO4), nitric acid 65% suprapure (HNO3), delta-aminolevulinic acid standard from Merck; lead acetate trihydrate puriss. [Pb (CH3CO2)2•3H2O], lead for AAS standard solution, manganese chloride tetrahydrate (MnCl2•4H2O; 99.99%), sodium (meta)arsenite purum (AsO2Na; ≥ 99%), 2-Propanol for HPLC (C3H8O; 99.5%), 5,5 –dithiobis (2-nitrobenzoic acid) [DTNB], acetylthiocholine (ATCh), diethyl ether (C4H10O), ethopropazine, ethyl acetate (C4H8O2), ethyl acetoacetate p.a. (C6H10O3), methanol HPLC grade (CH4O; ≥99.9%), sodium phosphate dibasic (Na2HPO4), sodium phosphate monobasic (NaH2PO4) from Sigma–Aldrich. Pure standards of porphyrins (10 nM) were obtained from Porphyrin Products, Frontier Scientific.
δ aminolevulinic acid
Manufactured by Merck Group
Sourced in United States
δ-aminolevulinic acid is a chemical compound used in various laboratory applications. It is a precursor in the biosynthesis of heme, chlorophyll, and other tetrapyrrolic compounds. The compound can be utilized in biochemical and analytical procedures that require the study of these biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Chloramphenicol, by Merck Group (5 mentions)
Ampicillin, by Merck Group (5 mentions)
Kanamycin sulfate, by Merck Group (3 mentions)
Resorufin, by Merck Group (3 mentions)
Thiamine, by Merck Group (3 mentions)
Fluorescein, by Merck Group (3 mentions)
Bacto tryptone, by BD (3 mentions)
Bacto yeast extract, by Formedium (3 mentions)
L arginine, by Merck Group (3 mentions)
Glucose 6 phosphate (g6p), by Merck Group (3 mentions)
Glucose 6 phosphate dehydrogenase, by Merck Group (3 mentions)
Bacto peptone, by BD (3 mentions)
Lb broth, by BD (3 mentions)
Formic acid, by Thermo Fisher Scientific (3 mentions)
Dibenzylfluorescein, by Santa Cruz Biotechnology (2 mentions)
Polyclonal antibody from rabbit serum raised against recombinant human cpr, by GeneTex (2 mentions)
Egg lysozyme, by Merck Group (2 mentions)
Restriction enzyme, by New England Biolabs (2 mentions)
Imidazole, by Merck Group (2 mentions)
Sodium hydrosulfite na2s2o4, by Merck Group (2 mentions)
14 protocols using δ aminolevulinic acid
1
Trace Metal Analysis in Biological Samples
2
Analytical Methods for Trace Elements
3
Recombinant Expression of SaBphP1 Domains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The coding region for residues 1–328 (SaBphP1-CBD) and residues 1–516 (SaBphP1-PCM) of the wild-type SaBphP1 were PCR-amplified from S. aurantiaca DW4/3–1 genomic DNA and cut by restriction enzymes NdeI and HindIII (New England Biolabs, Beverly, USA), and ligated into the corresponding sites of the expression vector pET28c(+) (Invitrogen, Carlsbad, CA). The following primers 5′-CACCAGCATATGAGCACTGAGGCGTCCCGGAGC-3′ (forward) and 5′-CCAAAGCTTAGCGCTGGTCATAGTCCTCGT-3′ (reverse), and 5′-CCAAAGCTTAGAGCAGTTCCTCGCTGCGCT-3′ (reverse) were used to PCR-amplify the coding region of SaBphP1-CBD and SaBphP1-PCM, respectively. Site-directed mutagenesis to prepare the Thr289His (T289H) mutant and all other mutants described in this manuscript was carried out using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, USA). The constructed plasmids and the pET11a vector carrying heme oxygenase were transformed into Escherichia coli BL21 (DE3) strain for expression. Cells were grown aerobically at 310 K to 3 × 108 cells ml−1 followed by induction with 1 mM isopropyl-β-d -thiogalactopyranoside and addition of 0.5 mM δ-aminolevulinic acid overnight (Sigma-Aldrich, St Louis, USA). Proteins were purified as previously described (Yang et al., 2007 ▸ ). All steps were performed under green safety light.
4
Biochemical Modulators of Glycosylation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucosamine, MG132, OGT inhibitors (DON and azaserine), the OGA inhibitor Thiamet G, δ-aminolevulinic acid, and succinyl acetone were purchased from Sigma-Aldrich. Compounds were used at indicated concentrations (see figure legends).
5
Recombinant Human CPR Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
L -Arginine, thiamine, chloramphenicol, ampicillin, kanamycin sulfate, isopropyl β-D -thiogalactoside (IPTG) (dioxane-free), δ-aminolevulinic acid, glucose 6-phosphate, glucose 6-phosphate dehydrogenase, nicotinamide adenine dinucleotide phosphate (NADP+ and NADPH) were obtained from Sigma-Aldrich (St. Louis, MO, United States). LB Broth, bacto tryptone and bacto peptone were purchased from BD Biosciences (San Jose, CA, United States). Bacto yeast extract was obtained from Formedium (Norwich, United Kingdom). EthoxyResorufin, mEthoxyResorufine and coumarin were obtained from BD Biosciences (San Jose, CA, United States) and dibenzylfluorescein from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Resorufin, 7-hydroxy coumarin, fluorescein and dichlorophenolindophenol (DCPIP) were obtained from Sigma-Aldrich (St. Louis, MO, United States). A polyclonal antibody from rabbit serum raised against recombinant human CPR obtained from Genetex (Irvine, CA, United States) was used for immune-detection of the membrane-bound CPR. All other chemicals and solvents were of the highest grade commercially available.
+ Expand
6
Purification and Analysis of Recombinant Proteins
7
Synthesis of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alpha- and beta-ionone, steroids, and monoterpenoids were purchased from Tokyo Chemical Industry Co., Ltd. (Seoul, Korea). All of the flavonoids used in this study, δ-aminolevulinic acid (ALA), formate dehydrogenase, ampicillin, and NADH were purchased from Sigma-Aldrich (Yongin, Korea). Isopropyl-1-thio-β-D-galactopyranoside (IPTG), 1, 4-dithiothreitol (DTT), and kanamycin were purchased from Duchefa Biochemie (Haarlem, The Netherlands)). Restriction enzymes, T4 DNA ligase, dNTPs, and DNA polymerase were purchased from Takara Bio (Shiga, Japan). All other high-grade chemicals were purchased from available commercial sources.
8
Determining Blood Lead Levels and Antioxidant Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The kit used to determine the Pb levels from the whole blood samples was (BH 2100 kit). It was purchased from Beijing Bohui Innovation Technology Co., Ltd. All other kits were acquired from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). These kits include Njjcbio C009 which was used to determine ALT (alanine aminotransferase), and Njjcbio C010 was used to determine AST (aspartate aminotransferase). Similarly, Njjcbio A001 and Njjcbio A003 kits were used to determine SOD (superoxide dismutase) and MDH (malondialdehyde), respectively. Likewise, Njjcbio A005 was used to determine the GSH–Px (GSH peroxidase), and Njjcbio A006 was used for GSH (glutathione). And Njjcbio A007 kit was used to determine CAT (catalase) activity. δ-Aminolevulinic acid and maize-resistant starch (CAS: 9005-25-8) were procured from Sigma Aldrich (Mainland, China). Lead nitrate and all other reagents used in the experiments were of analytical grade and procured from Jinan Boss Chemical Company (Jinan, China).
9
Purification of Heme-containing Proteins
10
CYP2C19 Expression and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
QuickChange® Site-Directed Mutagenesis system was obtained from Stratagene (CA, USA). Bovine serum albumin was acquired from Promega (WI, USA). β-Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt (NADPH), omeprazole hydrochloride, 5-hydroxyomeprazole, ketoconazole, fluoxetine hydrochloride, CEC, 3cyanoumbelliferone (CHC), magnesium chloride (MgCl 2 ), potassium phosphate monobasic, potassium phosphate dibasic, Terrific broth, sodium dithionite, δ-aminolevulinic acid, isopropyl β-D-1-thiogalactopyranoside, phenylmethanesulfonyl fluoride, protease inhibitor, sucrose, potassium phosphate monobasic and dibasic, dimethyl sulfoxide (DMSO), and ethylenediaminetetraacetic acid were obtained from Sigma (MO, USA). Sertraline hydrochloride and loratadine hydrochloride were acquired from Toronto Research Chemicals Inc., Canada. All HPLC solvents were obtained from Fisher Scientific (USA). Supelco Ascentis C18 column was purchased from Sigma-Aldrich (MO, USA); and 96-well plates were obtained from Perkin Elmer (USA). CYP2C19 cDNA was successfully cloned via reverse transcription-polymerase chain reaction (RT-PCR) which was reported in our previous work [21] (link). The cDNA was inserted into pCWori+, a commonly used bacterial plasmid for CYP expression.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!