The morphologies of the MoS2 nanosheets modified by ethanethiol and nonanethiol were observed using a Tecnai™ G2 F-20 (Philips, Amsterdam, Netherlands) transmission electron microscope (TEM). Raman spectra and Raman maps were obtained using an NRS5100 (JASCO, Tokyo, Japan) spectrometer. Cross-sectional images were obtained using a JSM-6500F (JEOL, Tokyo, Japan) scanning electron microscope (SEM); and, composite samples were cooled in liquid nitrogen and cut by a scalpel to prepare the samples for backscattered electron (BSE) imaging. Atomic force microscopy (AFM) was performed using a NX10 system (Park, Suwon, Korea). X-ray diffraction (XRD) was performed using a D8 SSS (Bruker, Billerica, MA, USA). UV-Vis spectra were obtained using a V-730 spectrometer (JASCO, Tokyo, Japan). Dynamic mechanical analysis was performed using a Q800 (TA Instruments, New Castle, DE, USA), while stress-strain curves were measured using a TS-2000 with a crosshead speed of 500 mm/min. The oxygen transmission rates were measured according to the ASTM D3985 standard using the OX-TRAN 2/61 (Mocon Inc., Minneapolis, MN, USA) at 23 °C and a relative humidity of 0%; film specimens of 5 cm in diameter and 1 mm in thickness were fixed between two chambers, and oxygen filled the upper chamber while nitrogen filled the lower chamber.
V 730 spectrometer
Manufactured by Jasco
Sourced in Japan
The V-730 spectrometer is a laboratory instrument designed for spectroscopic analysis. It measures the absorption or transmission of light through a sample across a range of wavelengths. The core function of the V-730 is to provide quantitative and qualitative data about the chemical composition and properties of materials.
Automatically generated - may contain errors
Lab products found in correlation
D8 sss, by Bruker (1 mentions)
Tecnai g2 f20, by Philips (1 mentions)
Jsm 6500f, by JEOL (1 mentions)
Nrs 5100, by Jasco (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Ldh d c 405, by PicoQuant (1 mentions)
Hydraharp400 module, by PicoQuant (1 mentions)
Microtime 200, by PicoQuant (1 mentions)
10 protocols using v 730 spectrometer
1
Characterizing MoS2 Nanosheet Morphologies
2
AuNRs Characterization by UV-Vis-NIR and Zeta
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UV/vis/NIR absorption spectra were measured with a JASCO V-730 spectrometer (Tokyo, Japan). The zeta potential of all AuNRs was measured in 10 mM Tris-HCl (pH 7.0) with a Malvern Zetasizer Nano Z (Worcestershire, UK).
3
UV/Vis/NIR Absorption Spectroscopy
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Absorption measurements in the UV/Vis/NIR region of the spectrum were carried out with a V-730 spectrometer (JASCO, Japan) at T ~25 °C, using quartz cuvettes S15-UV-1 (GL Sciences Inc., Japan) with an optical path length of L = 0.1 cm23 (link),25 (link),30 (link).
4
UV-Visible Absorption Spectroscopy of Compounds
5
Determining Molar Extinction Coefficients
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UV-vis absorption spectra were recorded using a Jasco v730 spectrometer with 5 mm path length quartz cuvettes. Molar extinction coefficients (ε) were calculated at local peak maxima by regressing absorption versus concentration for five concentrations.
6
Optical characterization of nanoparticles
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All optical measurements were carried out at room temperature. Absorption/extinction measurements were performed on a UV-Vis Jasco V-730 spectrometer. Photoluminescence (PL) spectra were collected with a spectrometer Edinburgh Instruments FCS900 mounting both a xenon lamp and a pulsed diode laser – VIS/NIR 375 nm, from the same company. The latter was used to carry out lifetime measurements on a population of nanoparticles in solution. For single molecule measurements, NPLs were diluted in hexane to a concentration of 0.02 nmol L−1 and deposited by drop-casting on a glass substrate. Single NCs were studied on a commercial confocal microscope (Microtime 200, Picoquant) associated with a pulsed laser diode emitting at 405 nm (LDH-D-C-405, Picoquant). The fluorescence was collected through an oil-objective (NA 1.4, 100×, Olympus) and sent into a Hanbury-Brown and Twiss acquisition setup (SPAD PDM; time resolution: 50 ps). The signal was recorded using a HydraHarp 400 module (Picoquant) in a time-tagged, time-resolved mode. Transmission electron microscopy (TEM) images were acquired on a JEOL 2010 field electron gun microscope operated at 200 keV. Scanning electron microscopy (SEM) images were taken using an FEI Magellan.
7
UV-Vis Spectroscopic Characterization
8
Liposomal Formulation Spectroscopic Analysis
9
Spectroscopic Analysis of Coumarin Derivatives
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The absorption properties (UV-visible absorption spectrum and molar extinction coefficient) as well as the steady state photolysis of the investigated Coumarin derivatives (CoumA–CoumI) in acetonitrile have been investigated using a JASCO V730 spectrometer.
10
Reversible DNA Nanostructure Regulation
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Strand 3 was labeled with FAM. The samples were prepared by mixing the DNA strands (2 µM each) in 10 mM HEPES buffer (pH 8.0) containing 100 mM NaCl. The solutions were annealed (85 °C → 4 °C, −1.0 °C/min) in the absence or presence of GdIII ions. For the reversible regulation shown in Fig. 2f , after the solutions were annealed (85 °C → 25 °C, −1.0 °C/min), GdCl3 (4 equiv) and EDTA (4 equiv) were alternately added every 2 h at 25 °C. Native gel electrophoresis was carried out with 20% polyacrylamide gel in a cool incubator (4 °C). The bands were detected by FAM fluorescence, and the yield of each product was calculated by comparing the band intensities of the authentic samples on the same gel. The averages of at least three runs are summarized. The gel shown in Supplementary Fig. 5 was stained with SYBR Gold. The UV spectra of the samples were also recorded at 5 °C on a JASCO V-730 spectrometer.
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