At 2-week intervals over 2 months following the ovariectomy or sham operations (time-points of 2, 4, 6 and 8 weeks), each rat was anesthetized with pentobarbital at a dose of 40 mg/kg during µCT scanning. The American Society for Bone and Mineral Research recommendations for small-animal µCT (22 (link)) were followed during the process of µCT analysis. A pre-clinical Inveon µCT system (Siemens Healthineers) with a resolution of 8 mm, tube current of 0.1 mA and tube voltage of 50 kV was used to scan distal femurs. The three-dimensional quantitative analyses were performed using the µCT system (Inveon Research Workplace 2.2; Siemens Healthineers). Scanning regions were confined from the distal metaphysis and extended 2.0 mm proximally from the proximal tip of the primary spongiosa. The following three-dimensional indices in the defined region of interest were determined: BMD, trabecular thickness (Tb.Th) and relative bone volume over the total volume (BV/TV, %). The examiner performing the scan analyses was blinded to the experimental group of the subjects.
Inveon research workplace 2
Manufactured by Siemens
Sourced in Germany
The Inveon Research Workplace 2.2 is a multi-modality preclinical imaging system designed for small animal research. It provides simultaneous acquisition of positron emission tomography (PET), single-photon emission computed tomography (SPECT), and computed tomography (CT) data. The system is capable of high-resolution imaging and quantitative analysis for a wide range of preclinical applications.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using inveon research workplace 2
1
Evaluating Bone Changes Following Ovariectomy
2
Femoral Bone Microarchitecture Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rats were anesthetized with 3% sodium pentobarbitone allowing the microarchitecture of the femoral cavities to be examined by micro-CT. Bone images were reconstructed using an isotropic voxel size of 10 μm. Bone volume/total volume, trabecular thickness, trabecular number, and trabecular spacing values were recorded to evaluate bone formation. Three-dimensional imaging and the reconstruction of the microarchitecture were performed using an Inveon Research Workplace 2.2 (SIEMENS Healthineers, Berlin, Germany).
3
Microstructural Analysis of Peri-Implant Bone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bones containing implants were dissected, fixed in 4% paraformaldehyde and scanned by micro-CT (Inveon Research Workplace 2.2, Siemens Inc., Germany) at a resolution of 19.64 μm with an X-ray voltage of 80 kV and a current of 50 μA. The scanner software (Inveon Acquisition Workplace 2.2, Siemens Inc., Germany) was applied for image reconstruction and analyses. The bone around the implant with 0.5 mm thickness and 5.5 mm length (including 5 mm length of implant and 0.5 mm length of bone under the implant) was chosen as the region of interest (ROI) in each sample for microstructure analyses26 (link) (Fig. 2a ), and parameters such as trabecular bone volume/total volume (Tb.BV/TV, %), trabecular number (Tb.N, mm−1), trabecular thickness (Tb.Th, mm), trabecular separation (Tb.Sp, mm) and bone mineral density (BMD, mg/cc) were measured.![]()
Results of Micro-CT scan and analyses. (
4
Micro-CT Analysis of Bone Morphology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CKIP-1−/− and WT mice were sacrificed by excessive sodium pentobarbital (150 mg/kg; Sigma-Aldrich; Merck KGaA), and subsequently the femurs were isolated and fixed with 4% paraformaldehyde (PFA; FeiYang Biotech Co., Ltd.) for 24 h at room temperature. Micro-CT (YXLON Cheetah; YXLON International GmbH; 90 kV, 45 µA, 1,000 msec) was performed to scan the fixed femora with a layer thickness of 8 µm. Cancellous bone at the distal end of the femur and cortical bone in the middle part of the femur were selected as the area of interest for three-dimensional (3D) reconstruction. Bone volume/total volume ratio (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), bone surface/bone volume (BS/BV), trabecular separation (Tb.Sp), cortical area (Ct.Ar), cortical inner diameter perimeter (Ct.ld.Pm), cortical outer diameter perimeter (Ct.Od.Pm), cortical thickness (Ct.Th), cortical bone volume (Ct.BV), trabecular area (Tb.Ar) and trabecular width (Tb.Wi) were selected as measurement indices, and quantified by Inveon™ Research Workplace 2.2 (Siemens AG).
5
Micro-CT Analysis of Bone-Implant Interface
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The implants with surrounding bones were cut out and fixed in 4% paraformaldehyde. The specimens were scanned using micro-CT (Inveon Research Workplace 2.2, Siemens, Germany) at a resolution of 39.28 µm, with 80 kV voltage and 50 uA current. The bone-implant structure was reconstructed and analysed by the Inveon Acquisition Workplace 2.2 scanner program (Siemens, Germany). A 0.5-mm area around the implant was chosen as a region of interest (ROI) for longitudinal analysis of the peri-implant bone microstructure. Parameters related to bone volume/total volume (BV/TV, %) and trabecular separation (Tb.Sp, mm) were calculated to evaluate osseointegration and the extent of bone damage after impact loading.
After evaluation with Micro-CT, samples were prepared for histological study. The samples were dehydrated in a graded alcohol series, embedded in methyl methacrylate, and cut into longitudinal sections of 200-µm thickness. The sections were ground to 20 µm and stained with Van Gieson (VG). The images were captured with a microscope (DMI6000, Leica, Germany) and the bone-to-implant contact (BIC) was calculated to evaluate the extent of osseointegration and damage. Micro-CT and histological data were used for subsequent verification of the numerical simulation analysis.
After evaluation with Micro-CT, samples were prepared for histological study. The samples were dehydrated in a graded alcohol series, embedded in methyl methacrylate, and cut into longitudinal sections of 200-µm thickness. The sections were ground to 20 µm and stained with Van Gieson (VG). The images were captured with a microscope (DMI6000, Leica, Germany) and the bone-to-implant contact (BIC) was calculated to evaluate the extent of osseointegration and damage. Micro-CT and histological data were used for subsequent verification of the numerical simulation analysis.
6
Melatonin-Enhanced Bone Regeneration by BMMSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sixteen 8-week-old SD rats were randomly and equally distributed into four groups (P1, P4, P15 and P15+mel). Under general anesthesia, parts of bone at the central of calvarium were removed to form a roundness critical defect of 8 mm in diameter (thickness of the skull≈0.5 mm, volume≈25.13 mm3). Four layers of BMMSCs sheets were composited with three layers of HA/TCP (50 mg in total) to create a sandwich structure for calvarial defect repair. BMMSCs treated with melatonin from the 1st to the 15th passage was used in P15+mel group. BMMSCs treated with equal volume of DMSO (as vehicle control) for 1, 4, and 15 passages were used in P1, P4 and P15 group. All rats were sacrificed 12 weeks after transplantation and the skulls were fixed in 4% paraformaldehyde.
Reparative effect of BMMSCs was analyzed by micro-CT (Siemens Inveon, Eschborn, Germany) in terms of the scanning protocol (voltage of 80 kV, current of 500 mA and isotropic resolution of 14.97 mm). New bone formation was analyzed using Inveon Research Workplace 2.2 (Siemens). After micro-CT scanning, all skulls were decalcified by 17% EDTA, embedded with paraffin and sliced in the coronal plane for hematoxylin and eosin (H&E) staining. The regenerative junctions were photographed under a light and polarized microscopy (Olympus).
Reparative effect of BMMSCs was analyzed by micro-CT (Siemens Inveon, Eschborn, Germany) in terms of the scanning protocol (voltage of 80 kV, current of 500 mA and isotropic resolution of 14.97 mm). New bone formation was analyzed using Inveon Research Workplace 2.2 (Siemens). After micro-CT scanning, all skulls were decalcified by 17% EDTA, embedded with paraffin and sliced in the coronal plane for hematoxylin and eosin (H&E) staining. The regenerative junctions were photographed under a light and polarized microscopy (Olympus).
7
Intravital Micro-CT Analysis of Trabecular Bone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 2 months maintenance in a SPF room, mice in the OVX and sham groups, or mimic BMMSCs, inhibitor BMMSCs and control BMMSCs groups were anaesthetized by intraperitoneal injection of pentobarbital sodium and placed on Micro-CT apparatus for an intravital scan of the proximal femoral area. The micro-CT scan was performed using a scanning angle of 360° and a resolution of 9 µm along the long axis of the femur. Following the Micro-CT scan, an area of 1 mm below the growth plate and 1.5 mm from the distal femur was selected as the range of interest and a three-dimensional image of the trabecular bone was reconstructed. Finally, parameters representing the spatial structure of trabecular bone, including bone volume relative to tissue volume (BV/TV;%), trabecular thickness (Tb.Th; µm), trabecular spacing (Tb.Sp; mm) and trabecula number (Tb.N; 1/mm) were analyzed and determined using the built-in software (Inveon Research Workplace 2.2, Siemens Healthineers, Erlangen, Germany).
8
Micro-CT Analysis of Femur Microarchitecture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized with 5% chloral hydrate and positioned for micro-CT scanning of the microarchitecture of whole femurs. The images were reconstructed to an isotropic voxel size of 10 μm. Bone volume/total volume, bone surface area/bone volume, trabecular thickness, trabecular number, and trabecular spacing were calculated and three-dimensional image manipulations were performed by the system software (Inveon Research Workplace 2.2, SIEMENS Healthineers).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!