Alexa fluor 680 coupled goat anti mouse igg

Manufactured by Thermo Fisher Scientific

Alexa Fluor 680–coupled goat anti-mouse IgG is a fluorescent secondary antibody used to detect and visualize mouse primary antibodies in various immunoassays and imaging applications. It is conjugated with the Alexa Fluor 680 dye, which has an excitation maximum of 679 nm and an emission maximum of 702 nm.

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Lab products found in correlation

Nitrocellulose coated glass slides, by Grace Bio-Labs (2 mentions)

Spelling variants of this product

Alexa Fluor 680 (241 citations) Alexa Fluor 680 goat anti-mouse IgG (42 citations) Alexa Fluor 680 goat anti-mouse (39 citations) Alexa 680 (31 citations) Alexa Fluor 680 anti-mouse IgG (14 citations) Alexa Fluor goat anti-mouse IgG (10 citations) Anti-mouse alexa fluor 680 (10 citations) Goat anti-mouse IgG Alexa 680 (4 citations) Anti-mouse Alexa Fluor (4 citations) Mouse anti-IgG (2 citations)

2 protocols using alexa fluor 680 coupled goat anti mouse igg

Quantitative Protein Profiling Assay

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Lysates were adjusted to a total protein concentration of 2 μg/μL, mixed with 4 × SDS sample buffer and denatured at 95°C. Lysates and dilution series of each cell line were spotted as technical triplicates on nitrocellulose-coated glass slides (Grace-Biolabs). Post spotting, slides were incubated with the blocking buffer containing 5 mmol/L NaF and 1 mmol/L Na₃VO₄, prior to overnight incubation with primary antibodies at 4°C. Primary antibodies were detected using Alexa Fluor 680–coupled goat anti-mouse IgG (Life Technologies). In addition, representative slides were stained for total protein quantification using Fast Green FCF protein dye. Signal intensities were quantified using GenePixPro 7.0 (Molecular Service). Data preprocessing, merging of technical triplicates, and background correction were performed using the RPPanalyzer Rpackage.

Noronha A., Belugali Nataraj N., Lee J.S., Zhitomirsky B., Oren Y., Oster S., Lindzen M., Mukherjee S., Will R., Ghosh S., Simoni-Nieves A., Verma A., Chatterjee R., Borgoni S., Robinson W., Sinha S., Brandis A., Kerr D.L., Wu W., Sekar A., Giri S., Chung Y., Drago-Garcia D., Danysh B.P., Lauriola M., Fiorentino M., Ardizzoni A., Oren M., Blakely C.M., Ezike J., Wiemann S., Parida L., Bivona T.G., Aqeilan R.I., Brugge J.S., Regev A., Getz G., Ruppin E, & Yarden Y. (2022). AXL and Error-Prone DNA Replication Confer Drug Resistance and Offer Strategies to Treat EGFR-Mutant Lung Cancer. Cancer Discovery, 12(11), 2666-2683.

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Quantitative Protein Profiling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lysates were adjusted to a total protein concentration of 2 μg/μl, mixed with 4×SDS sample buffer and denatured at 95°C. Lysates and dilution series of each cell line were spotted as technical triplicates on nitrocellulose-coated glass slides (Grace-Biolabs, Bend, OR). Post spotting, slides were incubated with the blocking buffer containing 5 mM NaF and 1 mM Na₃VO₄, prior to overnight incubation with primary antibodies at 4°C. Primary antibodies were detected using Alexa Fluor 680-coupled goat anti-mouse IgG (Life Technologies, Darmstadt, Germany). In addition, representative slides were stained for total protein quantification using Fast Green FCF protein dye. Signal intensities were quantified using GenePixPro 7.0 (Molecular Service, Sunnyvale, CA). Data preprocessing, merging of technical triplicates and background correction were performed using the RPPanalyzer Rpackage.

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