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Cyclin d1

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Cyclin-D1 is a protein that plays a crucial role in the regulation of the cell cycle. It is a key component of the cell cycle machinery and is involved in the transition from the G1 phase to the S phase of the cell cycle. Cyclin-D1 is an important biomarker and can be used to study cell proliferation and cell cycle progression.

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Lab products found in correlation

Bz 9000 microscope, by Keyence (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Cyclin d1, by Abcam (2 mentions) Synaptopodin p 19, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 549, by Dianova (2 mentions) Lotus tetragonolobus lectin ltl fitc, by Vector Laboratories (2 mentions) Alexa fluor 647, by Dianova (2 mentions) Bz 2 analyzing software, by Keyence (2 mentions) Alexa fluor 488, by Dianova (2 mentions)

2 protocols using cyclin d1

1

Immunofluorescent Analysis of Kidney Markers

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Immunofluorescence staining was performed on 2-μm paraffin-embedded sections. CD44 (R&D Systems, Minneapolis, MN), synaptopodin (P-19; Santa Cruz Biotechnology, Dallas, TX), cyclin-D1 (ab16663; Abcam, Cambridge, UK), Ki-67 (ab16667–500; Abcam), SSeCKS (homemade by Dr. Gelman), K19 (ab15463; Abcam), K7 (ab9021; Abcam), and EGFP (ab13970; Abcam) immunostaining were performed as described previously.12 (link) The following secondary antibodies were used: donkey anti-rabbit, mouse, chicken, or rat Alexa Fluor 488, Alexa Fluor 549, or Alexa Fluor 647 (Dianova, Hamburg, Germany). The nuclei were stained using Hoechst 33342 (Sigma-Aldrich, St. Louis, MO). Sections were evaluated with a Keyence BZ-9000 Microscope using BZ-II Analyzing software (Keyence Corporation, Osaka, Japan). Analysis of distributions of EGFP, cyclin-D1, and Ki-67 on Bowman’s capsule was performed on sections counterstained with Hoechst and Fluorescein-labeled Lotus Tetragonolobus Lectin (LTL-FITC; Vector Laboratories, Burlingame, CA). At least in 25 glomeruli per kidney section, the location in relation to LTL-FITC of EGFP/cyclin-D1/Ki-67 was analyzed.
Kuppe C., Leuchtle K., Wagner A., Kabgani N., Saritas T., Puelles V.G., Smeets B., Hakroush S., van der Vlag J., Boor P., Schiffer M., Gröne H.J., Fogo A., Floege J, & Moeller M.J. (2019). Novel parietal epithelial cell subpopulations contribute to focal segmental glomerulosclerosis and glomerular tip lesions. Kidney international, 96(1), 80-93.
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2

Immunofluorescent Analysis of Kidney Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed on 2-μm paraffin-embedded sections. CD44 (R&D Systems, Minneapolis, MN), synaptopodin (P-19; Santa Cruz Biotechnology, Dallas, TX), cyclin-D1 (ab16663; Abcam, Cambridge, UK), Ki-67 (ab16667–500; Abcam), SSeCKS (homemade by Dr. Gelman), K19 (ab15463; Abcam), K7 (ab9021; Abcam), and EGFP (ab13970; Abcam) immunostaining were performed as described previously.12 (link) The following secondary antibodies were used: donkey anti-rabbit, mouse, chicken, or rat Alexa Fluor 488, Alexa Fluor 549, or Alexa Fluor 647 (Dianova, Hamburg, Germany). The nuclei were stained using Hoechst 33342 (Sigma-Aldrich, St. Louis, MO). Sections were evaluated with a Keyence BZ-9000 Microscope using BZ-II Analyzing software (Keyence Corporation, Osaka, Japan). Analysis of distributions of EGFP, cyclin-D1, and Ki-67 on Bowman’s capsule was performed on sections counterstained with Hoechst and Fluorescein-labeled Lotus Tetragonolobus Lectin (LTL-FITC; Vector Laboratories, Burlingame, CA). At least in 25 glomeruli per kidney section, the location in relation to LTL-FITC of EGFP/cyclin-D1/Ki-67 was analyzed.
Kuppe C., Leuchtle K., Wagner A., Kabgani N., Saritas T., Puelles V.G., Smeets B., Hakroush S., van der Vlag J., Boor P., Schiffer M., Gröne H.J., Fogo A., Floege J, & Moeller M.J. (2019). Novel parietal epithelial cell subpopulations contribute to focal segmental glomerulosclerosis and glomerular tip lesions. Kidney international, 96(1), 80-93.
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