Automacs pro machine

Manufactured by Miltenyi Biotec

Sourced in Germany

The AutoMACS Pro is a magnetic cell separation system designed for automated, high-throughput cell isolation. It allows for the rapid and efficient separation of target cells from complex sample mixtures using magnetic bead-conjugated antibodies. The system's core function is to provide a streamlined and reproducible cell separation process, optimizing the recovery and purity of the desired cell population.

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Lab products found in correlation

Red blood cell lysis buffer, by Thermo Fisher Scientific (2 mentions) Facsaria cell sorter, by BD (1 mentions) 40 m strainer, by BD (1 mentions) 40 μm strainer, by BD (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Collagenase d, by Roche (1 mentions) Cpg odn 1826, by InvivoGen (1 mentions) Hiseq 4000, by Illumina (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Dnase 1, by Qiagen (1 mentions) Novaseq, by Illumina (1 mentions) Cd34 magnetic beads, by Miltenyi Biotec (1 mentions) Ficoll hypaque density centrifugation, by GE Healthcare (1 mentions)

Close spelling variants of this product

AutoMACS (460 citations) AutoMACS Pro (141 citations) AutoMACS machine (7 citations)

3 protocols using automacs pro machine

Splenic T Cell Isolation Protocol

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Splenic single cell suspensions were passed through a 40µm strainer (BD Falcon) and lysed with red blood cell lysis buffer (Life Technologies, CA). Isolation of naïve and total CD4⁺ T cells, total T cells/APCs was accomplished using magnetic beads and the AutoMACS Pro machine (Miltenyi Biotec) or manual magnets (EasySep Stemcell; Invitrogen). Flow sorting was done on Sony SH800Z or BD FACS Aria cell sorter.

Verghese D., Demir M., Chun N., Fribourg M., Cravedi P., Llaudo I., Woodruff T.M., Yadav P., Lira S.A., Medof M.E, & Heeger P.S. (2018). T cell expression of C5a receptor 2 augments murine regulatory T cell generation and regulatory-T-cell-dependent cardiac allograft survival. Journal of immunology (Baltimore, Md. : 1950), 200(6), 2186-2198.

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Isolation and Activation of Murine Splenic DCs

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Spleen was passed through a 40μm strainer (BD Falcon) and lysed with red blood cell lysis buffer (Life Technologies, CA). T cell depletion from bone marrow suspensions and isolation of murine splenic naïve CD4⁺ T cells, total T cells/APCs were accomplished using magnetic beads and the AutoMACS Pro machine (Miltenyi Biotec, Auburn, CA). For splenic DC isolation, single cell suspensions of spleen cells were treated with Collagenase D (Roche) for 30 minutes at 37°C and incubated with CD11c microbeads kits (Miltenyi Biotec) following the manufacturer’s protocol. The purity of DCs after isolation was 85–95%. Isolated DCs were stimulated with CpG ODN 1826 or LPS (both from InvivoGen) for 18 hours in 96 wells if needed.

Sheen J.H., Strainic M., Lui J., Zhang W., Yi Z., Medof M.E, & Heeger P.S. (2017). Toll like receptor-induced murine dendritic cell activation requires dendritic cell-intrinsic complement. Journal of immunology (Baltimore, Md. : 1950), 199(1), 278-291.

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Isolation and RNA-seq of human CD34+ HSPCs

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BM mononuclear cells (MNCs) were purified using Ficoll-Hypaque density centrifugation (GE Healthcare, Dornstadt, Germany). MNCs were incubated with CD34⁺ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) and separated using an AutoMACS Pro machine (Miltenyi Biotec) according to the manufacturer’s recommendations. This total (further unsorted) CD34⁺ cell population (referred to as CD34⁺ hematopoietic stem/progenitor cells, HSPCs) was used to prepare RNA. Total RNA was extracted using acid guanidine-thiocyanate-phenol–chloroform method and treated with DNase I (Qiagen, Germantown, MD, USA). RNA quality was assessed using 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA), and RNA integrity numbers were greater than 8 for all samples. cDNA libraries were constructed using NEBNext kit (New England Biolabs, Ipswich, MA, USA). Sequencing was performed on Illumina HiSeq4000 or NovaSeq (Illumina, San Diego, CA, USA).
The raw data have been deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database under accession number PRJNA679200.

Koralkova P., Belickova M., Kundrat D., Dostalova Merkerova M., Krejcik Z., Szikszai K., Kaisrlikova M., Vesela J., Vyhlidalova P., Stetka J., Hlavackova A., Suttnar J., Flodr P., Stritesky J., Jonasova A., Cermak J, & Divoky V. (2021). Low Plasma Citrate Levels and Specific Transcriptional Signatures Associated with Quiescence of CD34+ Progenitors Predict Azacitidine Therapy Failure in MDS/AML Patients. Cancers, 13(9), 2161.

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