Xponent version 3

Manufactured by DiaSorin

XPONENT, version 3.1 is a software application developed by DiaSorin for laboratory equipment. The core function of this software is to provide a platform for data analysis and reporting capabilities for diagnostic testing procedures.

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Lab products found in correlation

Luminex 200 platform, by DiaSorin (1 mentions) Power wave microplate reader, by Agilent Technologies (1 mentions) Luminex 200 system, by DiaSorin (1 mentions) Enzyme linked immunosorbent assay, by R&D Systems (1 mentions) Milliplex map kit, by Merck Group (1 mentions)

2 protocols using xponent version 3

Quantification of Mouse Chemokines

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Circulating mouse chemokines were quantified in serum/plasma using the MAP Mouse Cytokine/Chemokine Magnetic Bead Panel per the manufacturer's instructions (EMD Millipore, Billerica, MA). Chemokines tested included eotaxin, fractalkine, interferon‐gamma‐inducible protein 10, keratinocyte chemoattractant, monocyte chemotactic protein (MCP)‐1 (CCL2), MCP‐5, monokine induced by gamma interferon (MIG), macrophage‐derived chemokine (MDC), macrophage inflammatory protein (MIP)‐1α (CCL3), MIP1β (CCL4), MIP‐2, MIP‐3α, MIP‐3β, and thymus and activation regulated chemokine. Data were acquired on the Luminex200 platform (Luminex, Austin, TX) and analyzed using XPONENT, version 3.1 software (Luminex).

Kruger A.J., Fuchs B.C., Masia R., Holmes J.A., Salloum S., Sojoodi M., Ferreira D.S., Rutledge S.M., Caravan P., Alatrakchi N., Vig P., Lefebvre E, & Chung R.T. (2018). Prolonged cenicriviroc therapy reduces hepatic fibrosis despite steatohepatitis in a diet‐induced mouse model of nonalcoholic steatohepatitis. Hepatology Communications, 2(5), 529-545.

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Multiplex Cytokine and CRP Analysis

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Serum collected at day 18 of pregnancy was analysed for the presence of IFNγ, IL-1β, IL-6, IL-10, TNF-α together with mouse homologues of IL-8 (KC, LIX, MIP-2) using a multiplex assay according to the manufacturer’s protocol (Milliplex MAP kit, Merck Millipore, Billerica, MA, USA). All standards and samples were assayed in duplicate using a Luminex 200™ System (Luminex Corporation, Austin, TX, USA). Concentrations were determined from standard curves and analysed using xPONENT version 3.1 software (Luminex Corporation).
Mouse C-Reactive Protein was measured in duplicate using a commercially available Enzyme Linked Immunosorbent Assay (R&D Systems, Minneapolis, MN. USA) and optical density (450nm) read on a Powerwave microplate reader (BioTek Instruments, Winooski, VT, USA). Standard curves were generated using KC4 software (BioTek Instruments) and used to determine the concentration of CRP in each sample.

Stockham S., Stamford J.E., Roberts C.T., Fitzsimmons T.R., Marchant C., Bartold P.M, & Zilm P.S. (2015). Abnormal Pregnancy Outcomes in Mice Using an Induced Periodontitis Model and the Haematogenous Migration of Fusobacterium nucleatum Sub-Species to the Murine Placenta. PLoS ONE, 10(3), e0120050.

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