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Axio optical microscope

Manufactured by Zeiss
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Sourced in Germany

The AXIO optical microscope is a versatile instrument designed for high-quality imaging and analysis. It features advanced optics and illumination systems to provide clear, detailed observations of a wide range of samples. The core function of the AXIO is to enable users to examine and study specimens with precision and accuracy.

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Lab products found in correlation

Alpha300r confocal raman system, by WITec (1 mentions) Sirion 200, by Thermo Fisher Scientific (1 mentions) Blocking reagent, by Agilent Technologies (1 mentions) Envision system hrp labeled polymer anti rabbit, by Agilent Technologies (1 mentions) Cleaved caspase 3 asp175 antibody 9661, by Cell Signaling Technology (1 mentions) Antibody diluent solution, by Agilent Technologies (1 mentions) Mayer s hematoxylin, by Agilent Technologies (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Sa 5004, by Vector Laboratories (1 mentions) Aec reagent, by Agilent Technologies (1 mentions) Em ace600 carbon evaporator, by Leica (1 mentions)

Spelling variants of this product

Optical microscope (194 citations) Axio microscope (122 citations) Axio Imager M2 optical microscope (12 citations) Axio Observer Z1 optical microscope (6 citations) Axio Imager Z2 optical microscope (3 citations) AXIO-Vert.A1 optical microscope (3 citations) Axio.Scope optical microscope (2 citations) Axio Scope.A1 optical light microscope (2 citations) AXIO polarized optical microscope (2 citations) Axio Imager Z1 optical microscope (2 citations)

6 protocols using axio optical microscope

1

Characterization of Vanadate Photoconductor

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All bright-field and dark-field optical micrographs were captured using a ZEISS Axio optical microscope (ZEISS, Shanghai, China). The morphological details of the samples were characterized by an NT-MDT Prima AFM system (Apeldoorn, Netherlands) operating in semi-contact mode and a Hitachi SEM system with operation parameters of 10 kV and 10 μA. Raman and photoluminescence spectra were measured through a WITec Alpha300R confocal Raman system (WITec, Ulm, Germany) equipped with a 100X objective lens (NA: 0.95) and excited by a 532 nm laser. To prevent photodegradation during measurement, we used a laser power of 100 μW and limited the integration time to 1 s. For electrical transport and photoresponse measurements, the VP devices were tested inside a Cindbest probe-station system (Cindbest, Shenzhen, China) with a vacuum sample chamber (10−4 Pa). A Keithley 2450 source meter was used to apply the electrical voltage and examine the current signals, while a Zolix TLS3-X300P-G xenon lamp (Zolix, Beijing, China) served as the illumination source. To determine the elemental concentration of bulk VP crystals in different states, we performed XPS analysis using a Thermo ESCALAB 250Xi X-ray photo-electron spectroscopy system (Thermo Fisher, Waltham, MA, USA); the peak of adventitious carbon (284.8 eV) was used as the calibration reference for accurate elemental quantification.
Zhang X., Lv B., Wei H., Yan X., Peng G, & Qin S. (2024). Photodegradation and van der Waals Passivation of Violet Phosphorus. Nanomaterials, 14(5), 422.
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2

Microstructure and Fractography of Ti-6Al-4V

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To investigate the microstructure, a portion of each specimen was obtained from close to the center of the gauge section. The portions were cut along the longitudinal planes, and the metallographic samples were prepared using a standard mechanical grinding and polishing procedure. The Ti-6Al-4V metallographic specimens were then etched with Kroll’s reagent (2% HF, 6% HNO3, and 92% H2O). Optical microscopy examination of the microstructure was conducted using a Zeiss Axio optical microscope (Carl Zeiss, Oberkochen, Baden-Württemberg, Germany). Fractography images were conducted using a field-emission scanning electron Microscope (SEM) (Sirion 200, FEI, Hillsboro, OH, USA).
Xi J., Hu Y., Xing H., Han Y., Zhang H., Jiang J, & Nikbin K. (2021). The Low-Cycle Fatigue Behavior, Failure Mechanism and Prediction of SLM Ti-6Al-4V Alloy with Different Heat Treatment Methods. Materials, 14(21), 6276.
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3

Micromotor Delivery in Fasted Mice

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Mice underwent a fasting treatment for ~8 hours before the retention investigation. 0.1 mL suspension containing the enteric polymer-coated micromotors (~106 mL−1 in water) was orally administered into the mice. During the experiment, water feeding was maintained. As the controls, wax-coated passive Mg particles and wax-coated passive Mg/Au particles were prepared by incubating 0.05 g Mg particles or Mg/Au particles with 1 g paraffin wax at 75 °C overnight and then sequentially washed with chloroform, acetone, and pure water (47 (link)). Then, the control sample was orally administered. After 12 hours, both groups of mice were sacrificed, and the intestines were collected. Retention of micromotors was observed utilizing a Zeiss AXIO optical microscope at 5× magnification. The retained micromotors and control particles were counted using ImageJ (Fig. 5E). Dissolution of Mg after administration was characterized by optical imaging before and after acid treatment (fig. S12).
Wu Z., Li L., Yang Y., Hu P., Li Y., Yang S.Y., Wang L.V, & Gao W. (2019). A microrobotic system guided by photoacoustic computed tomography for targeted navigation in intestines in vivo. Science robotics, 4(32), eaax0613.
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4

Microstructure Analysis of 316L and 2507 Stainless Steel

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Stainless steel sheet specimens of grades 316L and 2507 were metallographically investigated to study their microstructure, the effects of the cutting procedures and the type of corrosion attack after exposure to the acidic solution. The alloy sheets were first cut and mounted in resin using a cold mounting procedure. The specimens were then ground with a range of grinding papers (P320, P600, P1200, P2500, and P4000), and polished with a 1 µm diamond suspension. After grinding and polishing, the specimens were etched with Beraha II color etchant and rinsed with laboratory grade alcohol. A Zeiss Axio optical microscope was used to examine the specimens.
Hren M., Kosec T., Lindgren M., Huttunen-Saarivirta E, & Legat A. (2021). Sensor Development for Corrosion Monitoring of Stainless Steels in H2SO4 Solutions. Sensors (Basel, Switzerland), 21(4), 1449.
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5

Immunohistochemical Analysis of Ki67 and Cleaved Caspase-3

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Tumors were harvested and fixed in formalin. Four-micrometer-thick sections were prepared from paraffin-embedded sections and rehydrated. Antigen retrieval was performed using Sodium Citrate Buffer (10 mmol/L Sodium Citrate, 0.05% Tween 20, pH 6.0) and Citric Acid Buffer (10 mmol/L Citric Acid, 0.05% Tween 20, pH 6.0) following routine protocols. Nonspecific signal was blocked using Blocking reagent (DakoCytomation). Slides were incubated overnight at 4°C with anti-Ki67 (SP6; ab16667 Abcam) or Cleaved Caspase-3 (Asp175) antibody #9661 (Cell Signaling Technology) antibodies diluted following the manufacturer's recommendation in Antibody diluent solution (DakoCytomation). Endogenous peroxidase activity was quenched using H2O2, and ffter several washes in PBS, EnVision+ System HRP-labeled polymer anti-rabbit were added as per requested by the manufacturer's (DakoCytomation), followed by HRP streptavidin (dilution 1:500, SA-5004, Vector). Slides were quickly washed twice in PBS and incubated in AEC+ reagent and counterstained with Mayer's hematoxylin (DakoCytomation). After washing in PBS, slides were mounted with Vectashield (Vector). Immunostaining was recorded with an AXIO optical microscope (Zeiss) equipped with a color AXIOCAM camera 105 (Zeiss, Oberkochen, Germany) and quantified using ImageJ. 15 fields per tumor (n = 2 per condition) were analyzed.
Lumeau A., Bery N., Francès A., Gayral M., Labrousse G., Ribeyre C., Lopez C., Nevot A., El Kaoutari A., Hanoun N., Sarot E., Perrier M., Pont F., Cerapio J.P., Fournié J.J., Lopez F., Madrid-Mencia M., Pancaldi V., Pillaire M.J., Bergoglio V., Torrisani J., Dusetti N., Hoffmann J.S., Buscail L., Lutzmann M, & Cordelier P. (2024). Cytidine Deaminase Resolves Replicative Stress and Protects Pancreatic Cancer from DNA-Targeting Drugs. Cancer Research, 84(7), 1013-1028.
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6

Imaging Mg-based Micromotors and MCs

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SEM images of the Mg-based micromotors were acquired with a field emission scanning electron microscope (FEI Sirion) at an operating voltage of 10 keV (Fig. 2A). The samples were coated with a 5-nm carbon layer to improve the conductivity (Leica EM ACE600 Carbon Evaporator). The bright field and fluorescence microscopic images of the micromotors and the MCs were taken with a Zeiss AXIO optical microscope (Fig. 2B, figs. S3 and S4). To observe the structure of the DOX-loaded micromotors and MCs using fluorescence imaging, the micromotors and the MCs were stained with FITC-albumin. Labeling of FITC-albumin onto the micromotors was carried out by dip-coating the micromotors-loaded glass slides in a 0.2 mL of FITC-albumin solution (0.2 mg mL−1), followed by dip-coating in an alginate solution (2%, w/v). Labeling of the FITC-albumin onto the MCs was conducted by adding FITC-albumin into the gelation solution.
Wu Z., Li L., Yang Y., Hu P., Li Y., Yang S.Y., Wang L.V, & Gao W. (2019). A microrobotic system guided by photoacoustic computed tomography for targeted navigation in intestines in vivo. Science robotics, 4(32), eaax0613.
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