Lrrk2 in 1

DMEM-F12 (Dulbecco's modified Eagle's medium), penicillin–streptomycin, gentamicin and fetal bovine serum (FBS) were purchased from Gibco-Invitrogen (Carlsbad, CA, USA). GSK 2578215A and LRRK2-IN-1 were purchased from Tocris Bioscience (Minneapolis, MN, USA). Stealth RNAi duplexes were purchased from Life Technologies (Carlsbad, CA, USA). BCA protein assay was from Pierce (Rockford, IL, USA). The pDsRed2-mito vector was provided by Clontech (BD Biosciences, San Jose, CA, USA), GFP-LC3 was provided by Dr. JM Fuentes (Universidad de Extremadura, Badajoz, Spain), Drp1-GFP was provided by T Wilson and Dr. S Strack (Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA, USA) and mRFP-GFP-LC3 was provided by Dr. E Kneck (Laboratory of Cellular Biology, Centro de Investigación Príncipe Felipe, Valencia, Spain). Anti-4 hydroxynonenal and LRRK2 antibodies were purchased from Abcam (Cambridge, UK); anti-p62 from BD (San Jose, CA, USA) and Alexa Fluor 488 were from Molecular Probes (Carlsbad, CA, USA), Invitrogen (Carlsbad, CA, USA); and anti-acetylated tubulin was from Sigma-Aldrich (St. Louis, MO, USA). The TUNEL method (MEBSTAIN Apoptosis Kit) was purchased from MBL (Carlsbad, CA, USA). Mdivi-1 was purchased from Sigma-Aldrich.

Trichostatin A, tubacin and nocodazole were from Sigma Aldrich, PTL from Eurodiagnostico, LRRK2-IN1, TAE684 and CZC25146 from the Michael J. Fox Foundation, GSK2578215A from Tocris, compound 68 (ID 9108605) and compound 70 (ID 9119202) from Chembridge Corporation (San Diego, USA), and GNE-0877 and GNE-7915 from MedchemExpress (USA), and MLi2 from MRC PPU, Dundee, UK. Natural streptolysin-O was from Abcam (ab63978), and non-hydrolyzable GTP test kit from Jena Bioscience (NK-102).