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B6c3f1 j mice

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B6C3F1/J mice are a widely used mouse strain that is a hybrid of the C57BL/6J and C3H/HeJ mouse strains. They are commonly used in biomedical research, including studies related to cancer, toxicology, and immunology.

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Lab products found in correlation

Hsd athymic nude foxn1nu, by Inotiv (1 mentions) Balb c mice, by Jackson ImmunoResearch (1 mentions) B6eic3sn a a ts 1716 65dn ts65dn, by Jackson ImmunoResearch (1 mentions) Potassium edta, by BD (1 mentions)

8 protocols using b6c3f1 j mice

1

Generation of Lgals3-/R200S Mutant Mice

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B6C3F1/J mice were obtained from Jackson Labs (Bar Harbor, ME, USA) and were used to generate our B6;C3‐Lgals3tm1Vari mutant model, which will be referred to as Lgals3‐R200S.
Once this line was generated, we backcrossed it for two generations with purebred C57BL/6J mice. All animal procedures followed the protocol (PIL‐16‐01‐002) approved by the Institutional Animal Care and Use Committee of the Van Andel Institute. Mice were housed in climate‐controlled conditions (25 °C, 55% humidity, and 12 h of light alternating with 12 h of darkness) and fed a standard LabDiet Rodent Chow 5010 (Purina Mills, Gray Summit, MO, USA). Animals for our studies were euthanized at embryonic day 13 to collect mouse embryonic fibroblasts (MEFs) or at a 36‐week time point for bone studies.
Maupin K.A., Diegel C.R., Stevens P.D., Dick D, & Williams B.O. (2022). Mutation of the galectin‐3 glycan‐binding domain ( Lgals3‐R200S) enhances cortical bone expansion in male mice and trabecular bone mass in female mice. FEBS Open Bio, 12(10), 1717-1728.
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2

Intracranial Glioma Models and Toca 511/5-FC Therapy

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All animal protocols and experiments were approved by the UCLA or Explora institutional
animal care and use committees. Six- to 8-week-old female nude mice (Hsd:Athymic
Nude-Foxn1nu; Envigo) or B6C3F1/J mice (Jackson Laboratory) were bred
and maintained under specific pathogen-free conditions. To establish intracranial gliomas,
U-87 (1 × 105) or Tu-2449 (1 × 104) cells were stereotactically
injected into the right frontal lobe in mice, as described previously.8 (link) In U-87 tumor models, Toca 511 (low dose:
3.4 × 104 transducing units [TU] or high dose: 2 × 106 TU) was given
by stereotactic injection on day 5, and daily intraperitoneal (i.p.) administration of
5-FC (500 mg/kg once daily or twice daily) or phosphate buffered saline (PBS) was started
on day 18 for 7 consecutive days. This 7-day cycle was repeated at intervals of every 1–2
weeks. In Tu-2449 tumor model studies conducted at UCLA, Toca 511 (2 × 106 TU)
was given by stereotactic injection on day 4, and daily i.p. administration of 5-FC or PBS
was started on day 10 for 7 consecutive days. This cycle was repeated at intervals of
every 10 days. In independent Tu-2449 studies conducted at Tocagen, Toca 511 was
administered as above at 3 × 106 TU followed by 4 cycles of 5-FC administered
twice a day for 4 days every 2 weeks, or at a lower dose (1.6 × 104 TU), and
continuous 5-FC administered once a day for 14 or 21 consecutive days.
Hiraoka K., Inagaki A., Kato Y., Huang T.T., Mitchell L.A., Kamijima S., Takahashi M., Matsumoto H., Hacke K., Kruse C.A., Ostertag D., Robbins J.M., Gruber H.E., Jolly D.J, & Kasahara N. (2017). Retroviral replicating vector–mediated gene therapy achieves long-term control of tumor recurrence and leads to durable anticancer immunity. Neuro-Oncology, 19(7), 918-929.
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3

Topical DBP Exposure in Mice

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To mimic the bioassay employed in our previous carcinogenicity and DNA adduct studies [5 (link), 25 (link), 27 (link), 28 (link)], eight week-old female B6C3F1/J mice (Jackson Laboratories, Bar Harbor, ME) were used in this study. Mice were quarantined for about 1 week before treatment. All mice were kept on a 12-hr light:12-hr dark cycle, maintained at 50% relative humidity and 21± 2°C, and were fed with AIN-93M diet (5% corn oil), and water ad libitum. The bioassay was carried out in accordance with the NIH Guide for the Care and Use of Laboratory Animals and was approved by Institutional Animal Care and Use Committee.
Mice received topical treatment of DBP (24 nmol, 3 times per week for 5 weeks) in the oral cavity and were euthanized 48 h after the last dose; this time point was selected based on our previous study showing maximum levels of DNA adducts [25 (link)]. Animals treated with dimethyl sulfoxide (DMSO) as the vehicle were used as control. At termination, mice were euthanized by CO2 asphyxiation; oral tissues were isolated from the same anatomic sites of mice treated with vehicle or DBP (soft tissues of the oral cavity, including the buccal mucosa and floor of the mouth as well as soft tissues attached to the hard palate) and pooled together for DNA extraction.
Sun Y.W., Chen K.M., Imamura Kawasawa Y., Salzberg A.C., Cooper T.K., Caruso C., Aliaga C., Zhu J., Gowda K., Amin S, & El-Bayoumy K. (2017). Hypomethylated Fgf3 is a potential biomarker for early detection of oral cancer in mice treated with the tobacco carcinogen dibenzo[def,p]chrysene. PLoS ONE, 12(10), e0186873.
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4

Radiolabeled Disulfiram for Cancer Imaging

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All of the chemicals were purchased from commercial vendors and used without further purification. Disulfiram was purchased from USP (Cat. No. 1224008, Rockville, MD), and dissolved in a solution of 15% DMSO and 85% Cremophor EL (10 mg/ml). F-DPA and [18F]-F-DPA were synthesized in Dr. Steven H. Liang laboratory (Massachusetts General Hospital). Balb/c mice and B6C3F1/J mice were purchased from Jackson Laboratory. All animal experiments were approved by the Institutional Animal Use and Care Committee (IACUC) at Massachusetts General Hospital, and carried out in accordance with the approved guidelines.
Yang J., Yang J., Wang L., Moore A., Liang S.H, & Ran C. (2017). Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2. Scientific Reports, 7, 8298.
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5

Ts65Dn Mouse Model Genotyping Protocol

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Ts65Dn mouse colony female B6EiC3Sn a/A-Ts(1716)65Dn (Ts65Dn) and male B6C3F1/J mice were purchased from the Jackson Laboratory (Bar Harbor, ME). The mouse colony was housed and bred in the Animal Facilities of the Barcelona Biomedical Research Park (PRBB, Barcelona, Spain, EU). All animal procedures met the guidelines of the European Community Directive 86/609/EEC and were approved by the Local Ethics Committee. The mice were housed under a 12:12 h light–dark schedule (lights on at 8:00 a.m.) in controlled environmental conditions of humidity (60%) and temperature (22 ± 2°C) with food and water ad libitum. Both Ts65Dn and euploid mice were genotyped by qPCR, following the Jackson laboratories protocol (https://www.jax.org/research-and-faculty/tools/cytogenetic-and-down-syndrome-models-resource/protocols/cytogenic-qpcr-protocol).
Gómez de Salazar M., Grau C., Ciruela F, & Altafaj X. (2018). Phosphoproteomic Alterations of Ionotropic Glutamate Receptors in the Hippocampus of the Ts65Dn Mouse Model of Down Syndrome. Frontiers in Molecular Neuroscience, 11, 226.
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6

Intraperitoneal ABP Dosing in Mice

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The guidelines established by the National Institutes of Health Office of Laboratory Animal Welfare were adhered to for the use of animals with housing conditions. All protocols were reviewed and approved by the University of Minnesota Institutional Animal Care and Use Committee. Eleven weeks old adult male B6C3F1/J mice (Jackson Laboratory, Bar Harbor, ME) were used in this study. The animals (N = 5) were given intraperitoneal injection of ABP at a dose of 40 mg/kg body weight (100 μL of 80% DMSO : 20% H2O). The blood was collected from the abdominal aorta into vacutainers containing potassium EDTA (Becton Dickinson), 24 h following dosing. The Hb was processed as described.35 (link)
Pathak K.V., Chiu T.L., Amin E.A, & Turesky R.J. (2016). Methemoglobin Formation and Characterization Hemoglobin Adducts of Carcinogenic Aromatic Amines and Heterocyclic Aromatic Amines. Chemical research in toxicology, 29(3), 255-269.
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7

Preclinical Study of N171-82Q HD Mice

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Male N171–82Q HD mice were mated to female B6C3F1/J mice (Jackson Laboratory, ME) to generate the experimental mice. Male mice were used in our current study, because we found gender-dependent phenotypic differences in N171–82Q HD mice, therefore we use male N171–82Q mice for this preclinical study as we did previously (Jiang et al., 2013 (link); Jiang et al., 2012 ). Mice were randomly divided into six groups. DNP or placebo (1% sodium bicarbonate, pH 7.0) was orally given to the mice by gavage from 8 weeks old to the end of the study. Animals were housed under specific pathogen-free conditions with a reversed 12-h light/dark cycle maintained at 23 °C and provided with food and water ad libitum. All animal experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by Johns Hopkins University Animal Care and Use Committee.
Wu B., Jiang M., Peng Q., Li G., Hou Z., Milne G.L., Mori S., Alonso R., Geisler J.G, & Duan W. (2017). 2,4 DNP improves motor function, preserves medium spiny neuronal identity, and reduces oxidative stress in a mouse model of Huntington’s disease. Experimental neurology, 293, 83-90.
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8

Striatal Injection of α-Synuclein Fibrils

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All housing and procedures were performed according to the National Institutes of Health Guide for the Care and Use of Experimental Animals and approved by the University of Pennsylvania Institutional Animal Care and Use Committee. The injection studies described used 2–3-month-old female B6C3F1/J mice (stock no. 100010; The Jackson Laboratories). Timed-pregnant CD1 mice for neuronal cultures were obtained from Charles River Laboratories. Animals were maintained on a 12 h light/dark schedule and provided with food ad libitum.
Sonicated PFFs (5 μg total in 2.5 μl of PBS) were stereotaxically injected in the dorsal striatum using the following coordinates: AP +0.2 mm, M/L 2.0 mm and depth beneath skull 2.6 mm. Each mouse received a single unilateral injection of the indicated PFFs. At the post-injection time points indicated, mice were transcardially perfused with heparinized PBS, and brains were removed for fixation in 70% ethanol in 150 mM NaCl, pH 7.4.
Balana A.T., Mahul-Mellier A.L., Nguyen B.A., Horvath M., Javed A., Hard E.R., Jasiqi Y., Singh P., Afrin S., Pedretti R., Singh V., Lee V.M., Luk K.C., Saelices L., Lashuel H.A, & Pratt M.R. (2024). O-GlcNAc forces an α-synuclein amyloid strain with notably diminished seeding and pathology. Nature Chemical Biology, 20(5), 646-655.
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