Mice were treated with systemic thermal therapy (STT; core temperature of 39.5 ± 0.5°C, 6 h) and allowed to return to baseline temperature prior to homing studies as described14 (link),27 (link). LPS (1 mg/kg; Sigma) was injected i.p. 6 h prior to trafficking studies14 (link). Neutralizing polyclonal anti-CXCL9 or anti-CXCL10 Ab30 (link) (100 μg/mouse) or polyclonal isotype IgG control (cat# BE0091 100 μg/ mouse; BioXCell) were injected i.p. 3 h prior to competitive homing studies. Murine activated CD8+ T cells were pretreated with pertussis toxin (100 ng/ml, 2.5 h; Sigma), or anti-mouse CXCR3 blocking Ab (cat# 126526 100 μg/mL, 1h; Biolegend). Human activated T cells were pretreated with pertussis toxin, human CXCR3 functional blocking Ab (cat# LS-C6283-500 40 μg/ml, 30 min; LifeSpan BioSciences, Inc39 (link)), or human recombinant CXCL10 (500nM, 30 m; Peprotech) to desensitize CXCR340 (link).
Recombinant human cxcl10
Manufactured by Thermo Fisher Scientific
Sourced in United States
Recombinant human CXCL10 is a chemokine protein produced using recombinant DNA technology. It is involved in the recruitment and activation of immune cells.
Automatically generated - may contain errors
Lab products found in correlation
Pertussis toxin, by Merck Group (2 mentions)
Polyclonal isotype igg control, by BioXCell (2 mentions)
Anti mouse cxcr3 blocking ab, by BioLegend (2 mentions)
Lipopolysaccharides (lps), by Merck Group (2 mentions)
Protamine, by Merck Group (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Human serum albumin (hsa), by Grifols (1 mentions)
Mk 2206 2hci, by Selleck Chemicals (1 mentions)
Recombinant human tnf α, by Sino Biological (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
Bay11 7082, by Selleck Chemicals (1 mentions)
Sb203580, by Selleck Chemicals (1 mentions)
6 protocols using recombinant human cxcl10
1
Investigating Immune Cell Trafficking Regulation
2
Investigating Immune Cell Trafficking Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were treated with systemic thermal therapy (STT; core temperature of 39.5 ± 0.5°C, 6 h) and allowed to return to baseline temperature prior to homing studies as described14 (link),27 (link). LPS (1 mg/kg; Sigma) was injected i.p. 6 h prior to trafficking studies14 (link). Neutralizing polyclonal anti-CXCL9 or anti-CXCL10 Ab30 (link) (100 μg/mouse) or polyclonal isotype IgG control (cat# BE0091 100 μg/ mouse; BioXCell) were injected i.p. 3 h prior to competitive homing studies. Murine activated CD8+ T cells were pretreated with pertussis toxin (100 ng/ml, 2.5 h; Sigma), or anti-mouse CXCR3 blocking Ab (cat# 126526 100 μg/mL, 1h; Biolegend). Human activated T cells were pretreated with pertussis toxin, human CXCR3 functional blocking Ab (cat# LS-C6283-500 40 μg/ml, 30 min; LifeSpan BioSciences, Inc39 (link)), or human recombinant CXCL10 (500nM, 30 m; Peprotech) to desensitize CXCR340 (link).
3
CXCL10 effects on E. coli growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
E. coli (≈3 × 105 cells per sample well) was treated with recombinant human CXCL10 (Peprotech, Rocky Hill, NJ, USA) in sterile water stabilized with 0.3% human serum albumin (HSA; Grifols Therapeutics Inc., Research Triangle Park, NC, USA) or an equal volume of 0.3% HSA alone as vehicle. Protamine (Sigma-Aldrich, St. Louis, MO, USA) was similarly prepared. Assay conditions were as previously described (13 (link)) with modifications. Bacteria were inoculated into 10 ml LB broth and incubated overnight at 37°C in a shaking incubator. The bacterial cultures were diluted into LB broth prewarmed to 37°C to an OD of 0.1 to 0.3 at 600 nm; subcultures were then grown under the same conditions to an OD of ≈0.6 at 600 nm. These mid-log-phase bacteria were diluted to the assay concentration of ≈3 × 106 cells/ml in 10 mM potassium phosphate buffer (pH 7.4) supplemented with 1% Trypticase soy broth (13 (link)). After 2 h of incubation, serial dilutions were plated on LB and incubated overnight at 37°C to determine CFU per milliliter.
+ Expand
4
CXCL10 Stimulation of Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HO-8910 and A2780 cell lines as well as human lymphatic tube endothelial cells (HLECs) were obtained from Dr. Feng at Southeast University. HO-8910 and A2780 cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), while HLECs were cultured in Endothelial Cell Medium (ScienCell Research Laboratories, Inc.) with 10% FBS. The cultures were maintained at 37°C in a humidified incubator with 5% CO2. To stimulate CXCL10 in vitro, we purchased recombinant human CXCL10 from Peprotech and incubated cells with 100 and 200 ng/ml hCXCL10 for 48 h.
5
Reconstitution of Chemokine CXCL10 and Peptide CTTC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant human CXCL10 was purchased from PeproTech (Rocky Hill, NJ). CXCL10 was dissolved in sterile H2O containing 0.3% (wt/vol) human serum albumin (HSA; Grifols, Los Angeles, CA) at a concentration of 1 mg ml−1 and stored at −80°C in 10-µl aliquots to limit freeze-thaw cycles to no more than one. The CTTC peptide was commercially synthesized and capped by amidation on the C terminus by United Biosystems (Herndon, VA) (see Text S2B in the supplemental material). Lyophilized CTTC was dissolved in sterile H2O at a concentration of 5 mg ml−1 and stored in 20-µl aliquots as described above. Sequence and structure verification was carried out as described in Text S2B .
6
Cytokine Signaling in Colon Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CC cell lines (SW480/SW620) were purchased from the American Type Culture Collection (ATCC, USA), cultivated within L15 media that contained 10% FBS (VivaCell, Shanghai, China) and incubated in the ventilated environment under 37 °C and 95% air and 5% CO2 conditions. Recombinant human TNF-α was obtained from Sino Biological, and recombinant human CXCL10 was purchased from Peprotech. At the final concentrations listed, the following basic pharmacological inhibitors were used: SB203580 (the inhibitor of p38 MAPK, Selleck), LY294002 (the inhibitor of PI3K, Selleck), MK-2206 2HCI (the inhibitor of Akt, Selleck), BAY11-7082 (the inhibitor of NF-κB, Selleck) and CHIR-99021 (the inhibitor of GSK-3β, MCE). All the cellular studies were carried out under specified and standard conditions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!