Glucose oxidase peroxidase god pod assay kit

For nanoparticle synthesis, tetraethylorthosilicate (TEOS), urea, cetylpyridinium bromide (CPB), cyclohexane, isopropanol, acetone, 37% hydrochloric acid solution, citric acid, and trisodium citrate were used. All chemicals were purchased from Sigma-Aldrich (Milan, Italy). For enzyme immobilization and activity measurements, β-glucosidase from almond (molecular weight 135,000 Da for the dimer), sodium hydroxide, TEPI (95% volume ratio), and glucose oxidase-peroxidase (GOD-POD) assay kit from Sigma-Aldrich (Milan, Italy) were used. The activity of BG, that is, the amount of enzyme that liberates 1 μmol of glucose per minute at pH 5.0 and 37 °C (salicin as substrate) was provided from the producer (≥6 U/mg).
Citrate buffer solution at pH 5.0 was prepared as specified in [35 ]. A total of 3.9 mg of citric acid was dissolved in 205 mL of bidistilled water, and 8.7 mg of trisodium citrate was dissolved in 295 mL of bidistilled water. The two solutions were then mixed and the volume was brought to 1 L.

Tetraethylorthosilicate (TEOS), urea, cetyltrimethylammonium bromide (CTAB), cyclohexane, pentanol, acetone, 37% hydrochloric acid solution, citric acid, and trisodium citrate were used to synthesize WSNs with pentanol (WSN-p). WSNs with isopropanol (WSN-ipa) were prepared in the same way as WSN-p, but using isopropanol instead of pentanol. All chemicals were purchased from Sigma-Aldrich (Milan, Italy). For enzyme physical adsorption and kinetic measurements, cellulase from Tricoderma Reesei and glucose oxidase-peroxidase (GOD-POD) assay kit from Sigma-Aldrich (Milan, Italy) were used. Citrate buffer solution at pH 5.0 was prepared by dissolving 3.9 mg of citric acid in 205 mL of bidistilled water, and 8.7 mg of trisodium citrate in 295 mL of bidistilled water. The two solutions were mixed and the volume was brought to 1 L by diluting with bidistilled water.

Tetraethyl orthosilicate
(TEOS), urea, cetyltrimethylammonium bromide (CTAB), cyclohexane,
anhydrous 2-propanol (ACS reagent), ethanol, hydrochloric acid solution
(37.0% wt. in water), β-glucosidase from almonds (molecular
weight 135 kDa for the dimer, product number 49290, specific activity
≥4 U/mg, measured as micromoles of glucose liberated per minute
at pH 5 and 37 °C with salicin as substrate), citric acid, trisodium
citrate dihydrate, sulfuric acid (95.0–98.0% wt.), glucose
oxidase–peroxidase (GOD-POD) assay kit, and potassium bromide
were purchased from Sigma-Aldrich (Milan, Italy).