Tumour tissues were fixed with 4% paraformaldehyde and cut into 4‐μm‐thick sections. Immunohistochemistry (IHC) was performed according to the protocol. The sections were incubated with rabbit anti‐FOXM1 (1:100, 20,459, CST), anti‐PDK1 (1:100, 18,262‐1‐AP; Proteintech), anti‐GLUT1 (1:200, 66,290‐1‐Ig; Proteintech), anti‐ LDHA (1:200, 66,287‐1‐Ig; Proteintech) and anti‐HK2 (1:100, 22,029‐1‐AP; Proteintech) antibodies overnight at 4°C. The sections were then sequentially incubated with a biotinylated secondary antibody (PV‐9000; ZSGB‐Bio) to detect the primary antibodies. Images were obtained using TissueFAXS systems (TissueGnostics).
Anti pdk1
Manufactured by Proteintech
Sourced in China
Anti-PDK1 is a primary antibody that recognizes the PDK1 (Pyruvate Dehydrogenase Kinase 1) protein. PDK1 is a serine/threonine protein kinase that plays a key role in regulating the activity of the pyruvate dehydrogenase complex, which is involved in glucose metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Anti ldha, by Proteintech (1 mentions)
Anti hk2, by Proteintech (1 mentions)
Pv 9000, by ZSGB-BIO (1 mentions)
Anti glut1, by Proteintech (1 mentions)
Anti c myc, by Cell Signaling Technology (1 mentions)
Anti hif 1α, by Santa Cruz Biotechnology (1 mentions)
Anti idh2, by Cell Signaling Technology (1 mentions)
Anti idh1, by Proteintech (1 mentions)
Anti pfkl, by Proteintech (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti glut1, by Cell Signaling Technology (1 mentions)
Octyl αkg, by Cayman Chemical (1 mentions)
Anti ldha, by Cell Signaling Technology (1 mentions)
Anti mfn1, by Cell Signaling Technology (1 mentions)
Dextran sulfate sodium (dss), by Merck Group (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti gfp, by Merck Group (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti akt, by Proteintech (1 mentions)
3 protocols using anti pdk1
1
Immunohistochemical Analysis of Tumor Markers
2
Antibodies and Reagents for Cell Signaling
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The following antibodies used in this study were purchased from the indicated sources: anti-HIF-1α and anti-GAPDH (Santa Cruz); rabbit IgG, anti-GFP, and anti-Flag (Sigma); anti-β-actin, anti-GLUT1, anti-c-Myc, anti-IDH2, anti-H2AFZ, anti-MFN1, and anti-LDHA (Cell Signaling Technology); and anti-IDH1, anti-PFKL, and anti-PDK1 (proteintech). Octyl-α-KG was obtained from Cayman Chemical. DSS was obtained from Sigma. The primers and oligo DNA used in this study are listed in Table S1 .
3
Caco-2 and MA104 Cell Line Based Assays
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Caco-2 cell line was from cell bank of Wuhan University (Wuhan, China). The MA104 cell line was from the cell bank of Sun Yat-Sen University (Guangzhou, China). The RV Wa strain (G1P[8]) was was from the immunology institute of the Third Military Medical University (Chongqing, China). The glucose assay kit was obtained from Shanghai Rong Sheng Biotechnology Co., LTD (Shanghai, China). The glucose 6phosphatase and phosphoenolpyruvate carboxylase assay kits were from Solarbio (Beijing, China). Anti-JNK, anti-AKT, anti-SIK2 and anti-PDK1 were obtained from Proteintech (Wuhan, China). Anti-beta-actin, anti-rabbit IgG were obtained from Cell Signaling Technology (Boston, USA).
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