The concentrations of cytokines in the lung were measured by analyzing BAL on days 0, 3, 7, 11 and 21 days post-infection. The levels of interleukin (IL)-2, IL-6, interferon (IFN)-γ , and tumor necrosis factor (TNF)-α , RANTES, Eotaxin, and MIG in the lysate were measured using a Bio-Plex multiplex bead array kit (Bio-Rad, Hercules, CA). The Bio-Plex assay was performed in accordance with the manufacturer’s instructions.
Bio plex multiplex bead array kit
Manufactured by Bio-Rad
The Bio-Plex multiplex bead array kit is a lab equipment product that enables the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded magnetic beads coated with specific capture antibodies to measure multiple targets in a single well.
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3 protocols using bio plex multiplex bead array kit
1
Multiplex Cytokine Analysis in BAL
2
Cytokine Profiling in Murine Lung Tissue
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The concentrations of cytokines in lung tissue were measured. On days 2 and 4 post-infection, 8 mice per group were sacrificed and whole lung tissue was collected and immediately frozen. Lungs were thawed, weighed and lysed using the Bio-Plex Cell Lysis Kit (Bio-Rad, Hercules, CA). The levels of interleukin (IL)-6, IL-10, IL-12(p70), interferon (IFN)-γ, and tumor necrosis factor (TNF)-α in the lysate were measured using a Bio-Plex multiplex bead array kit (Bio-Rad, Hercules, CA). The Bio-Plex assay was performed in accordance with the manufacturer’s instructions.
3
Cytokine Profiling in AAV9 Vector Model
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The concentrations of 23 cytokines in plasma and CSF were measured on days 0, 14 and 56 days post-inoculation of the AAV9 vectors using a Bio-Plex multiplex bead array kit (Bio-Rad, Hercules, CA). The Bio-Plex assay was performed in accordance with the manufacturer’s instructions.
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