Bbr chloride

BBR chloride (pharmaceutical primary standard) and trisodium citrate (Na₃C₆H₅O₇, purity > 99%) were purchased from Sigma Aldrich. Glycerol was supplied by Fisher Scientific (purity > 99%). Nutrient broth and nutrient agar were provided by Titan Biotech, India. Bi-distilled water through a Milli-Q® system was used.
Two bulk silver bars (99.999% purity) and two bulk zinc bars with dimensions (L × W × T) of 150 × 10 × 0.5 mm were used as electrodes for the electrochemical process.
Two bacterial strains, namely, MRSA (Gram-positive bacteria) and S. enteritidis (Gram-negative bacteria) were provided by the Department of Bacteriology, National Institute of Hygiene and Epidemiology, Vietnam.

All animal experimental procedures were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals and were approved by the IEC for Clinical Research and Animal Trials of the First Affiliated Hospital of Sun Yat-sen University (approval number: [2021]048). C57BL/6 J mice (8 weeks, male) were provided from animal research center of the first affiliated hospital of Sun Yat-sen University (Guangzhou, China) and maintained at 22–24°C, 50% relative humidity, with a 12 h light/dark period. C57BL/6 J mice were fed with a standard chow diet and randomly assigned to six groups (n = 5 for each group). The mini osmotic pump (MODEL 2004, ALZET, United States) was subcutaneously implanted into mice to deliver either saline or Ang II (0.8 mg/kg/day) for 4 weeks. The 1% choline chloride (Sigma, United States) was administered via drinking water for 4 weeks. BBR chloride (Sigma, United States) was given at a dosage of 150 mg/kg/day for 4 weeks by intragastric administration.

BBR chloride was purchased from Sigma-Aldrich (pharmaceutical primary standard). Glycerol was provided by Fisher Scientific (purity > 99%). Nutrient broth and nutrient agar were obtained from Titan Biotech, India. Bi-distilled water from a Milli-Q system (18.2 MΩ·cm at 25 °C) was used. All other chemicals were of analytical grade and used without further purification.
Two bacterial strains isolated from clinical samples, namely, methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli O157:H7 (E. coli O157:H7) were provided by the National Institute of Hygiene and Epidemiology, Vietnam.