Astx cas no 472 61 7

ASTX (CAS No. 472-61-7) and STZ (CAS No. 18883-66-4) were purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA), while fetal bovine serum (FBS) was obtained from HyClone Laboratories, Inc. (Logan, UT, USA). Antibodies specific to Nrf2 (BS1258) and receptor activator of nuclear factor (NF)-κB ligand (RANKL; ALX-804-243) were purchased from Bioworld Technology, Inc. (St. Louis Park, MN, USA) and Enzo Life Sciences, Inc. (Farmingdale, NY, USA), respectively. Antibodies specific to interleukin-1β (IL-1β; sc-52012), interferon-γ (IFN-γ; sc-57208), tumor necrosis factor α (TNF-α; sc-52746), and β-actin (sc-47778) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). 2′,7′-Dichlorodihydrofluorescein-diacetate (DCF-DA), and anti-osterix (ab209484), anti-osteopontin (OPN; ab8448), anti-heme oxygenase 1 (HO-1; ab13248), anti-bone morphogenetic protein 2 (BMP2; ab214821), and anti-γ-H2AX (ab26350) antibodies were purchased from Abcam (Cambridge, UK). Unless otherwise specified, other reagents were purchased from Sigma-Aldrich Co. LLC, while laboratory consumables were from Falcon Labware (Becton-Dickinson, Franklin Lakes, NJ, USA).

ASTX (CAS No. 472-61-7) and STZ (CAS No. 18883-66-4) were obtained from Sigma-Aldrich Co. LLC (St. Louis, MI, USA). ML385 (CAS No. 846557-71-9/Nrf2-specific inhibitor) and fetal bovine serum (FBS) were obtained from Tocris Biosciences (Avonmouth, Bristol, UK) and HyClone Laboratories, Inc. (Logan, UT, USA), respectively. Antibodies specific to cyclooxygenase-2 (COX-2; BS1076), Nrf2 (BS1258), and runt-related transcription factor 2 (RUNX2, BS2831) were purchased from Bioworld Technology, Inc. (St. Louis Park, MN, USA). Receptor activator of nuclear factor (NF)-κB ligand (RANKL; ALX-804-243) was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA), while 2′,7′-dichlorodihydrofluorescein-diacetate (DCFH-DA), osterix (ab209484), osteopontin (ab8448), and γ-H2AX (ab26350) were from Abcam (Cambridge, UK). The 8-hydroxy-2′-deoxyguanosine (8-OHdG; sc-66036) and antibodies specific to cathepsin K (CTSK; sc-48353), interleukin (IL)-1β (sc-52012), and β-actin (sc-47778) were provided from Santa Cruz Biotechnology (Santa Cruz, CA, USA). AGE was offered from BioVision (Milpitas, CA, USA). Unless otherwise specified, chemicals were purchased from Sigma-Aldrich Co. LLC, while laboratory consumables were from Falcon Labware (Becton-Dickinson, Franklin Lakes, NJ, USA).