Mixotrophically grown transformants in 96-well microtiter plates were irradiated with low light (LL; white fluorescent light), UV light (fluorescent light with UV, generated using a T8 ReptiSun 10.0 UVB fluorescent bulb (Zoo Med Laboratories, CA)), or a monochromatic LED (blue, 450 nm; green, 530 nm; and red, 660 nm) at 30 μmol/m2/s. After 4 hours of light treatment, the transformants were mixed with luciferin (potassium salt; Promega, Madison, WI) at a final concentration of 100 μM and transferred into white 96-well microtiter plates (PerkinElmer, Norwalk, CT). Bioluminescence from the transformants was measured with a highly sensitive bioluminescent detector (model CL96; Churitsu Electric Corp., Japan).
White 96 well microtiter plates
Manufactured by PerkinElmer
White 96-well microtiter plates are a type of laboratory equipment used for various assays and experiments. These plates have 96 individual wells that can hold small volumes of samples or reagents. The plates are white in color, which can be beneficial for certain applications that require optical measurements or detection.
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Lab products found in correlation
2 protocols using white 96 well microtiter plates
1
Bioluminescence Assay of Transformants
2
Estrogenic and Antiestrogenic Activities Assessment
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The estrogenic and antiestrogenic activities of the extracted lipPOP mixture (fraction F1) and PFAA mixture (fraction F3-W2) were assessed using stable transfected MVLN cells (provided by M Pons, INSERM, Montpellier, France). Experiments were carried out at cell passage 2-10. See Bonefeld-Jorgensen et al. (2005) (link) for detailed information on the ER assay. In short, MVLN cells were seeded in white 96-well microtiter plates (Perkin Elmer) with a density of approximately 8.5 × 10 4 cells/well. The serum mixtures were tested alone (XER) and for competitive measurements (XERcomp) upon co-exposure with 24 pM E2 (Sigma-Aldrich). The dry serum mixtures were reconstituted as previously described on the day of analysis (Bonefeld-Jorgensen et al. 2006) (link). The cells were harvested after exposure to the serum mixtures for 20 h, and luciferase activity and protein content were measured. An E2 concentrationresponse control (1.5-300 pM) was performed in parallel in each assay.
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