Spinach ferredoxin nadp reductase

Manufactured by Merck Group

Spinach ferredoxin-NADP+ reductase is an enzyme that catalyzes the transfer of electrons from ferredoxin to NADP+, a key step in the light-dependent reactions of photosynthesis.

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Lab products found in correlation

Spinach ferredoxin, by Merck Group (3 mentions) Biliverdin ixα, by Frontier Scientific (2 mentions) Glucose 6 phosphate dehydrogenase, by Merck Group (2 mentions) Bovine liver catalase, by Merck Group (2 mentions) Factor va, by Prolytix (1 mentions) Sodium ascorbate, by Merck Group (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by GoldBio (1 mentions) Reduced nadph, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Glucose 6 phosphate (g6p), by Merck Group (1 mentions) Cholesterol, by Merck Group (1 mentions) 7α hydroxycholesterol, by Avanti Polar Lipids (1 mentions)

3 protocols using spinach ferredoxin nadp reductase

Biliverdin IXα Extraction and Enzymatic Assays

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Biliverdin IXα was purchased from Frontier Scientific Inc., Logan, UT, spinach ferredoxin-NADP⁺ reductase, spinach ferredoxin, NADP+, glucose-6-phosphate, and glucose-6-phosphate dehydrogenase were purchased from Sigma-Aldrich. Hemin was obtained from Fluka. Manduca sexta insecticyanin was a gift of Dr. J. Winzerling, University of Arizona. Biliverdin IXγ was obtained by extracting the insecticyanin sample with methanol and removing the precipitated protein by centrifugation. Human factor Xa, prothrombin and thrombin were obtained from Calbiochem. Factor Va was obtained from Haematologic Technologies, and S-2239 was obtained from Diapharma.

Veiga A.B., Ribeiro J.M., Francischetti I.M., Xu X., Guimarães J.A, & Andersen J.F. (2014). Examination of the Ligand-Binding and Enzymatic Properties of a Bilin-Binding Protein from the Poisonous Caterpillar Lonomia obliqua. PLoS ONE, 9(6), e95424.

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Biliverdin Synthesis and Purification

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Biliverdin IXα was purchased from Frontier Scientific Inc. (Logan, UT). DMSO, reduced NADPH, spinach ferredoxin, spinach ferredoxin NADP⁺ reductase, heme (hemin chloride), sodium ascorbate, and bovine liver catalase were purchased from Sigma (St. Louis, MO). IPTG was purchased from Gold Biotechnology Inc. (St. Louis, MO).

Sigala P.A., Morante K., Tsumoto K., Caaveiro J.M, & Goldberg D.E. (2016). In-Cell Enzymology to Probe His-Heme Ligation in Heme Oxygenase Catalysis,. Biochemistry, 55(34), 4836-4849.

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Cholesterol Metabolite Synthesis

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1,4-Cholestadiene-3-one
was obtained from
Research Plus (Barnegat, NJ), and 7α-hydroxy cholesterol and
7α-hydroxy-4-cholesten-3-one were from Avanti Polar Lipids (Alabaster,
AL). All other chemicals, including cholesterol, spinach ferredoxin,
spinach ferredoxin-NADP⁺-reductase, bovine liver catalase,
glucose 6-phosphate, glucose-6-phosphate dehydrogenase, and methyl-β-cyclodextrin,
were purchased from Sigma-Aldrich (St. Louis, MO).

Frank D.J., Waddling C.A., La M, & Ortiz de Montellano P.R. (2015). Cytochrome P450 125A4, the Third Cholesterol C-26 Hydroxylase from Mycobacterium smegmatis. Biochemistry, 54(46), 6909-6916.

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