Cfi plan apo lambda 100x oil objective

Phase-contrast microscopic analysis was performed for E. coli BL21(DE3) strains with CatIB formation. 1 µL of each sample was transferred to a microscope slide and covered with a coverslip. The microscope slide was positioned upside down on the desk of an inverted Nikon Eclipse Ti2 microscope (Nikon GmbH, Düsseldorf, Germany). The sample was observed with a CFI Plan Apo Lambda 100X Oil objective (Nikon GmbH, Düsseldorf, Germany) and images were taken with a Thorlab camera DCC154M-GL (Thorlabs Inc., Newton, NJ, US). To determine the percentage of CatIB-producing cells during the cultivation process, cells with visible inclusion bodies at the poles were manually counted (n_cells = 45–200).

Phase-contrast microscopic analysis was performed for E. coli BL21(DE3) strains with CatIB formation and for control strains. Cell suspension samples from cultivation experiments were taken before CatIB purification and analyzed by microscopy. A volume of 1 µL was applied on a microscope slide and covered with a coverslip. The microscope slide was positioned upside down on the desk of an inverted Nikon Eclipse Ti2 microscope (Nikon GmbH, Düsseldorf, Germany). The sample was observed with a CFI Plan Apo Lambda 100X Oil objective (Nikon GmbH, Düsseldorf, Germany) and images were taken with a Thorlab camera DCC154M-GL (Thorlabs Inc., Newton, New Jersey, USA). Analysis of cell images were performed with Fiji ImageJ [44 (link)] to determine the areas of at least 100 inclusion bodies and cells.

Phase-contrast microscopic analysis was performed for E. coli BL21(DE3) strains with CatIB formation strains. 1 µL of each sample was given on a microscope slide and covered with a coverslip. The microscope slide was positioned upside down on the desk of an inverted Nikon Eclipse Ti2 microscope (Nikon GmbH, Düsseldorf, Germany). The sample was observed with a CFI Plan Apo Lambda 100X Oil objective (Nikon GmbH, Düsseldorf, Germany) and images were taken with a Thorlab camera DCC154M-GL (Thorlabs Inc., Newton, New Jersey, USA). The amount of cells that carried CatIBs was counted by analyzing at least 50 cells per CatIB variant.