Axio cam 305 camera

Fixed specimens from the authors’ collection were rinsed in water, stained with acetocarmine, dehydrated, cleared in xylene and mounted in Canada balsam. Parasites were examined for morphological features including body size, position and size of the vaginae, genital spine number, shape of haptor and suckers and shape and size of hamuli, when present. Morphological features were photographed using a Nikon AZ100M microscope (Nikon, Netherlands) fitted with a 0.5X, 1X and 4X objectives as well as a Nikon U3 digital camera. Measurements were captured using the Nikon NIS software. Small structures were examined, measured and photographed using a Zeiss Imager Axio10 compound microscope (Zeiss, Germany) fitted with a Zeiss Axio cam 305 camera (Zeiss, Germany) and Zeiss Zen Blue elements (Zeiss, Germany) software program. Finally, type drawings for all recorded chelonian polystome species (see Morrison and Du Preez, 2011 ) were also investigated for their morphology, genital spine number and intervaginal distances.

The capacity of SA4503 and PRE084 to mitigate oxidative stress was compared with that of (+)-PTZ. The 661W cells were seeded on coverslips and exposed to tBHP (55 µM) for 2 hours in the presence or absence of SA4503 (3, 50, and 100 µM), PRE084 (3, 50, and 100 µM) or (+)-PTZ (3 and 50 µM). Control cells received no tBHP nor treatment with Sig1R ligands. To detect intracellular reactive oxygen species (ROS), cells were incubated with 5 µM CellROX Green Reagent (ThermoFisher). CellROX Green Reagent is a cell-permeant dye that fluoresces weakly in a reduced state but brightly upon oxidation by ROS with an absorption and emission maxima of approximately 485 and 520 nm, respectively. It is used in living cells to detect hydroxyl, peroxyl, peroxynitrite, and hydroxyl radicals. DAPI was used to detect cell nuclei. Immunofluorescent detection was performed using a Zeiss Axio Imager D2 microscope (Carl Zeiss, Göttingen, Germany) and Zeiss Axiocam 305 camera equipped with ZenPro software. Fluorescence intensity was quantified using National Institutes of Health Image J 1.48v software (National Institutes of Health, Bethesda, MD). For this analysis, three independent experiments were performed with three repetitions per assay.