Propylene glycol monocaprylate (Capryol 90) and caprylocaproyl macrogol-8-glyceride (Labrasol) (Gattefosse, Gennevilliers, France) were gift samples from Colorcon Asia (Mumbai, India), while propylene glycol monocaprylic ester (Sefsol 218) and Kolliphor RH-40 were a gift sample from Nikko Chemicals (Tokyo, Japan) and BASF, Mumbai, respectively. Diethylenemonoglycol ether (Carbitol), Polyethylene Glycol (PEG-200, 400, 600), Isopropyl myristate (IPM), glycerol triacetate (Triacetin), methanol (HPLC-Grade), and Distilled Water were purchased from E-Merck (Mumbai, India). Polyoxyethylene sorbitan monolaurate (Tween-20), polyoxy ethylene sorbitan monostearate (Tween-60), polyoxyethylene sorbitan monooleate (Tween-80), ethanol, isopropyl alcohol were procured from S.D. Fine Chemicals (Mumbai, India). Fluorescent dye 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and 4',6'-diamidino-2 phenylindole (DAPI) were purchased from Sigma Aldrich, USA. Eagle's minimal essential medium (MEM), fetal bovine serum (FBS), MTT (3 (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye and antibiotic solutions were purchased from Himedia, India. Rutin (97 %) bioactives were purchased from Sigma-Aldrich Chemical Company (St. Louis, Missouri). All the reagents used for study were of analytical grade.
Antibiotic solution
Manufactured by HiMedia
Sourced in India, United States
Antibiotic solution is a sterile, liquid-based product designed for laboratory use. It is a mixture of one or more antibiotics, formulated to inhibit the growth of bacteria in controlled experimental settings. The core function of this product is to provide a standardized antibiotic source for researchers and scientists working in microbiological fields.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by HiMedia (5 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Anti mouse cy5, by Jackson ImmunoResearch (2 mentions)
Mouse monoclonal anti α tubulin, by Merck Group (2 mentions)
Mouse monoclonal anti gcp3, by Santa Cruz Biotechnology (2 mentions)
Anti gcp6, by Santa Cruz Biotechnology (2 mentions)
Mouse monoclonal anti actin, by BD (2 mentions)
Rnase a, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by HiMedia (2 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by HiMedia (2 mentions)
Rpmi 1640 media, by HiMedia (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by HiMedia (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Trizol rna isolation reagent, by Thermo Fisher Scientific (1 mentions)
Precision plus protein dual color standard, by Bio-Rad (1 mentions)
Ecl substrate, by Santa Cruz Biotechnology (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
17 protocols using antibiotic solution
1
Formulation and Characterization of Rutin Loaded Self-Emulsifying Drug Delivery System
2
Cervical Cancer Cell Lines for HPV Research
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Human cervical cancer cell lines with known HPV positivity for HPV type 16 – SiHa, HPV type 18 – HeLa; and HPV-negative C33a were originally procured from ATCC. The materials used in the study have been listed along with their source of procurement. The materials used in the study have been listed along with their source of procurement. DMEM (#AL111-18X500ML), MEM (#AT154), antibiotic solutions (#A018), bovine serum albumin fraction V (#RM10409) were procured from HiMedia Laboratories Pvt. Ltd. (Mumbai, India), Pierce™ BCA Protein Assay Kit (#23225), exosome-depleted serum One Shot™ format (#A2720803), Precision Plus Protein Dual Color Standards from Bio-Rad, USA (#161-0374), High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™; #4368814), TRIzol RNA Isolation Reagent (#AM9738) were procured from Thermo Fischer Scientific (Waltham, USA). ExoEnrich™ (#PEC-50), ExoLyseP™ (#PEL-25P) were purchased from ExoCan Healthcare Technologies Ltd. (Pune, India). ECL-substrate (#SC-2048) from Santa Cruz Biotechnology, Inc. (Dallas, USA). All the antibodies were procured from Santacruz Biotechnology, Inc. (USA) and Sigma (St. Louis, USA) (Supplementary Table 1 ) and oligos used in the study were procured from Eurofins scientific (Supplementary Table 2 ). Millipore PVDF membrane (#HVLP04700), RNAse A (#P4170), and all other reagents unless specified were procured from Sigma.
3
Immunostaining Analysis of Centrosomal Proteins
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DAPI was purchased from Sigma. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic solutions were purchased from HiMedia, Inc. (Mumbai, India). Mouse monoclonal anti-TACC3, mouse polyclonal anti-GCP4, rabbit polyclonal anti-GCP6, and anti-γ-tubulin were obtained from Abcam; mouse monoclonal anti-α-tubulin and mouse monoclonal anti-γ-tubulin were purchased from Sigma; mouse monoclonal anti-GCP3, anti-GCP6 and goat polyclonal pericentrin antibody were obtained from Santa Cruz Biotechnology, Inc.; mouse monoclonal anti-actin was purchased from BD Biosciences; and rabbit polyclonal phospho-TACC3 antibody was purchased from Cell Signalling, USA. Rabbit polyclonal chTOG antibody was obtained from Abcam. The secondary antibodies, anti-mouse FITC, anti-rabbit TRITC, anti-mouse Cy5, anti-goat and anti-rabbit Cy5 were obtained from Jackson ImmunoResearch.
4
Immunofluorescence and Western Blot Analysis of Tubulin-Associated Proteins
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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic solutions were purchased from HiMedia, Inc. (Mumbai, India). Lipofectamine RNAiMAX, Lipofectamine 3000, and pre-stained protein ladder, 10 to 250 kDa were purchased from Thermo Fisher Scientific, U.S.A. PEI MAX- transfection agent from Polysciences. Mouse monoclonal anti-α-tubulin was purchased from Sigma (Cat # T5168); Mouse monoclonal anti-γ-tubulin for WB & IF (Cat # sc-17787), mouse monoclonal anti-TACC3 (Cat # sc-376883), mouse polyclonal anti-GCP4 (Cat # sc-271876), mouse monoclonal anti-GCP3 (Cat # sc-373758), anti-GCP6 (Cat # sc-374063), and mouse monoclonal anti-GFP antibody (Cat # sc-9996) were obtained from Santa Cruz Biotechnology, Inc.; rabbit polyclonal anti-ch-TOG antibody was obtained from Abcam (Cat # ab-236981); mouse monoclonal anti-actin was purchased from BD Biosciences (Cat # 612656); and rabbit monoclonal phospho-TACC3 antibody was purchased from Cell Signalling, U.S.A. (Cat # 8842). The secondary antibodies, anti-rabbit Alexa 568 and anti-mouse Cy5 were obtained from Jackson ImmunoResearch. DAPI was purchased from Sigma. GFP-Trap agarose beads used for IP were from Chromotech. Agarose A/G beads used for IP were from Santa Cruz Biotechnology, Inc.
5
Immunomodulatory Effects of Emodin
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ConA (Concanavalin A), propidium iodide (PI), and MTT [3-(4, 5-di-methylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] were each purchased from Sigma (St. Louis, MO). RPMI 1640, fetal bovine serum (FBS), and antibiotic solution were obtained from Himedia India (Mumbai, India). Murine T H 1/T H 2/T H 17 cytometric beads array (CBA) kits were bought from BD Biosciences (San Jose, CA). RNase A, DMSO, and Emodin (cat# 324694; purity by HPLC: 96.1%) were purchased from Merck (Darmstadt, Germany). All other chemicals used were of analytical grade from local firms.
6
Glucose Uptake Assay in Cell Lines
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The chemicals and reagents used in this study are as follows: Dulbecco’s Modified Eagle’s medium (DMEM) high glucose (#AL219A, HiMedia, India), DMEM without glucose (#AL186, HiMedia), adjustable multichannel pipettes and a pipettor (BenchTop Lab Systems, Saint Louis, Missouri, USA), fetal bovine serum (FBS) (#RM10432, HiMedia), Dulbecco’s phosphate-buffered saline (D-PBS) (#TL1006, HiMedia), antibiotic solution (#A018, HiMedia), 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) (#13195, Invitrogen, USA), metformin (#PHR1084, Sigma-Aldrich, USA), acarbose (#A8980, Sigma-Aldrich), anti-mouse GLUT4–fluorescein isothiocyanate (FITC) antibody (#NBPI-49533F, Novus Biologicals, USA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (#4060, HiMedia), and dimethyl sulfoxide (DMSO) (#PHR1309, Sigma-Aldrich).
7
Comparative Cytotoxicity in A549 and V79 Cells
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A549 adenocarcinoma human alveolar basal epithelial cells and V79 Chinese hamster lung fibroblasts were purchased from the National Centre for Cell Science (NCCS), Pune. Dulbecco’s Modified Eagle’s Medium (DMEM) and antibiotic solution were purchased from HiMedia, Mumbai, India. Fetal bovine serum (FBS) was purchased from Gibco, Waltham, MA, USA. Other chemicals such as MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, acridine orange, ethidium bromide, and cholesterol were purchased from HiMedia, India. The 100 bp DNA ladder and DNA gel-loading dye (6x) were purchased from Thermo Fisher Scientific, Mumbai, India. Dimethyl sulfoxide (DMSO), chloroform, isopropanol, and ethanol were purchased from SRL chemicals, Mumbai, India.
8
Gefitinib-Eudragit RL100 Nanoparticle Formulation
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Gefitinib and Eudragit® RL100 were obtained as gift samples from Neon Laboratories Ltd. (Mumbai, India) and Dr Reddy’s Laboratories Ltd. (Hyderabad, India), respectively. Polyvinyl alcohol (PVA) with molecular weight 13,000 was obtained from National Chemicals (Gujarat, India). Polyvinylpyrrolidone (PVP) K30 with molecular weight 40,000 was procured from HiMedia® Laboratories Pvt. Ltd. (Mumbai, India). Methanol high-performance liquid chromatography (HPLC) grade and dimethyl sulfoxide (DMSO) were purchased from Finar Ltd. (Ahmedabad, India). Highly pure water (18.2 MΩ cm resistivity, Milli Q) was produced in the laboratory. Dulbecco’s Modified Eagle’s Media (DMEM) and trypsin ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Fetal bovine serum (FBS), sterile phosphate-buffered saline (PBS), 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT), and antibiotic solution (a mixture of penicillin, streptomycin, and clarithromycin) were obtained from HiMedia® Laboratories Pvt. Ltd. All other chemicals were purchased from Merck Ltd. (Mumbai, India).
9
Metallohydrogel Synthesis and Characterization
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2,4-Di-tert-butylphenol was purchased from Sigma-Aldrich. Manganese acetate, aspartic acid and indomethacin were obtained from alfa SRL. Gemcitabine was bought from BLD pharm. Commercially available forms of formaldehyde, ethanol, and methanol was employed without additional purification. Milli Q water was used for the formation of metallohydrogel. Other common solvents of the analytical reagent grade were utilised. DMEM medium, sodium bicarbonate, antibiotic solution and FBS, propidium iodide and calcein AM were purchased from Himedia, Sigma-Aldrich, and invitrogen.
10
Differentiation and DENV Infection of MEG-01 Cells
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MEG-01 cells, a human megakaryoblastic leukemia cell line, were grown in RPMI medium (Lonza) with 10% FBS and antibiotic solution (Himedia Laboratories) with 5% CO2. For further differentiation, MEG-01 cells were treated with growth factor TPO (100 ng/mL; PeproTech) for 24 h prior to experiments. For DENV infection, MEG-01 cells were incubated with DENV for 3 h at 37°C, washed with PBS, and resuspended in RPMI-1640 medium with 10% FBS/1X antibiotics and kept at 37°C with 5% CO2 in a cell culture incubator for different experiments. To inhibit different cellular mechanisms, AKT, PI3K, PKCα, and mTOR inhibitors (AKT inhibitor IV (5.0 µM), Ly294002 (10.0 µM), and HBBDE (10.0 µM) from Sigma Aldrich; Torin1 from Cell Signaling Technology) were used respectively as per-requisite of experiments after infection.
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