Polyvinyl alcohol (pva)

Manufactured by Thermo Fisher Scientific

Sourced in United States, Belgium, United Kingdom, Germany, China, Italy

Polyvinyl alcohol (PVA) is a synthetic polymer commonly used in a variety of laboratory applications. It is a water-soluble, odorless, nontoxic, and biodegradable material. PVA is known for its excellent film-forming, emulsifying, and adhesive properties, making it a versatile choice for various laboratory equipment and procedures.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions) Polyvinyl alcohol (pva), by Merck Group (5 mentions) Chitosan, by Merck Group (4 mentions) Ethanol, by Thermo Fisher Scientific (4 mentions) Sodium chloride (nacl), by Merck Group (4 mentions) Polycaprolactone, by Merck Group (4 mentions) Methanol, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Methanol, by Merck Group (3 mentions) Acetic acid, by Chempur (3 mentions) Methylene chloride, by Chempur (3 mentions) Trichloroacetic acid, by Chempur (3 mentions) Sulforhodamine, by Thermo Fisher Scientific (3 mentions) Tris buffer, by Chempur (3 mentions) Cacl2, by Carlo Erba (3 mentions) Sodium alginate, by Merck Group (3 mentions) Dichloromethane dcm, by Thermo Fisher Scientific (3 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Irinotecan, by Selleck Chemicals (2 mentions) Hyaluronic acid, by Lifecore Biomedical (2 mentions)

102 protocols using polyvinyl alcohol (pva)

Mesophase Pitch-based Conductive Inks

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4.1 NCS slurry precursor preparation 8 0.1 g of mesophase pitch (MP, Momentum Materials Solutions (https://momentummaterials.ca/mesophase-pitch), softening point: 250 1C, ash o150 ppm, coking value Z90%, toluene insolubles: 65 AE 3% and quinoline insolubles: 42 AE 3%), 0.2 g n-butanol and 5.0 g 10% polyvinyl alcohol (PVA, Alfa Aesar, 86-89% hydrolyzed, low molecular weight)/water were ballmilled together, producing a uniform MP/PVA ink. A colloidal silica suspension containing 0.5 g silica (Ludox SM-30, Ludox AS-40, NexSil-8040, or NexSil-125-40 with an average particle size of 8, 22, 50 and 85 nm, respectively) was added to 1.0 g of 1,3-propanediol (PD) : water (mass ratio 1 : 1) to produce a silica suspension. The silica suspensions were added to the MP/PVA ink and then ball-milled to obtain the MP/PVA/PD/silica ink (or slurry), which was then degassed for 15 min to remove any bubbles.

Atwa M., Li X., Wang Z., Dull S., Xu S., Tong X., Tang R., Nishihara H., Prinz F, & Birss V. (2021). Scalable nanoporous carbon films allow line-of-sight 3D atomic layer deposition of Pt: towards a new generation catalyst layer for PEM fuel cells. Materials horizons, 8(9).

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Octreotide-loaded PLGA Nanoparticles

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The following reagents, drugs, and chemicals were used in this study: PVA (Mw 20,000–30,000, ACROS ORGANICS); Octreotide acetate (Shanghai Tash Biotechnology Co., Ltd); PLGA (L/G 50/50, Mw 35,000, Birmingham, Germany). Polyvinyl alcohol (PVA, Mw 30,000–70,000, Acros, NJ); All other chemicals (Sinopharm chemical reagent Co., Ltd, Shanghai, China); Sandostatin (Novartis, Ltd.); ALT, AST, NO, MDA, SOD, NOS, GSH and Hyp assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China); ET,TXB₂ and TNFα (Liberation and Exemption Institute of Science and Technology Development Center of PLA General Hospita, China).

Han B., Tang H., Liang Q., Zhu M., Xie Y., Chen J., Li Q., Jia J., Li Y., Ren Z., Cong D., Yu X., Sui D, & Pei J. (2021). Preparation of long-acting microspheres loaded with octreotide for the treatment of portal hypertensive. Drug Delivery, 28(1), 719-732.

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Fabrication of PVA-based Hydrogels

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A 20% (m/v) solution of PVA (MW 60,000 from Sigma Aldrich) was made by dissolving PVA in distilled water at 100 °C and 500 rpm. Meanwhile, a 5% (m/v) gelatin solution was made in PBS. A 1 mg/mL chitosan solution was made in PBS with 0.5% (v/v) glacial acetic acid. The three solutions were autoclaved at 120 °C for 15 minutes and then cooled to 37 °C. An equal amount of PVA was poured into each well of a 48-well plate under aseptic conditions. Next, 100 μL of the 1% (m/v) gelatin, 1 mg/mL chitosan, or 0.1% (v/v) PEG 8000 (Thermo Fisher) was added to the designated wells to create a PVA-gelatin, PVA-chitosan, or PVA-PEG hydrogels, respectively. The PVA-based hydrogels were stirred with a pipette tip to mix. Next, the baculovirus stock solution was added to each well and stirred a final time. PBS was added to hydrogels to serve as a mock. The hydrogels were frozen overnight and thawed for two hours to constitute one freeze-thaw cycle. The freeze-thaw cycle was repeated one to four times depending on the hydrogel.

Schaly S., Islam P., Boyajian J.L., Thareja R., Abosalha A., Arora K., Shum-Tim D, & Prakash S. (2023). Controlled and customizable baculovirus NOS3 gene delivery using PVA-based hydrogel systems. PLOS ONE, 18(9), e0290902.

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Polymeric Nanocarriers for Drug Delivery

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PLGA (lactide:glycolide: 75:25, M_w: 4,000–15,000) and Pluronic F127 (PF127) were purchased from Sigma (St. Louis, MO, USA). Hyaluronic acid (HA, M_w: 66–90 kDa) was purchased from Lifecore Biomedical (Chaska, MN, USA). Polyvinyl Alcohol (PVA, M_w: 100 kDa) was purchased from Fisher Scientific (Pittsburgh, PA, USA). Chitosan oligosaccharide of pharmaceutical grade (M_w: 1.2 kDa, 95% deacetylation) was purchased from Zhejiang Golden Shell Biochemical Co. Ltd (Zhejiang, China). The WST-1 cell proliferation reagent was purchased from Roche Diagnostics (Mannheim, Germany). Fetal bovine serum (FBS) and penicillin/streptomycin were purchased from Invitrogen (Carlsbad, CA, USA). The F-12K and DMEM cell culture media were purchased from ATCC (Manassas, VA, USA). Doxorubicin was purchased from LC laboratories (Woburn, MA, USA). Irinotecan was purchased from Selleck Chemicals (Houston, TX, USA). All other chemicals were purchased from Sigma unless specifically mentioned otherwise.

Wang H., Agarwal P., Zhao S., Xu R.X., Yu J., Lu X, & He X. (2015). Hyaluronic acid-decorated dual responsive nanoparticles of Pluronic F127, PLGA, and chitosan for targeted co-delivery of doxorubicin and irinotecan to eliminate cancer stem-like cells. Biomaterials, 72, 74-89.

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Affinity Purification of Actin-Binding Proteins

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Phenyl-Sepharose CL-4B, CaCl₂ and EGTA were purchased from Sigma-Aldrich (St. Louis, MO). IAANS and phalloidin were purchased from Invitrogen (Carlsbad, CA). Affi-Gel 15 affinity media were purchased from Bio-Rad (Hercules, CA). Malachite Green Oxalate and poly(vinyl alcohol) were purchased from Fisher Scientific (Pittsburgh, PA)

Swindle N., Albury A.N., Baroud B., Burney M, & Tikunova S.B. (2014). Molecular and Functional Consequences of Mutations in the Central Helix of Cardiac Troponin C. Archives of biochemistry and biophysics, 548, 46-53.

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Formulation and Characterization of PEG-PLGA Nanoparticles

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Poly (ethylene glycol)-methyl ether-block -poly (lactide-co-glycolide) (PEG-PLGA_50:50, Mn_PEG 2000 Da and 10,000 Da Mn_PLGA), dimethyl sulfoxide (DMSO), and coumarin 6 dye were purchased from Sigma Aldrich (St. Louis, MO, USA). Rhodamine 6G dye, dichloromethane (DCM), polyvinyl alcohol (PVA) (87–89% hydrolyzed, high molecular weight), acetonitrile, phosphate buffer saline (PBS), polyvinyl chloride (PVC) tubing, syringes (glass and plastic), syringe needles, and polytetrafluoroethylenes (PTFE) based consumables such as syringe filters (0.45 μm), evaporating dish, and magnetic stirring bars were obtained from Fisher Scientific. All other chemicals and reagents used in this study were of analytical grade.

Sagoe P.N., Velázquez E.J., Espiritusanto Y.M., Gilbert A., Orado T., Wang Q, & Jain E. (2023). Fabrication of PEG-PLGA Microparticles with Tunable Sizes for Controlled Drug Release Application. Molecules, 28(18), 6679.

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Quantification of Citreoviridin in Rice

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Except where noted otherwise, deionized water (Nanopure II, Thermo Scientific, Waltham, MA, USA) was used in the preparation of all reagents. The primary analytical standard of CTV was produced by Hayashi Pure Chemical Ind., Ltd. (Osaka, Japan). For spiking of rice samples, a standard material containing both CTV and iso-CTV prepared at the USDA-NCAUR (Peoria, IL, USA) was used [21 (link)]. Acetonitrile and methanol were HPLC grade and were purchased from Fisher Scientific (Hampton, NH, USA), as was polyvinyl alcohol (PVA). 1-1′carbonyldiimidazole (CDI), 4,6-dimethyl-2-oxo-2H-pyran-5-carboxylic acid (IDHA), 4,6-dimethyl-α-pyrone (DMP), and threose were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were reagent grade or better and purchased from major suppliers.

Maragos C.M., Uchiyama Y., Kobayashi N., Kominato F, & Sugita-Konishi Y. (2019). Development and Characterization of Monoclonal Antibodies for the Mycotoxin Citreoviridin. Toxins, 11(11), 630.

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Chitosan-based Mucoadhesive Formulation

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Chitosan (MW 100,000–300,000), propranolol hydrochloride, agar powder, porcine stomach mucin, acetonitrile, methanol and orthophosphoric acid (HPLC grade) were purchased from Sigma-Aldrich (Germany). Potassium dihydrogen phosphate, sodium hydroxide, potassium thiocyanate, potassium chloride and calcium chloride were purchased from May & Baker (Dagenham, England). Poly vinyl alcohol (25% polymerization degree 5.00–2000), Carbopol 971P (CP971P), PEG 400, propylene glycol, were purchased from Fisher Chemical (Leicestershire UK). Metoprolol (99.87%); The internal standard was supplied by Sedico Company, Egypt.

Mohamad S.A., Salem H., Yassin H.A, & Mansour H.F. (2020). Bucco-Adhesive Film as a Pediatric Proper Dosage Form for Systemic Delivery of Propranolol Hydrochloride: In-vitro and in-vivo Evaluation. Drug Design, Development and Therapy, 14, 4277-4289.

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Synthesis of Metal Oxide Nanomaterials

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Ethanol, acetone, hydrazine
hydrated (N₂H₄·H₂O), polyvinylpyrrolidone
(PVP), sodium hydroxide (NaOH), polyvinyl alcohol (PVA), sodium nitrate
(NaNO₃), sulfuric acid (H₂SO₄), hydrochloric
acid (HCl), cobalt(II) chloride hexahydrate (CoCl₂·6H₂O), and nickel(II) chloride hexahydrate (NiCl₂·6H₂O) were purchased from Fisher Scientific. Potassium permanganate
(KMnO₄) and ethylene glycol were purchased from Merck,
India, and graphite powder (mean particle size of <20 mm) were
purchased from Sigma-Aldrich. All the chemicals were used without
further purification. Distilled water was used throughout the experiment.

Makkar P, & Ghosh N.N. (2020). Snowflake-Like Dendritic CoNi Alloy-rGO Nanocomposite as a Cathode Electrode Material for an All-Solid-State Flexible Asymmetric High-Performance Supercapacitor Device. ACS Omega, 5(18), 10572-10580.

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Synthesis and Characterization of Molecularly Imprinted Polymers

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Acetic acid, azobisisobutytonitrile (AIBN), chloroform, ethylene glycol dimethacrylate (EGDMA), methacrylic acid (MAA), methanol, high molecular weight (146–186 kDa) polyvinyl alcohol (PVA) and toluene were all purchased from Fisher Scientific UK (Loughborough, Leicester, UK). All were of analytical quality or high-performance liquid chromatography (HPLC) grade and used without purification. The templates andarine and RAD-140 were purchased from Biosynth Carbosynth (Compton, Berkshire, UK). Testosterone and estradiol were purchased from Merck (Gillingham, Dorset, UK).

Sullivan M.V., Fletcher C., Armitage R., Blackburn C, & Turner N.W. (2023). A rapid synthesis of molecularly imprinted polymer nanoparticles for the extraction of performance enhancing drugs (PIEDs). Nanoscale Advances, 5(19), 5352-5360.

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