Tr cn100 capillary column

Fatty acids were determined using an Agilent gas chromatograph (Model GC1000) equipped with a flame ionization detector (FID) and a TR‐CN100 capillary column (60 m length, 0.25 lm film, 0.2 mm internal diameter; Teknokroma) (Asadi et al., 2010). The column temperature was kept at 45°C for 4 min and increased up to 175°C with a rate of 13°C/min. Then reached to the final column temperature (215°C at 4°C/min rate) and remain constant for 60 min. Figure 1 shows the sample obtained pick for fatty acid analysis of GSO by gas chromatography.

Fatty acids were analyzed in feed and meat using the UNE-EN ISO 5508 method and by gas chromatography [20 ]. The fat was extracted using a cold decantation method [21 (link)] using Folch’s solution (chloroform:methanol) 2:1 and butylhydroxytoluene (BHT). For methylations, 0.2 N sodium methylate was added and stabilized by adding a 3% solution of sulfuric acid in anhydrous methanol. Finally, n-hexane was added and reheated to promote dissolution of the esters. Subsequently, the injection was carried out in the gas chromatograph (Thermo Finnogan Trace GC Ultran, Milan, Italy) using an autosampler (Thermo Scientific AS 3000, Milan, Italy) and a polar TR-CN100 capillary column (Teknokroma, Barcelona, Spain). For the separation of fatty acids, three temperature ramps were used, from 70 to 250 °C, and helium was used as a carrier gas (flow of 3.2 mL min⁻¹). The methyl esters were identified by the retention times of the reference standards (FAME mix 37 from Sigma Aldrich, Darmstadt, Germany) and were quantified using the methyl ester of undecanoic acid as an internal standard and with the calibration lines of each fatty acid. Atherogenic index and thrombogenic index were calculated following Chen and Liu [22 (link)]. All the determinations were done by duplicate.