The phosphotungstic negative stain method was used for morphology assessment of nanoliposomes (NNL, NNL-miR-1296). Simply, a drop of each sample was applied to separate copper girds coated with carbon film and left to dry in the air. Samples were treated with 2% phosphotungstic acid solution and further analyzed using JEM-1400 Plus TEM from JEOL (Tokyo, Japan).
Jem 1400 plus tem
Manufactured by JEOL
Sourced in Japan, United States, Germany
The JEM-1400 Plus TEM is a transmission electron microscope (TEM) developed by JEOL. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of research and industrial applications. The core function of the JEM-1400 Plus is to magnify and image samples at the nanoscale level, allowing users to study the structure and properties of materials in detail.
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Lab products found in correlation
Oneview camera, by Ametek (6 mentions)
Orius sc600 ccd camera, by Ametek (6 mentions)
Uc7 ultramicrotome, by Leica (5 mentions)
Oneview digital camera, by Ametek (4 mentions)
Ultracut ultramicrotome, by Leica (3 mentions)
Uct ultramicrotome, by Leica (3 mentions)
Epon resin, by Serva Electrophoresis (3 mentions)
Em uc7 ultramicrotome, by Leica (3 mentions)
Zetasizer nano zsp, by Malvern Panalytical (2 mentions)
Jem 1400plus, by JEOL (2 mentions)
Nanovan, by Nanoprobes (2 mentions)
Qev size exclusion column, by Izon Science (2 mentions)
Amicon ultrafiltration tube, by Merck Group (2 mentions)
Ultrostain, by Leica (2 mentions)
Ruby 8 mpx ccd camera, by JEOL (2 mentions)
Ruby ccd camera, by JEOL (2 mentions)
Jsm 7610f sem, by JEOL (1 mentions)
Oneview 4k camera, by Ametek (1 mentions)
300 mesh carbon coated copper grids, by Agar Scientific (1 mentions)
Jsm 7610f scanning electron microscope, by JEOL (1 mentions)
86 protocols using jem 1400 plus tem
1
Phosphotungstic Acid Staining of Nanoliposomes
2
Transmission Electron Microscopy of Extracellular Vesicles
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Samples were fixed with Karnovsky's fixative followed by staining with 2.0% of uranyl acetate. Briefly, 5 μl of MDVs was placed on Formvar/carbon‐coated copper 200 mesh grids (EMS) and mixed with 5 μl of uranyl acetate for 10–20 s. Excess stain was blotted off and grids were dried. Samples were examined with Jeol® JEM‐1400 Plus TEM (Jeol®, Tokyo, Japan), equipped with ORIUS SC600 CCD camera (Gatan®, Abingdon, United Kingdom), and Gatan Microscopy Suite program (DigitalMicrograph, Gatan®, UK).
3
Visualizing Nano-Sized Extracellular Vesicles
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NDMVs were fixed for 3 h in PBS with 2% glutaraldehyde and 2% paraformaldehyde (PFA) prior to analysis by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). For TEM, a copper grid was floated on top of 10 μL of an NDMV suspension for 10 min and washed with one drop of PBS and deionized water. NDMVs attached to the grid were floated on top of 10 μL 2% uranyl acetate and 3% lead citrate to stain for 1 min. The TEM grid was viewed using JEM-1400 Plus TEM operated at 80–100 kV (JEOL, Peabody, MA, USA). For SEM, 5 μL NDMV suspension was placed onto silicon chips and dehydrated with series of solvents for 5 min, each with 100% acetone, 100% ethanol and deionized water. Samples were subjected to critical point drying and mounted on an SEM stub which was sputter coated with 40 nm gold-palladium alloy and observed by JSM-7610F SEM operated at 25–50 kV (JEOL, Peabody, MA, USA).
4
Exosome Visualization by TEM
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For transmission electron microscopy (TEM), approximately 10 µl aliquot of exosomes was placed on a copper mesh and incubated at 20 °C for 10 min. After washing with sterile distilled water, the exosomes were contrasted using uranyl-oxalate solution for 1 min. Further, the sample was dried for 2 min under incandescent light. The copper mesh was examined at 100 kV using a JEOL JEM-1400Plus TEM (JEOL, Peabody, USA) according to the manufacturer’s instructions.
5
Characterization of SBL Microparticles
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SBL microparticles were analysed on a JEOL JEM-1400 Plus TEM operating at an acceleration voltage of 120 kV. The diluted particle dispersion (c = 0.3 g L−1) was dropped on a carbon-coated copper grid. The sample was blotted with a filter paper after 30 s and the grid was dried for at least 12 h. The samples were stained with OsO4 for 1 h before analysis. The TEM images were processed with ImageJ (version 1.53c).
6
Visualizing Phage Particles via TEM
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Purified phage particles at a concentration of 109 PFU/mL were deposited on 300 mesh carbon-coated copper grids (Agar Scientific, United Kingdom) and negatively stained with 1% uranyl acetate (pH 4) as follows: the surface of the copper grids were ionized for 2 min immediately prior to sample deposition. 5 μL of phage lysate were spotted on the surface of the grid and allowed to stand for 1 min. This was followed by 30 sec negative staining and subsequent air drying of the grid. Visualization was performed using a JEOL JEM-1400Plus TEM, operated at 120 kV (pixel size = 0.1 nm), equipped with a Gatan OneView 4K camera with automatic drift correction.
7
Visualizing Yeast Cell Structure with SEM and TEM
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Preparation of isolated yeast after growing in PR extract according to Afrikian et al., (1973). Scanning Electron Microscopes (SEM): The effect of PR extract on structure of vegetation yeast cells after growing in PR extract according to Bozzola and Russell (1999) . Examination of the yeast cells was carried out by using JEOL JSM-7610F Scanning Electron Microscope at magnification of 900×, 8000×, 10,000×, 16,000× and 20,000×.
Transmission Electron Microscopes (TEM): The Effect of PR extract on structure of vegetation yeast cells after growing in PR extract (Bozzola and Russell 1999 ). Examination of the yeast cells was carried out by using JEOL JEM-1400plus TEM at magnification of 20000×, 30000×, 40000×, 50000× and 60000×.
Transmission Electron Microscopes (TEM): The Effect of PR extract on structure of vegetation yeast cells after growing in PR extract (Bozzola and Russell 1999 ). Examination of the yeast cells was carried out by using JEOL JEM-1400plus TEM at magnification of 20000×, 30000×, 40000×, 50000× and 60000×.
8
Ultrastructural Characterization of Xylem Pit Membrane
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The middle parts of inflorescence stem segments were collected immediately after Cavitron measurements and fixed in Karnovsky’s fixative (Karnovsky, 1965 ). Subsequently, the samples were washed in 0.1 M cacodylate buffer and post-fixed with 1% buffered osmium tetroxide. The samples were then prepared for semi-thin and ultra-thin sectioning according to the protocol described in Thonglim et al. (2020) , and were observed with a JEM-1400 Plus TEM (JEOL, Tokyo, Japan) with an 11 megapixel digital camera (Quemesa, Olympus). TEM observations were conducted to measure the intervessel pit membrane thickness and the pit chamber depth (Supplementary Table S2 ).
9
TEM Analysis of Cu2+ Impact on Cell Morphology
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A transmission electron microscope (TEM) was employed to check whether the cell membrane or morphology was changed in the presence of Cu2+. Briefly, the mating system was exposed to Cu2+ at 5 and 0 μmol/L. After incubating for 18 h, the mixed samples were collected, fixed, dehydrated, filtered, and mounted. Ultrathin sections (50–100 nm) of each sample were applied on TEM copper grids and obtained using a JEM-1400PLUS TEM (Jeol, Japan) at 80 kV.
10
Ultrastructural Analysis of Optic Nerve
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Rats were sacrificed after 28 days, and three optic nerves were removed from each group. The optic nerve samples were fixed in 3% glutaraldehyde and postfixed in 1% osmium tetroxide (Leica Company, Germany). After dehydration by gradient acetone dehydration, the optic nerve samples were permeated in Epon 812 epoxy resin (Beijing Keyi, Beijing, China). Then, the permeated samples were embedded in flat molds and heating polymerized for embedded blocks. Ultrathin sections (50 nm) were created with an ultramicrotome and double-stained with Reynolds’s lead citrate and 0.5% aqueous uranyl acetate (Beijing Keyi, Beijing, China). The microscopic examination was performed with a JEM-1400PLUS TEM (JEOL, Tokyo, Japan), and the images were analyzed with OlyVIA software (version 2.8).
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