Blood was drawn from participants concurrent with CMR, and samples were processed for serum and plasma on the same day using standard protocols and stored at − 80 °C until assays were performed. Biomarkers of inflammation (interleukin-6 [IL-6], soluble cluster of differentiation 14 [sCD14], galectin-3), ventricular and cellular stress (N-terminal prohormone of brain natriuretic peptide [NT-proBNP], growth differentiation factor-15 [GDF-15]), tissue remodeling (tissue inhibitor of metalloproteinase-2 [TIMP-2], matrix metalloproteinase-2 [MMP-2]) and tissue injury (high-sensitivity troponin I [hsTnI]) were measured in serum and plasma at central laboratories. Serum levels of IL-6 were measured by electrochemiluminescence (Meso Scale Discovery V-PLEX), and the following markers were measured by enzyme-linked immunosorbent assay: sCD14 (R&D Systems Quantikine), GDF-15 (R&D Systems Quantikine), TIMP-2 (R&D Systems Quantikine), MMP-2 (R&D Systems Quantikine), and NT-proBNP (Abbott Architect i2000sR) in serum, and galectin-3 (Abbott Architect i2000sR) and hsTnI (Abbott Architect i2000sR) in plasma.
I2000sr
Manufactured by Abbott
Sourced in United States, Germany, China
The I2000SR is an automated immunoassay analyzer designed for use in clinical laboratories. It is capable of performing a variety of immunoassay tests, including tests for hormones, drugs, and infectious diseases. The I2000SR utilizes chemiluminescent technology to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Au680, by Beckman Coulter (9 mentions)
C16000, by Abbott (3 mentions)
Architect hbsag and hbeag reagent kits, by Abbott (2 mentions)
Polystyrene sample cups, by Abbott (2 mentions)
Immulite 2000, by Siemens (2 mentions)
Xe 2100, by Sysmex (2 mentions)
Au 680 analyzer, by Abbott (2 mentions)
Sars cov 2 igg assay, by Abbott (2 mentions)
Advia centaur xp, by Siemens (2 mentions)
Array 360 system, by Beckman Coulter (1 mentions)
Architect havab igg, by Abbott (1 mentions)
Accu chek active, by Roche (1 mentions)
Automatic biochemical analyzer, by Roche (1 mentions)
Advia 1800 chemistry system, by Siemens (1 mentions)
Immulite 2000 xpi, by Siemens (1 mentions)
C8000, by Abbott (1 mentions)
Bn 2, by Siemens (1 mentions)
Cell dyn 3700, by Abbott (1 mentions)
Au5821, by Beckman Coulter (1 mentions)
I1000sr, by Abbott (1 mentions)
57 protocols using i2000sr
1
Biomarker Assessment in Cardiovascular Health
2
Hormonal and Metabolic Profiles in Patients
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We recorded the clinical information of all patients and normal controls, including age, weight, height, waist circumference, and hip circumference. Body mass index was calculated as BMI = weight (kg)/height2 (m2). Waist-to-hip ratio (WHR) was calculated as waist circumference/hip circumference. Peripheral blood (3-5 mL) was collected from patients and controls. The serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured using a chemiluminescence immunoassay analyzer (i2000SR, Abbott). Fasting blood glucose (FBG) was measured using hexokinase method (AU5821, Beckman, Germany). Fasting insulin (FNS) was detected by chemiluminescence method (i2000SR, Abbott). The formula for calculating the insulin resistance index (HOMA-IR) is as follows: [HOMA − IR = fasting insulin (mIU/L) × fasting blood glucose (mmol/L)/22.5].
3
Antiviral Efficacy of Compounds against HBV
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Cultures were treated with 3 or 9 consecutive daily doses of the compounds. The medium containing compounds was changed every 3 days. Lamivudine was used as the antiviral positive control. The solvent used in the drug preparation was used as the DMSO negative control. At day 3 or day 9, the cell culture medium was centrifuged at 3000 rpm for 20 min to remove debris or intact cells before analysis. The collected culture medium contained HBV DNA, HBV surface antigen (HBsAg) and HBeAg. The HBV DNA was extracted and detected with qPCR using the kit (Acon, Hangzhou, China) following the manufacturer’s protocols. The expression levels of HBsAg and HBeAg were determined using Abbott i2000SR with Architect HBsAg and HBeAg Reagent kits (Abbott Diagnostics, Abbott Park, IL, USA), respectively according to the manufacturer’s protocols [29 (link)].
4
HIV Screening with ELISA and Chemiluminescence
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All samples were screened by using an HIV antibody/antigen enzyme-linked immunosorbent assay (ELISA) kit (Wantai Bio-Pharm) and HIV Ag/Ab Combo Reagent Kit (Abbott Germany Limited Partnership, USA). The protocol was performed according to the manufacturer’s instructions. In the ELISA test, 20μL of biotin-conjugate reagent and 100μL of the sample were added to a microwell strip pre-coated with recombinant HIV antigen and anti-p24 monoclonal antibody. The ELISA plate was incubated for 60 minutes at 37°C, followed by five washes. Next, 100 μL enzyme-labeled Ab/Ag was added for further incubation at 37°C for 30 minutes. After five washes, the substrate was added to the well for 30 minutes to allow for colour development, and the reaction was terminated by adding a stop solution. The optical density value of each well was measured using a microplate reader at a single wavelength of 450 nm or a dual wavelength of 450/630 nm. Chemiluminescence particle immunoassay was used to qualitatively detect the HIV p24 antigen and HIV-1/2 antibody (Abbott i2000SR, Abbott Laboratories, USA).
5
Comprehensive Metabolic Profile in Patients
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Fasting venous blood samples were collected from all patients. Hemoglobin and the red cell distribution width (RDW) were measured by a Japanese SYSMEX XN-1000 automatic blood routine detector. An ABBOTT Architect C16000 Automatic dry chemical analyzer was used to detect the serum albumin (sAlb), prealbumin (pre-Alb), glucose, serum creatinine (sCr), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) concentrations. The adjusted calcium (Ca) levels were calculated with the following formula. Adjusted Ca = serum Ca + 0.02×(40-sAlb). An ABBOTT Architect I2000SR automatic chemiluminescence immunoassay was used to measure intact parathyroid hormone. The high-sensitivity C-reactive protein level was determined with a Beckman Array 360 System using scattering rate turbidimetry. The serum IL-6 level was measured by ELISA.
6
Automated Immunoassay for HAV Antibody Detection
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Anti-HAV IgG was determined using the automated chemiluminescent microparticle immunoassay on the ARCHITECT i2000SR instrument (ARCHITECT HAVab-IgG, Abbott, Wiesbaden, Germany) according to the manufacturer’s instructions. The sensitivity and specificity of the test were > 98% and > 99.17%, respectively. Samples with the signal-to-cut-off (S/CO) ratio of > 1.00 were considered HAV antibody-positive by the automated assay, while samples with S/CO ratio < 1.00 were considered negative.
7
Evaluation of ARCHITECT HIV Ag/Ab Assay
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The ARCHITECT HIV Ag/Ab Combo assay was performed according to the manufacturer's instructions without modification. Plasma specimens were centrifuged for ten minutes at 8,000 rpm, transferred to polystyrene sample cups (Abbott Laboratories) in a total volume of 200µL, and then loaded onto the ARCHITECT i2000SR. The specimens were tested in singlicate due to volume limitations. The chemiluminescent signal, as measured by the instrument, is reported as a S/Co ratio of the relative light units (RLU) of the test sample to the RLU of the cutoff determined from an ARCHITECT calibrator. In addition to the kit controls, high and low external HIV-1 positive controls were included in each run. The controls were obtained from a proficiency testing panel derived from HIV-1-seropositive serum [22] (link). The high and low positive controls are consistent with long-term and recent infection, respectively, as measured by a previously characterized HIV incidence assay [23] (link).
8
Seroprevalence of COVID-19 Antibodies
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Eligible participants were interviewed to collect information about sociodemographic details, symptoms suggestive of COVID-19 since March 1, 2020 (eg, fever, cough, shortness of breath, sore throat, new loss of taste or smell, fatigue), exposure history to laboratory-confirmed COVID-19 cases, and history of COVID-19 illness using the Open Data Kit mobile phone application. 3–5 mL of venous blood was collected from each participant, and centrifuged serum samples were transported to ICMR National Institute of Epidemiology, Chennai under cold chain.
Participant serum samples were tested for the presence of SARS-CoV-2 specific IgG antibodies on the Abbott Architect i2000SR automated analyser using the Abbott SARS-CoV-2 IgG assay (Abbott Park, IL, USA) as per the manufacturer's instructions. This assay detects IgG antibodies against the SARS-CoV-2 nucleocapsid protein, and has a sensitivity of 100·0% and specificity of 99·6%.5 The assay was calibrated with positive and negative quality controls before analyses. Assay results higher than or equal to the cutoff index value of 1·4 were interpreted as positive for SARS-CoV-2 antibodies. As a part of quality control, 10% of positive serum samples and an equal number of negative serum samples were re-tested using the same assay.
Participant serum samples were tested for the presence of SARS-CoV-2 specific IgG antibodies on the Abbott Architect i2000SR automated analyser using the Abbott SARS-CoV-2 IgG assay (Abbott Park, IL, USA) as per the manufacturer's instructions. This assay detects IgG antibodies against the SARS-CoV-2 nucleocapsid protein, and has a sensitivity of 100·0% and specificity of 99·6%.5 The assay was calibrated with positive and negative quality controls before analyses. Assay results higher than or equal to the cutoff index value of 1·4 were interpreted as positive for SARS-CoV-2 antibodies. As a part of quality control, 10% of positive serum samples and an equal number of negative serum samples were re-tested using the same assay.
9
Biochemical Markers for Metabolic Health
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Blood samples were collected from all participants after fasting for more than 8 hours and immediately centrifuged (2000 rpm for 15 min) at room temperature. Then, the samples were sent to a central laboratory certified by the College of American Pathologists. Biochemical indexes such as Cr, LDL, TG, HDL, and TC were measured using a BECKMAN COULTER AU 680 with the original kit. HbA1c and insulin were evaluated using an MQ-2000PT (Medconn Technology, Shanghai, China) and Abbott i2000 SR, respectively. Serum 25(OH)D was measured using a Siemens ADVIA Centaur XP with the original kit. The intra- and inter-assay coefficients of variance were 6.25% and 8.33%, respectively.
10
Fasting Blood Sample Analysis Protocol
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Blood samples were obtained after fasting for at least 8 h; the samples were immediately centrifuged (2000 rpm for 15 min) at room temperature, and stored at − 20 °C when collected and shipped by air in dry ice within 2–4 h of collection to a central laboratory certified by the College of American Pathologists. All plasma and serum samples were frozen at − 80 °C after laboratory testing. Biochemical indexes, including the fasting plasma glucose (FPG), total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), alanine aminotransferase (ALT) were analyzed by Beckman Coulter AU680 (Bera, USA). Serum 25(OH) D (SIEMENS ADVIA Centaur XP, Siemens, Germany) and insulin (Abbott i2000 SR, Chicago, USA) were measured using the chemiluminescence method. Glycated hemoglobin (HbA1c) was detected using high-performance liquid chromatography (HPLC) with MQ-2000PT (Medconn Technology, Shanghai, China) using a commercial reagent (HuaChen biological reagent co., LTD, Shanghai, China).
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