Anti survivin 71g4b7

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-survivin (71G4B7) is a mouse monoclonal antibody that recognizes the survivin protein, a member of the inhibitor of apoptosis (IAP) family. Survivin is expressed during cell division and plays a role in regulating cell cycle and apoptosis. The Anti-survivin (71G4B7) antibody can be used to detect and study the expression of survivin in various biological samples.

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Close spelling variants of this product

Anti-Survivin (clone 71G4B7, Survivin (71G4B7), Anti-survivin Survivin

4 protocols using anti survivin 71g4b7

Protein Solubilization and Immunoblotting

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Protein solubilization and immunoblotting were conducted as previously described [15 (link)]. The following commercially available antibodies were used: anti-TAZ (V386) (#4883; Cell Signaling Technology, Beverly, MA, USA), anti-survivin (71G4B7) (#2808; Cell Signaling Technology), anti-p53 (sc-126; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p21 (F-5, Santa Cruz Biotechnology), and anti-β-actin-HRP (C4; Santa Cruz Biotechnology).

Miyajima C., Hayakawa Y., Inoue Y., Nagasaka M, & Hayashi H. (2022). HMG-CoA Reductase Inhibitor Statins Activate the Transcriptional Activity of p53 by Regulating the Expression of TAZ. Pharmaceuticals, 15(8), 1015.

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Apoptosis Regulators Expression Analysis

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Anti-BIRC6 (Novus Biologicals, #NB110-40730) [28 (link)], anti-survivin (71G4B7) (Cell Signaling, #2808) [49 (link)]; anti-XIAP (H-202) (#sc-11426, Santa Cruz Biotechnology, Santa Cruz, CA) [50 (link)]. cIAP-1/HIAP-2 antibody (R&D Systems #MAB8181) for IHC, anti-cIAP1 (D5G9) (#7065, Cell Signaling Technology, Danvers, MA) for Western Blotting. The same antibodies were used for immunohistochemistry and Western blotting unless otherwise indicated.

Iris Luk S.U., Xue H., Cheng H., Lin D., Gout P.W., Fazli L., Collins C.C., Gleave M.E, & Wang Y. (2014). The BIRC6 gene as a novel target for therapy of prostate cancer: dual targeting of inhibitors of apoptosis. Oncotarget, 5(16), 6896-6908.

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Antibodies for Western Blot and Immunofluorescence

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The antibodies used in this study were obtained from the following sources: anti-survivin (71G4B7, Cat#2808), anti-XIAP (3B6, Cat#2045), anti-phospho-JAK1 (Y1034/1035; Cat#3331), anti-phospho-STAT3 (Y705; D3A7, Cat#9145), anti-cleaved PARP (Cat#5625T), anti-cleaved caspase 3 (Cat#9664) and anti-phospho-JAK2 (Y1007/1008; Cat#3771) from Cell Signaling Technology Inc. (Beverly, MA, USA); anti-actin (I-19, Cat#sc-1616), anti-GAPDH (0411, Cat#sc-47724), anti-JAK1 (B-3, Cat#376996), anti-phospho-STAT3 (Y705; B-7, Cat#sc-8059), anti-STAT3 (F-2, Cat#sc-8019), from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA); anti-vinculin (Cat#V-4505) from Sigma-Aldrich. Antibody binding was detected with donkey anti-mouse IgG-HRP (Cat#A16017), donkey anti-rabbit IgG-HRP (Cat#A16029) and donkey anti-goat IgG-HRP (Cat#A15999) from Thermo Fisher Scientific Inc. (Waltham, MA, USA); Alexa Fluor™ 488-conjugated donkey anti-mouse (Cat#A31572, Molecular Probes, Eugene, OR, USA) was used for antibody binding detection in immunofluorescence assays.

Martínez-García D., Pérez-Hernández M., Korrodi-Gregório L., Quesada R., Ramos R., Baixeras N., Pérez-Tomás R, & Soto-Cerrato V. (2019). The Natural-Based Antitumor Compound T21 Decreases Survivin Levels through Potent STAT3 Inhibition in Lung Cancer Models. Biomolecules, 9(8), 361.

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Western Blotting for Protein Expression

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Western blotting for the expression of E-cadherin, vimentin, CD44, LC3A, MET, ATG5, total EGFR, phosphorylated EGFR, poly(ADP-ribose) polymerase-1 (PARP-1), γH2AX, total AKT, phospho-AKT, total ERK1/2, phospho-ERK1/2, Mcl-1, Bim, and β-actin was performed as described previously. 6, 13 Additional primary antibodies for immunoblotting were anti-ZEB1 (HPA027524; 1:1000 dilution; Atlas Antibodies, Stockholm, Sweden), anti-ATG7 (D12B11; 1:1000 dilution; Cell Signaling), anti-Pin1 (1:1000 dilution; Cell Signaling), anti-Survivin (71G4B7; 1:1000 dilution; Cell Signaling), and anti-Puma (EP512Y; 1:2000 dilution; Abcam Japan, Tokyo, Japan). Band intensity levels on X-ray films were normalized to β-actin using the Image J software (National Institutes of Health, Bethesda, MD, USA).

Sakuma Y., Nishikiori H., Hirai S., Yamaguchi M., Yamada G., Watanabe A., Hasegawa T., Kojima T., Niki T, & Takahashi H. (2016). Prolyl isomerase Pin1 promotes survival in EGFR-mutant lung adenocarcinoma cells with an epithelial-mesenchymal transition phenotype. Laboratory investigation; a journal of technical methods and pathology, 96(4).

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