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Axio wide field microscope

Manufactured by Zeiss
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The Axio wide-field microscope is a versatile laboratory instrument designed for general microscopy applications. It provides a clear, high-quality image of specimens under observation. The microscope's core function is to magnify and display samples for detailed analysis and examination.

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Lab products found in correlation

Mirax scan, by Zeiss (3 mentions) Discovery xt system, by Roche (2 mentions)

Spelling variants of this product

Axio microscope (122 citations) Axio Observer.Z1 inverted wide-field microscope (3 citations) Wide-field microscopes (2 citations) Axio Imager 2 wide-field microscope (2 citations) Axio Imager M2 Plus Wide Field Fluorescence Microscope (2 citations)

3 protocols using axio wide field microscope

1

IHC Analysis of Ki67 and RFP

Cited 1 time
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Immunohistochemistry (IHC) was performed as before[22 (link)]. Ki67 (Abcam, #ab15580) and RFP (Rockland, #600–401-379) staining was performed by the institutional Molecular Cytology Core using a Discovery XT system (Ventana Medical Systems, Roche). Slides were either imaged on an Axio wide-field microscope (Zeiss) or scanned with MIRAX scan (Zeiss) and analyzed with Pannoramic Viewer. Ki67 was quantified by manually counting stained nuclei in the field size as indicated. N = 7 and 9 biological replicates for the V558Δ and V558Δ;V653A groups, respectively.
Zhang J.Q., Bosbach B., Loo J.K., Vitiello G.A., Zeng S., Seifert A.M., Medina B.D., Param N.J., Maltbaek J.H., Rossi F., Antonescu C.R., Besmer P, & DeMatteo R.P. (2020). The V654A second-site KIT mutation increases tumor oncogenesis and STAT activation in a mouse model of gastrointestinal stromal tumor. Oncogene, 39(49), 7153-7165.
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2

IHC Analysis of Ki67 and RFP

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry (IHC) was performed as before[22 (link)]. Ki67 (Abcam, #ab15580) and RFP (Rockland, #600–401-379) staining was performed by the institutional Molecular Cytology Core using a Discovery XT system (Ventana Medical Systems, Roche). Slides were either imaged on an Axio wide-field microscope (Zeiss) or scanned with MIRAX scan (Zeiss) and analyzed with Pannoramic Viewer. Ki67 was quantified by manually counting stained nuclei in the field size as indicated. N = 7 and 9 biological replicates for the V558Δ and V558Δ;V653A groups, respectively.
Zhang J.Q., Bosbach B., Loo J.K., Vitiello G.A., Zeng S., Seifert A.M., Medina B.D., Param N.J., Maltbaek J.H., Rossi F., Antonescu C.R., Besmer P, & DeMatteo R.P. (2020). The V654A second-site KIT mutation increases tumor oncogenesis and STAT activation in a mouse model of gastrointestinal stromal tumor. Oncogene, 39(49), 7153-7165.
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3

Quantifying Tissue Immunohistochemistry

Cited 1 time
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Immunohistochemistry (IHC) was performed as before (11 (link)). Ki67 (Vector Laboratories) and CD40 (Polyclonal C-20; Santa Cruz) staining of mouse and human tissues, respectively, was performed by the institutional Molecular Cytology Core. Slides were either imaged on an Axio wide-field microscope (Zeiss) or scanned with MIRAX scan (Zeiss) and analyzed with Pannoramic Viewer by laboratory member. Ki67 was quantified by manually counting stained nuclei in the field size, as indicated. Trichrome staining was quantified using ImageJ software’s Colour Deconvolution Plug-In (29 (link)).
Zhang J.Q., Zeng S., Vitiello G.A., Seifert A.M., Medina B.D., Beckman M.J., Loo J.K., Santamaria-Barria J., Maltbaek J.H., Param N.J., Moral J.A., Zhao J.N., Balachandran V., Rossi F., Antonescu C.R, & DeMatteo R.P. (2018). Macrophages and CD8+ T cells mediate the antitumor efficacy of combined CD40 ligation and imatinib therapy in gastrointestinal stromal tumors. Cancer immunology research, 6(4), 434-447.
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