Hamsters were euthanized with carbon dioxide (CO2) inhalation and necropsy was performed. The lungs were inspected for gross lesions and representative portions of the lungs were collected in 10% buffered formalin for histology. Formalin-fixed tissues were processed per a standard protocol, 4 μm-thick sections were cut and stained with hematoxylin and eosin (HE). The slides were imaged in a digital scanner (Leica Aperio LV1). Lung sections were examined under light microscopy using an Olympus CX43 microscope for the extent of inflammation, size of inflammatory foci, and changes in alveoli, alveolar septa, airways, and blood vessels. The blinded tissue sections were semi-quantitatively scored for pathological lesions. The criteria for histopathology scoring are presented in Supplementary Table 2 .
Aperio lv1
Manufactured by Leica camera
The Aperio LV1 is a high-performance digital slide scanner designed for use in pathology laboratories. It is capable of capturing whole-slide images at high resolution, enabling detailed analysis and collaboration. The Aperio LV1 is a precision instrument that provides consistent and reliable scanning performance.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using aperio lv1
1
Histological Lung Examination in Hamsters
2
Histological Assessment of MRSA-Induced Lung Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were euthanized seven days after MRSA super-infection and the lungs were removed for histological analyses. Paraffin-embedded tissues were sectioned to a thickness of 5 µm and stained with hematoxylin and eosin (H&E) using standard methods. Whole mount H&E-stained lung tissues were scanned using Leica Aperio LV1 scanner and software, and semi quantitatively assessed at low-power (40×) for the proportion of parenchyma with alveoli containing intraluminal material (proteinaceous exudate and/or fibrin) in the background of interstitial expansion and inflammation. Each lung was scored by the relative amount of abnormal tissue as follows: normal-0, 1-25%-1, 26-50%-2, 51-75%-3, >76%-4 (15 (link)). Digital images were generated using Leica Biosystems Aperio ImageScope 12.
3
Histological Analysis of Mouse Lungs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The freshly collected lungs of the mice were fixed in formalin for 1 week. After being treated including dehydration, clearing, infiltration, and embedding in an automatic biological tissue dehydrator, the tissues were embedded in paraffin wax. The tissues were cut into 4 μm thick and taken to perform hematoxylin-eosin (H&E) staining using a glass slide automatic dyeing machine. The H&E sections were scanned using a Leica Aperio LV1.
4
Hamster Lung Histopathology Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hamsters were euthanized with ketamine/xylazine injection and necropsy was performed. The lungs were inspected for gross lesions and representative portions of the lungs were collected in 10% buffered formalin for histology. Formalin-fixed tissues were processed per a standard protocol, 4 μm-thick sections were cut and stained with hematoxylin and eosin (HE). The slides were imaged in a digital scanner (Leica Aperio LV1). Lung sections were examined under light microscopy using an Olympus CX43 microscope for the extent of inflammation, size of inflammatory foci, and changes in alveoli, alveolar septa, airways, and blood vessels. The blinded tissue sections were semi-quantitatively scored for pathological lesions.
5
Histopathological Analysis of Hamster Lungs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following euthanasia of hamsters with ketamine/xylazine injection, necropsy was performed, and lungs were inspected for gross lesions. A representative specimen of lungs (right lower lobe) was collected in 10% buffered formalin for histological examination. Formalin-fixed tissues were processed per a standard protocol for histological analysis, and 4 μm-thick sections were cut and stained with hematoxylin and eosin (HE). Lung sections were examined under light microscopy using an Olympus CX43 microscope for the extent of inflammation, size of inflammatory foci, and changes in alveoli, alveolar septa, airways, and blood vessels. The slides were imaged in a digital scanner (Leica Aperio LV1). The blinded tissue sections were semi-quantitatively scored for pathological lesions using the criteria described in Supplementary Table 3 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!