Intracellular proteins in HeLa cells or hDFs treated with N-CM or H-CM for 24 h were analyzed with a Signaling explorer antibody array (Full Moon BioSystems, Sunnyvale, CA, USA). Briefly, 50 μg of protein sample was added to a microarray slide with 1,358 antibodies and the slide was treated with Cy3-streptavidin (GE Healthcare, Chalfont St. Giles, UK). The slide was washed with washing solution and rinsed with Milli-Q grade water. For data acquisition and analysis, the array slide was scanned with a GenePix 4100A scanner and the image was quantified with GenePix 7.0 Software. The data were analyzed using Genowiz 4.0TM) and protein information was annotated using the UniProt DB. Up- and down-regulated proteins (>1.5 fold) were identified and analyzed with tools in DAVID (p<0.01).
Signaling explorer antibody array
Manufactured by Full Moon BioSystems
Sourced in United States
The Signaling Explorer Antibody Array is a comprehensive protein analysis tool designed to simultaneously detect and quantify the expression levels of a wide range of signaling proteins. The array features a collection of high-quality antibodies that target key signaling molecules, enabling researchers to gain a broader understanding of cellular signaling pathways and networks.
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Lab products found in correlation
2 protocols using signaling explorer antibody array
1
Signaling Pathway Profiling in HeLa and hDF Cells
2
Comprehensive Signaling Pathway Analysis
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Mouse cavernosum tissues from age-matched control and diabetic mice intracavernous injected with HBS (H), PC-NVs, or Lcn2-knockdown (KD) PC-NVs were used for Signaling Explorer Antibody Array (Fullmoon Biosystems, Sunnyvale, CA, USA), performed according to the manufacturer's instructions. Assay consists of 1358 unique antibodies covering 20 cell-signaling pathways, was performed as a custom service by E-Biogen (Ebiogen Inc.).
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