For tissue staining, frozen sections of vagina, spleen and lung collected from various experimental groups were stained
with the Ab (anti-CD4 [H129.19]) in a previously described procedure29 (link).
Likewise, FITC, CD138, B220, CD31, VCAM-1, IgG2b, IgG2c, total IgG and HSV-2 antigens were detected using Alexa Fluor
647-conjugated anti-FITC Ab, biotin-conjugated anti-mouse CD138 Ab (281–2), PE-conjugated anti-mouse B220 Ab
(RA3–6B2), biotin-conjugated anti-mouse CD31 Ab (390), biotin-conjugated anti-VCAM-1 Ab (429/MVCAM.A), HRP-conjugated
IgG2b&IgG2c (SouthernBiotech), Alexa Fluor 568-conjugated anti-mouse IgG (H+L) (Invitrogen) and FITC-conjugated pAbs against
HSV 1 and 2 (ViroStat). Stained slides were washed and incubated with DAPI and mounted with Fluoromount-G (SouthernBiotech).
Images were captured using a 10x or 40x objective lens from fluorescence microscopy (BX51; Olympus).
with the Ab (anti-CD4 [H129.19]) in a previously described procedure29 (link).
Likewise, FITC, CD138, B220, CD31, VCAM-1, IgG2b, IgG2c, total IgG and HSV-2 antigens were detected using Alexa Fluor
647-conjugated anti-FITC Ab, biotin-conjugated anti-mouse CD138 Ab (281–2), PE-conjugated anti-mouse B220 Ab
(RA3–6B2), biotin-conjugated anti-mouse CD31 Ab (390), biotin-conjugated anti-VCAM-1 Ab (429/MVCAM.A), HRP-conjugated
IgG2b&IgG2c (SouthernBiotech), Alexa Fluor 568-conjugated anti-mouse IgG (H+L) (Invitrogen) and FITC-conjugated pAbs against
HSV 1 and 2 (ViroStat). Stained slides were washed and incubated with DAPI and mounted with Fluoromount-G (SouthernBiotech).
Images were captured using a 10x or 40x objective lens from fluorescence microscopy (BX51; Olympus).