Bcl xl h 62

Manufactured by Santa Cruz Biotechnology

Bcl xL (H-62) is a protein that belongs to the Bcl-2 family of proteins. It functions as an anti-apoptotic protein, which means it plays a role in inhibiting programmed cell death or apoptosis.

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Bcl-xL

2 protocols using bcl xl h 62

Comprehensive Protein Analysis by Western Blot

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Total protein was resolved by SDS-PAGE (10–15% Tris-Glycine), transferred onto Hybond nitrocellulose membrane (Amersham biosciences) and probed with antibodies specific for Phospho-STAT3 (S727) (ab32143, abcam), Phospho-STAT3 (Y705) (9131, Cell Signalling Technology (CST)), Total STAT3 (C-20: sc-482, Santa Cruz Biotechnology), Phospho-STAT5 (Y694) (9314, CST), Phospho-JAK2 (Y1007/1008) (3776, CST), Total JAK2 (3230, CST), involucrin (SY5, Santa Cruz Biotechnology), HPV18 E6 (G-7, Santa Cruz Biotechnology), HPV18 E7 (8E2, Abcam (ab100953), HPV 16/18 E6 (C1P5, Santa Cruz Biotechnology), HPV 16 E7 (ED17, Santa Cruz Biotechnology), Phospho-ERK1/2 (Thr202/Tyr204) (43705, CST), Phospho-JNK (Thr183/Tyr185) 4668, CST), Phospho-p38 (Thr180/Tyr182) (9211, CST), Bcl xL (H-62, Santa Cruz Biotechnology), Cyclin D1 (A-12, Santa Cruz Biotechnology) p53 (FL-393, Santa Cruz Biotechnology), p21 (2947, CST), FLAG (F3165, Sigma), GFP (B-2: sc-9996, Santa Cruz Biotechnology) and GAPDH (G-9, Santa Cruz Biotechnology). Western blots were visualized with species-specific HRP conjugated secondary antibodies (Sigma) and ECL (Thermo/Pierce).

Morgan E.L., Wasson C.W., Hanson L., Kealy D., Pentland I., McGuire V., Scarpini C., Coleman N., Arthur J.S., Parish J.L., Roberts S, & Macdonald A. (2018). STAT3 activation by E6 is essential for the differentiation-dependent HPV18 life cycle. PLoS Pathogens, 14(4), e1006975.

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Immunoblotting Primary Antibody Optimization

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Primary antibodies for immunoblotting were purchased from commercial sources: actin (horseradish peroxidase; I-19), GAPDH horseradish peroxidase (V18), SHP-1 (C19), PDGF-β (N30), and Bcl-xL (H-62) from Santa Cruz Biotechnology Inc (Dallas, TX); protein kinase B (Akt), phospho-Akt (D9E), phospho-PDGFR-β (Y1009), PDGFR-β, phospho-ERK (extracellular signal-regulated kinase), ERK and secondary antibody of anti-rabbit and anti-mouse

Mercier C., Brazeau T., Lamoureux J., Boisvert E., Robillard S., Breton V., Paré M., Guay A., Lizotte F., Despatis M.A, & Geraldes P. (2021). Diabetes Impaired Ischemia-Induced PDGF (Platelet-Derived Growth Factor) Signaling Actions and Vessel Formation Through the Activation of Scr Homology 2-Containing Phosphatase-1. Arteriosclerosis, thrombosis, and vascular biology, 41(9).

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