Human pancreatic cancer cell lines AsPC-1, Panc-1, Capan-1, and Mia PaCa-2 were obtained from ATCC (Manassas, VA), the murine cell line Pan02 was obtained from the DCTD tumor repository maintained by the NCI at Frederick. C5LM2 is a variant of Panc1 developed in our laboratory that was generated through 2 passages of growth in vivo and culture of liver metastases and has been characterized previously (11 (link)). C5LM2, AsPC-1, Panc-1, Pan02, and Mia PaCa-2 lines were grown in DMEM, Capan-1 in was grown in IMDM, all cell lines were grown in a humidified atmosphere with 5% CO2, at 37°C, and have been DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC and were confirmed to be free of mycoplasma (e-Myco kit, Boca Scientific).
Pan02
Manufactured by American Type Culture Collection
Sourced in United States
The Pan02 is a cell line derived from a pancreatic ductal adenocarcinoma, a type of pancreatic cancer, in C57BL/6 mice. It is a widely used model for studying pancreatic cancer and can be used for in vitro and in vivo research.
Automatically generated - may contain errors
Lab products found in correlation
Panc 1, by American Type Culture Collection (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Mia paca 2, by American Type Culture Collection (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Bxpc 3, by American Type Culture Collection (2 mentions)
Hek293ft, by Thermo Fisher Scientific (2 mentions)
Aspc 1, by American Type Culture Collection (2 mentions)
Pan02, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Pnifty tlr signaling reporter plasmid luciferase, by InvivoGen (2 mentions)
Fugene hd transfection reagent, by Promega (2 mentions)
Renca, by American Type Culture Collection (2 mentions)
Bs c 1, by American Type Culture Collection (2 mentions)
Powerplex 1.2 kit, by Promega (1 mentions)
Capan 1, by American Type Culture Collection (1 mentions)
Mia paca 2, by Promega (1 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Rpmi 1640, by Corning (1 mentions)
16 protocols using pan02
1
Characterization of Human and Murine Pancreatic Cancer Cell Lines
2
Murine and Human Cancer Cell Lines
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Murine B16F10 murine melanoma cells were provided by Dr. Mary Jo Turk at the Geisel School of Medicine at Dartmouth College (Hanover, NH, USA). C26 cell was obtained from the National Cancer Institute. 4T1 breast and LLC lung cancer cells were provided by Dr. Chandan Guha at the Albert Einstein College of Medicine (Bronx, NY, USA). Pan02 cells were obtained from ATCC (Manassas, VA, USA). B16F10 and C26 cells were cultured in Dulbecco Modified Eagle’s medium (DMEM; Corning, Oneonta, NY, USA) containing 10% fetal bovine serum (FBS, Atlanta Biologicals Inc., Atlanta, GA, USA) and 1% streptomycin/penicillin (Gibco, Life Technologies, Waltham, MA, USA). Colon carcinoma (C26) and murine 4T1 breast cancer cell lines, from ATCC, were grown in RPMI 1640 (Corning) containing 10% FBS and 1% streptomycin/penicillin. The ATCC murine pancreatic cell line (Pan02) was grown in Eagle’s Minimum Essential Medium (EMEM, ATCC) containing 10% FBS and 1% streptomycin/penicillin. Human male malignant melanoma tissues, colorectal adenocarcinoma, and normal colon tissues were provided by the Cooperative Human Tissue Network, Southern Division, University of Alabama at Birmingham. Experiments were approved by the Oklahoma State University, Human Use Committee, Institutional Review Board.
3
Generating Ccl28-knockdown PAN02 cells
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Mouse pancreatic ductal adenocarcinoma cell line PAN02 was purchased from ATCC and cultured in DMEM with 10% FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific). Ccl28-knockdown (Ccl28KD) PAN02 cells were generated with RNA interference. shRNA target sequences against Ccl28 gene (#1: 5′-GCTGTCATCCTTCATGTTAAA-3′; #2: 5′-CCCGCACAATCGTACTTTGAA-3′) were cloned into pLKO.1-TRC cloning vector [Addgene #10878]. PAN02 cells were transduced with lentiviral vectors encoding a nonsilencing scramble shRNA [shScr] sequence or both shCcl28 sequences. After lentiviral infection, PAN02 cells were selected with 2 μg/ml puromycin for 3 days.
4
Cell Culture of Pancreatic Cancer Lines
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The pancreatic cancer cell lines (Pan02, MIA PaCa-2, BxPC-3, PANC-1, Panc-28, SW1990, Capan-2) were obtained from ATCC (Manassas, VA, USA). CHO and HEK293 were purchased from the National Infrastructure of Cell Line Resource (Shanghai, China). HEK293, Pan02, BxPC-3, PANC-1 and Panc-28 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) with 100 U/mL of penicillin and 100 µg/mL of streptomycin (Gibco, Carlsbad, CA, USA). MIA PaCa-2 was grown in DMEM supplemented with 10% FBS and 2.5% horse serum (Gibco, Carlsbad, CA, USA). SW1990 was maintained in L15 medium supplemented with 10% FBS. Capan-2 was cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) supplemented with 10% FBS. CHO was grown in DMEM-F12 (Gibco, Carlsbad, CA, USA) supplemented with 10% FBS. All cells were maintained at 37 °C in a humidified 5% CO2 atmosphere. Cell lines were identified by short tandem repeat analysis and checked for mycoplasma contamination.
5
Culturing Pancreatic Cancer Cell Lines
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AsPC1 & MIA PaCa2 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), authenticated by STR profiling, and cultured as instructed by the vendor. Pan02 was purchased from ATCC. All culture materials were purchased from Gibco/Life Technology Inc., (Gibco, Grand Island, NY, USA). MMC18 cells, provided by Dr. Gloria Su, were generated from metastatic tumors of mouse pancreatic cancer model [17 (link)], and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS). AsPC1 was cultured in RPMI1640 Medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin and 100 mg/mL streptomycin. Other cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (Gibco, Grand Island, NY, USA), 100 U/mL penicillin and 100 mg/mL streptomycin. The cultures maintained in a humidified incubator at 37 °C and 5% CO2.
6
Cell Line Validation and Characterization
7
Cell Line Validation and Characterization
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8
Generation and Maintenance of Engineered Cell Lines
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The HEK293T- or CHO-overexpressing cell lines were generated by the transduction of 293T or CHO (ATCC) cells with lentivirus from a PD-L1 or 4-1BB full-length sequence encoding vector. The HEK293T-overexpressing cell lines were maintained in DMEM (GIBCO) supplemented with 10% FBS (GIBCO), 50 U/mL penicillin, and 50 mg/mL streptomycin in a humidified atmosphere at 37°C with 5% CO2. The CHO-overexpressing cell lines were maintained in F12K (GIBCO) supplemented with 10% FBS (GIBCO), 50 U/mL penicillin, and 50 mg/mL streptomycin in a humidified atmosphere at 37°C with 5% CO2. NK92MI-Fcγ RIIIA were generated by the transduction of NK92MI cells (Procell) with lentivirus from an FcγRIIIA-GFP-encoding vector and maintained in NK92MI special medium (Procell, CM-0533). MCF7, RKO, NCI-H358, MC38, Pan02, B16F10, and EL-4 were purchased from ATCC and cultured according to manufacturer's guidelines. All cells were cultured for less than 20 passages. Human peripheral blood mononuclear cells (PBMC) were obtained as cryopreserved vials of cells from individual donors from Sailybio Corporation.
9
Cell Lines in Pancreatic Cancer Research
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10
Synthesis and Cytotoxicity Evaluation
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