Phase-contrast and epifluorescence microscopy was performed using Leica DMi8 model, with an HC PL Fluotar 10x/0.30 Dry objective, and images were acquired using Leica LAS X Software, and processed using Fiji Image Analysis Software. Lumencor SpectraX was used as a source of light for excitation, and routed through a Rhodamine excitation filter cube consisting of Excitation 546 nm/ 10 nm (band-pass filter), Dichroic 560 nm (long-pass filter), and Emission 585 nm/ 40 nm (band-pass filter), and acquired using Andor EMCCD iXon Ultra 888 camera.
Hc pl fluotar 10x 0.30 dry objective
Manufactured by Leica
The HC PL Fluotar 10x/0.30 Dry objective is a high-quality optical component designed for use in microscopy. It features a magnification of 10x and a numerical aperture of 0.30. This objective is intended for use in various microscopy applications that require a dry lens configuration.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using hc pl fluotar 10x 0.30 dry objective
1
Fluorescence Microscopy Imaging Protocol
2
Fluorescence Microscopy Imaging Protocol
3
Quantifying Adherent Cells in Seahorse Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the Seahorse experiment the plates were centrifuged (5 minutes at 150 x g) to sediment detached cells and a part of the medium was aspirated from the wells (Table 1). Samples in Seahorse XF Cell Culture Microplates were thereafter scanned in the bright field mode by a monochrome fluorescence CCD camera Leica DFC 350FX mounted on camera port of inverted fully motorized microscope stand Leica DMI 6000. A Leica HC PL FLUOTAR 10x/0, 30 DRY objective was used to acquire tile scans with 4x5 fields with a corresponding pixel size 921x921 nm.
We then used the Fiji software [17] (link) to count cells manually. During cell counting, cells were divided into two groups based on their shape -a round form, with defined and visible edges and flat -not round form, without defined edges or with protrusions.
We then used the Fiji software [17] (link) to count cells manually. During cell counting, cells were divided into two groups based on their shape -a round form, with defined and visible edges and flat -not round form, without defined edges or with protrusions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!