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2 protocols using anti k8.1 sc 65446

1

Inhibition of KSHV Replication

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Doxycycline (Dox), sodium butyrate (NaB), and phosphonoacetic acid (PAA)
were purchased from Sigma. PAA was used at 100 μM to inhibit KSHV
replication. Recombinant human IFNβ was from Peprotech (300–02BC).
The following antibodies were used in our study: anti-FLAG (F1804, Sigma),
anti-tubulin (GTU-88, Sigma), anti-LANA (13–210-100, Advanced
Biotechnologies), anti-ORF45 (sc-53883, Santa Cruz), anti-K8 (sc-57889, Santa
Cruz), anti-K8.1 (sc-65446, Santa Cruz), anti-ORF26 (NBP1–47357, Novus
Biologicals), anti-vIRF3 (NB200–167, Novus Biologicals), anti-CBP
(sc-369, Santa Cruz), anti-IRF3 (sc-33641, Santa Cruz), and anti-pIRF3 (S396)
(4947S, Cell Signaling). Anti-RTA and anti-ORF6 antibodies were generously
provided by Dr. Yoshihiro Izumiya (University of California, Davis) and Dr. Gary
Hayward (Johns Hopkins University), respectively.
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2

Antibody Validation for KSHV Research

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Chemicals were purchased from the following manufacturers: cycloheximide (CHX), doxycycline (Doxy), sodium butyrate (NaB), MG132, and etoposide were obtained from Sigma; A-1210477 was obtained from Cayman Chemical; and AT-101 was procured from Apex Bio. Primary antibodies were purchased from the following manufacturers: anti-MCL-1 (4572), anti-ubiquitin (3933), anti-mTOR (7959), and anti-KSHV LANA (LNA-1; 4013) were purchased from Abcam; anti-FBW7 (A301-720A) was obtained from Bethyl; anti-MCL-1 (sc-12756), anti-GST (sc-138), anti-Lamin A/C (sc-625), anti-ORF45 (sc-53883), and anti-K8.1 (sc-65446) were obtained from Santa Cruz; anti-β-actin (A-5441), anti-FLAG (F1804), and anti-tubulin (GTU-88) were purchased from Sigma; anti-V5 (MA5-15253) was obtained from Invitrogen; anti-Au (901901) was purchased from BioLegend; anti-HA (M180-3) was procured from MBL; anti-Cyclin E (2983) and anti-COXIV (4850) were purchased from cell signaling technology; anti-K2 (13-214-050) was obtained from ABI. Myc antibody was kindly provided by Dr. Jin-Hyun Ahn (Sungkyunkwan University). Anti-RTA and anti-ORF6 antibodies were generously provided by Dr. Yoshihiro Izumiya (University of California, Davis) and Dr. Gary Hayward (Johns Hopkins University), respectively.
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