Ah7614

In total, 40 mM PA solution: PA (Sigma-Aldrich, St. Louis, USA, 0.0614 g) was added to 3 ml NaOH solution (0.1 mol/l), placed in a 75 °C full saponification water bath for 30 min until the PA particles are completely dissolved and the liquid is colorless and transparent. Then the liquid was added to 3 ml BSA (40%, free of fatty acid) solution immediately with sufficient mixing. In total, 100 mM AH7614 solution: AH7614 (TOCRIS, England, 10 mg) was dissolved in 285 μl DMSO. In total, 100 mM GW1100 solution: 5 mg GW1100 (MCE, America, 5 mg) was dissolved in 960 μl DMSO. In total, 10 mM Bay 11-7082 solution: Bay 11-7082 (MCE, America, 5 mg) was dissolved in 2.41 ml DMSO.

The preparation of 40 mM PA solution: PA (Sigma-Aldrich, St. Louis, USA, 0.0614 g) was injected into 3 mL of 0.1 mol/L NaOH solution, and the mixture was placed in a full saponification water bath at 75℃ for half an hour until the PA particles were completely dissolved and the liquid had turned colorless and apparent. Afterward, 3 mL of BSA (40%, free of fatty acid) solution was injected into the liquid followed by thorough mixing. Next, 100 mM AH7614 solution was prepared by dissolving AH7614 (TOCRIS, England, 10 mg) in 285 µL of DMSO. The 100 mM GW1100 solution was prepared by dissolving 5 mg of GW1100 (MCE, America, 5 mg) in 960 µL of DMSO.
Turkish galls (Quercus infectoria Oliv.) is a finished product donated by Professor Han Bo from the School of Pharmacy, Shihezi University. In this study, dry powdered Turkish galls were dissolved in sterile PBS at the required concentration and used for subsequent experiments. The insect galls was identified by Professor Bo Han. Voucher specimens (NO. 20,160,305) were preserved in the School of Pharmacy, Shihezi University.

Differentiated cells were incubated for 1 h at RT with the calcium‐sensitive dye Fluo‐4‐AM (Invitrogen) in Krebs–Ringer bicarbonate buffer (Sigma). Hereafter, the cells were washed twice with buffer, followed by live cell imaging with a confocal laser scanning microscope (LSM 510, Zeiss) and stimulation with TUG‐891 (10 μM), with or without preincubation with the GPR120 antagonist AH7614 for 5 min (100 μM; Tocris) or the Gαq inhibitor YM‐254890 for 30 min (0.1 μM, Alpha Laboratories).